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🆕 Date Issued:
1980
🗄 In DepositOnce:2019-01-04
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Isolation of the Phytochrome Chromophore. The Cleavage Reaction with Hydrogen Bromide

Rüdiger, Wolfhart; Brandlmeier, Thomas; Blos, Inge; Gossauer, Albert; Weller, Jens-Peter

The cleavage of the bilin chromophore from C-phycocyanin with hydrogen bromide yields 3E-configurated phycocyanobilin (4) as the major and 3 Z-configurated phycocyanobilin (5) as the minor reaction product. The reaction of synthetic 3E-configurated phytochromobilin (2) with hydrogen bromide and methanol leads only to a methanol adduct at the C-18 side chain (7) whereas the same reaction with the 3Z-configurated phytochromobilin (3) leads to 7 and 2. The bilin chromophore was cleaved also from phytochrome after preparation of phytochromobilin peptides. The detection of 2 and 7 suggested that 3Z-and 3E-configurated phytochromobilin were the primary products of cleavage from phytochrome. A reaction scheme is given which can explain the results of the reaction with hydrogen bromide and methanol.
phytochromebile pigmentsphytochromobilinthioether cleavagec-phycocyanin
Published in: Zeitschrift für Naturforschung C, 10.1515/znc-1980-9-1018, De Gruyter
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