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Main Title: MiR‐375‐mediated suppression of engineered coxsackievirus B3 in pancreatic cells
Author(s): Pryshliak, Markian
Hazini, Ahmet
Knoch, Klaus
Dieringer, Babette
Tolksdorf, Beatrice
Solimena, Michele
Kurreck, Jens
Pinkert, Sandra
Fechner, Henry
Type: Article
Abstract: Coxsackievirus B3 (CVB3) has potential as a new oncolytic agent for the treatment of cancer but can induce severe pancreatitis. Here, we inserted target sequences of the microRNA miR‐375 (miR‐375TS) into the 5′ terminus of the polyprotein encoding sequence or into the 3′UTR of the CVB3 strain rCVB3.1 to prevent viral replication in the pancreas. In pancreatic EndoC‐βH1 cells expressing miR‐375 endogenously, replication of the 5′‐miR‐375TS virus and that of the 3′‐miR‐375TS virus was reduced by 4 × 103‐fold and 3.9 × 104‐fold, respectively, compared to the parental rCVB3.1. In colorectal carcinoma cells, replication and cytotoxicity of both viruses were slightly reduced compared to rCVB3.1, but less pronounced for the 3′‐miR‐375TS virus. Thus, CVB3 with miR‐375TS in the 3′UTR of the viral genome may be suitable to avoid pancreatic toxicity.
Subject(s): cancer therapy
colorectal cancer
coxsackievirus B3
oncolytic virus
Issue Date: 7-Nov-2019
Date Available: 26-Feb-2020
Language Code: en
DDC Class: 570 Biowissenschaften; Biologie
530 Physik
610 Medizin und Gesundheit
Sponsor/Funder: BMBF, 031A331, Verbesserung des CVB3-Maus-Myokarditismodells durch Entwicklung Pankreas-attenuierter Coxsackieviren
TU Berlin, Open-Access-Mittel - 2019
Journal Title: FEBS Letters
Publisher: Wiley
Volume: 594
Issue: 4
Publisher DOI: 10.1002/1873-3468.13647
Page Start: 763
Page End: 775
EISSN: 1873-3468
ISSN: 0014-5793
TU Affiliation(s): Fak. 3 Prozesswissenschaften » Inst. Biotechnologie » FG Angewandte Biochemie
Appears in Collections:Technische Universität Berlin » Publications

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