Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-10209
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Main Title: Development of a Continuous Pulsed Electric Field (PEF) Vortex-Flow Chamber for Improved Treatment Homogeneity Based on Hydrodynamic Optimization
Author(s): Schottroff, Felix
Knappert, Justus
Eppmann, Pauline
Krottenthaler, Anna
Horneber, Tobias
McHardy, Christopher
Rauh, Cornelia
Jaeger, Henry
Type: Article
Language Code: en
Abstract: Pulsed electric fields (PEF) treatment is an effective process for preservation of liquid products in food and biotechnology at reduced temperatures, by causing electroporation. It may contribute to increase retention of heat-labile constituents with similar or enhanced levels of microbial inactivation, compared to thermal processes. However, especially continuous PEF treatments suffer from inhomogeneous treatment conditions. Typically, electric field intensities are highest at the inner wall of the chamber, where the flow velocity of the treated product is lowest. Therefore, inhomogeneities of the electric field within the treatment chamber and associated inhomogeneous temperature fields emerge. For this reason, a specific treatment chamber was designed to obtain more homogeneous flow properties inside the treatment chamber and to reduce local temperature peaks, therefore increasing treatment homogeneity. This was accomplished by a divided inlet into the chamber, consequently generating a swirling flow (vortex). The influence of inlet angles on treatment homogeneity was studied (final values: radial angle α = 61°; axial angle β = 98°), using computational fluid dynamics (CFD). For the final design, the vorticity, i.e., the intensity of the fluid rotation, was the lowest of the investigated values in the first treatment zone (1002.55 1/s), but could be maintained for the longest distance, therefore providing an increased mixing and most homogeneous treatment conditions. The new design was experimentally compared to a conventional co-linear setup, taking into account inactivation efficacy of Microbacterium lacticum as well as retention of heat-sensitive alkaline phosphatase (ALP). Results showed an increase in M. lacticum inactivation (maximum Δlog of 1.8 at pH 7 and 1.1 at pH 4) by the vortex configuration and more homogeneous treatment conditions, as visible by the simulated temperature fields. Therefore, the new setup can contribute to optimize PEF treatment conditions and to further extend PEF applications to currently challenging products.
URI: https://depositonce.tu-berlin.de/handle/11303/11324
http://dx.doi.org/10.14279/depositonce-10209
Issue Date: 30-Apr-2020
Date Available: 11-Jun-2020
DDC Class: 600 Technik, Technologie
570 Biowissenschaften; Biologie
Subject(s): pulsed electric fields
treatment chamber design
proof of concept
process optimization
numerical simulation
differentiation of thermal and electric field effects
non-thermal inactivation of microorganisms
effects of PEF on alkaline phosphatase
PEF
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Frontiers in Bioengineering and Biotechnology
Publisher: Frontiers Media S.A.
Publisher Place: Lausanne
Volume: 8
Article Number: 340
Publisher DOI: 10.3389/fbioe.2020.00340
EISSN: 2296-4185
Appears in Collections:FG Lebensmittelbiotechnologie und -prozesstechnik » Publications

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