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🆕 Date Issued:
2015
🗄 In DepositOnce:2017-10-25
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H-2-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O-2-tolerant hydrogenase and a P450 monooxygenase

Lonsdale, Thomas H.; Lauterbach, Lars; Malca, Sumire Honda; Nestl, Bettina M.; Hauer, Bernhard; Lenz, Oliver

An in vivo biotransformation system is presented that affords the hydroxylation of n-octane to 1-octanol on the basis of NADH-dependent CYP153A monooxygenase and NAD(+)-reducing hydrogenase heterologously synthesized in a bacterial host. The hydrogenase sustains H-2-driven NADH cofactor regeneration even in the presence of O-2, the co-substrate of monooxygenase.
Published in: Chemical communications, 10.1039/c5cc06078h, Royal Society of Chemistry
Creative Commons Attribution 4.0 (CC BY)Creative Commons Attribution 4.0 (CC BY)
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