Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-8992
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dc.contributor.authorSavas, Sumeyra-
dc.contributor.authorErsoy, Aylin-
dc.contributor.authorGulmez, Yakup-
dc.contributor.authorKilic, Selcuk-
dc.contributor.authorLevent, Belkis-
dc.contributor.authorAltintas, Zeynep-
dc.date.accessioned2019-09-11T15:57:23Z-
dc.date.available2019-09-11T15:57:23Z-
dc.date.issued2018-08-27-
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/10001-
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-8992-
dc.description.abstractBacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL−1. The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection.en
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc540 Chemie und zugeordnete Wissenschaftende
dc.subject.otherSalmonella spp.en
dc.subject.otherpathogen detectionen
dc.subject.otherantibody biosensoren
dc.subject.otherDNA biosensoren
dc.subject.othermicrofluidic-based electrochemical sensoren
dc.subject.othernanoparticle enhanced bio-detectionen
dc.subject.otherinfectious diseasesen
dc.titleNanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonellaen
dc.typeArticleen
dc.date.updated2019-08-01T02:35:54Z-
tub.accessrights.dnbfreeen
tub.publisher.universityorinstitutionTechnische Universität Berlinen
dc.identifier.eissn1996-1944-
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.3390/ma11091541en
dcterms.bibliographicCitation.journaltitleMaterialsen
dcterms.bibliographicCitation.originalpublisherplaceBaselen
dcterms.bibliographicCitation.volume11en
dcterms.bibliographicCitation.originalpublishernameMDPIen
dcterms.bibliographicCitation.issue9en
dcterms.bibliographicCitation.articlenumber1541en
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