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Main Title: Single-Use Printed Biosensor for L-Lactate and Its Application in Bioprocess Monitoring
Author(s): Theuer, Lorenz
Randek, Judit
Junne, Stefan
Neubauer, Peter
Mandenius, Carl-Fredrik
Beni, Valerio
Type: Article
Language Code: en
Abstract: There is a profound need in bioprocess manufacturing for low-cost single-use sensors that allow timely monitoring of critical product and production attributes. One such opportunity is screen-printed enzyme-based electrochemical sensors, which have the potential to enable low-cost online and/or off-line monitoring of specific parameters in bioprocesses. In this study, such a single-use electrochemical biosensor for lactate monitoring is designed and evaluated. Several aspects of its fabrication and use are addressed, including enzyme immobilization, stability, shelf-life and reproducibility. Applicability of the biosensor to off-line monitoring of bioprocesses was shown by testing in two common industrial bioprocesses in which lactate is a critical quality attribute (Corynebacterium fermentation and mammalian Chinese hamster ovary (CHO) cell cultivation). The specific response to lactate of the screen-printed biosensor was characterized by amperometric measurements. The usability of the sensor at typical industrial culture conditions was favorably evaluated and benchmarked with commonly used standard methods (HPLC and enzymatic kits). The single-use biosensor allowed fast and accurate detection of lactate in prediluted culture media used in industrial practice. The design and fabrication of the biosensor could most likely be adapted to several other critical bioprocess analytes using other specific enzymes. This makes this single-use screen-printed biosensor concept a potentially interesting and versatile tool for further applications in bioprocess monitoring.
Issue Date: 9-Mar-2020
Date Available: 12-May-2020
DDC Class: 660 Chemische Verfahrenstechnik
Subject(s): lactate biosensor
enzyme electrode
off-line monitoring
at-line measurement
in-line monitoring
Sponsor/Funder: EC/H2020/643056/EU/Rapid Bioprocess Development/Biorapid
Journal Title: Processes
Publisher: MDPI
Publisher Place: Basel
Volume: 8
Issue: 3
Article Number: 321
Publisher DOI: 10.3390/pr8030321
EISSN: 2227-9717
Appears in Collections:FG Bioverfahrenstechnik » Publications

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