Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-9423
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Main Title: Physiological analysis oflactobacillus rhamnosusVTT E‐97800
Subtitle: Adaptive response to osmotic stress induced by trehalose
Author(s): Sunny‐Roberts, Elizabeth Oluwaseun
Knorr, Dietrich
Type: Article
Language Code: en
Abstract: Purpose – This paper aims to describe the physiological analysis of L. rhamnosus VTT E‐97800 and its adaptive response to osmotic stress induced by trehalose. Design/methodology/approach – Cells of L. rhamnosus E800 in the stationary phase of growth were subjected to osmotic stress induced by trehalose treatments. The effects of osmotic stress on the viability of the study strain were determined by conducting flow cytometric analysis with carboxyfluorescein diacetate (cFDA) and propidium iodide (PI) and by observing the corresponding cells growth on MRS agar plates. Osmotic‐induced changes of esterase activity and membrane integrity were monitored. Ability to extrude intracellular accumulated cF (additional vitality marker) was taken into consideration. Findings – The fluorescence‐based approach gave additional insights on osmotic induced changes of cellular events, which could not be explicitly assessed by culture techniques. Trehalose treatments caused a transient membrane permeabilization as revealed by a gradual decrease in esterase activity (a measure of enzyme activity and thus of viability) with increase in trehalose molarity. However, culturability on MRS agar was not significantly affected. Membrane integrity was maintained and there was an improvement in the ability of cells to extrude intracellular accumulated cF. Originality/value – The paper provides a comparative study of the conventional culture techniques and the flow cytometric viability assessment which showed that esterase activity cannot be relied on to ascertain the culturability and viability status of an organism.
URI: https://depositonce.tu-berlin.de/handle/11303/10471
http://dx.doi.org/10.14279/depositonce-9423
Issue Date: 11-Sep-2007
Date Available: 11-Dec-2019
DDC Class: 630 Landwirtschaft und verwandte Bereiche
640 Hauswirtschaft und Familie
660 Chemische Verfahrenstechnik
Subject(s): food preservation
metabolic diseases
micro-organisms
biotechnology
License: http://rightsstatements.org/vocab/InC/1.0/
Journal Title: British Food Journal
Publisher: Emerald
Publisher Place: Bingley 
Volume: 109
Issue: 9
Publisher DOI: 10.1108/00070700710780706
Page Start: 735
Page End: 748
EISSN: 1758-4108
ISSN: 0007-070X
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:FG Lebensmittelbiotechnologie und -prozesstechnik » Publications

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