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Main Title: Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
Author(s): Savas, Sumeyra
Ersoy, Aylin
Gulmez, Yakup
Kilic, Selcuk
Levent, Belkis
Altintas, Zeynep
Type: Article
Language Code: en
Abstract: Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL−1. The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection.
Issue Date: 27-Aug-2018
Date Available: 11-Sep-2019
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Subject(s): Salmonella spp.
pathogen detection
antibody biosensor
DNA biosensor
microfluidic-based electrochemical sensor
nanoparticle enhanced bio-detection
infectious diseases
Journal Title: Materials
Publisher: MDPI
Publisher Place: Basel
Volume: 11
Issue: 9
Article Number: 1541
Publisher DOI: 10.3390/ma11091541
EISSN: 1996-1944
Appears in Collections:FG Technische Chemie » Publications

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