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dc.contributor.authorPaege, Norman-
dc.contributor.authorWarnecke, Dirk-
dc.contributor.authorZäuner, Simone-
dc.contributor.authorHagen, Silke-
dc.contributor.authorRodrigues, Ana-
dc.contributor.authorBaumann, Birgit-
dc.contributor.authorThiess, Melanie-
dc.contributor.authorJung, Sascha-
dc.contributor.authorMeyer, Vera-
dc.date.accessioned2020-02-13T17:36:57Z-
dc.date.available2020-02-13T17:36:57Z-
dc.date.issued2019-12-11-
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/10778-
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-9673-
dc.description.abstractAFP is an antimicrobial peptide (AMP) produced by the filamentous fungus Aspergillus giganteus and is a very potent inhibitor of fungal growth that does not affect the viability of bacteria, plant, or mammalian cells. It targets chitin synthesis and causes plasma membrane permeabilization in many human- and plant-pathogenic fungi, but its exact mode of action is not known. After adoption of the “damage-response framework of microbial pathogenesis” regarding the analysis of interactions between AMPs and microorganisms, we have recently proposed that the cytotoxic capacity of a given AMP depends not only on the presence/absence of its target(s) in the host and the AMP concentration applied but also on other variables, such as microbial survival strategies. We show here using the examples of three filamentous fungi (Aspergillus niger, Aspergillus fumigatus, and Fusarium graminearum) and two yeasts (Saccharomyces cerevisiae and Pichia pastoris) that the important parameters defining the AFP susceptibilities of these fungi are (i) the presence/absence of glycosylceramides, (ii) the presence/absence of Δ3(E) desaturation of the fatty acid chain therein, and (iii) the (dis)ability of these fungi to respond to AFP inhibitory effects with the fortification of their cell walls via increased chitin and β-(1,3)-glucan synthesis. These observations support the idea of the adoption of the damage-response framework to holistically understand the outcome of AFP inhibitory effects.en
dc.description.sponsorshipTU Berlin, Open-Access-Mittel - 2019en
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherantimicrobial peptideen
dc.subject.otherantifungal protein AFPen
dc.subject.othergamma coreen
dc.subject.otherdamage-response frameworken
dc.subject.othersphingolipiden
dc.subject.otherglycosylceramideen
dc.subject.otherΔ3(E)-desaturaseen
dc.subject.otherfilamentous fungusen
dc.subject.otheryeasten
dc.titleSpecies-Specific Differences in the Susceptibility of Fungi to the Antifungal Protein AFP Depend on C-3 Saturation of Glycosylceramidesen
dc.typeArticleen
tub.accessrights.dnbfreeen
tub.publisher.universityorinstitutionTechnische Universität Berlinen
dc.identifier.eissn2379-5042-
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1128/mSphere.00741-19en
dcterms.bibliographicCitation.journaltitlemSphereen
dcterms.bibliographicCitation.originalpublisherplaceWashington DCen
dcterms.bibliographicCitation.volume4en
dcterms.bibliographicCitation.originalpublishernameAmerican Society for Microbiology (ASM)en
dcterms.bibliographicCitation.issue6en
dcterms.bibliographicCitation.articlenumbere00741-19en
Appears in Collections:FG Angewandte und Molekulare Mikrobiologie » Publications

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