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Main Title: From three‐dimensional morphology to effective diffusivity in filamentous fungal pellets
Author(s): Schmideder, Stefan
Barthel, Lars
Müller, Henri
Meyer, Vera
Briesen, Heiko
Type: Article
Language Code: en
Abstract: Filamentous fungi are exploited as cell factories in biotechnology for the production of proteins, organic acids, and natural products. Hereby, fungal macromorphologies adopted during submerged cultivations in bioreactors strongly impact the productivity. In particular, fungal pellets are known to limit the diffusivity of oxygen, substrates, and products. To investigate the spatial distribution of substances inside fungal pellets, the diffusive mass transport must be locally resolved. In this study, we present a new approach to obtain the effective diffusivity in a fungal pellet based on its three‐dimensional morphology. Freeze‐dried Aspergillus niger pellets were studied by X‐ray microcomputed tomography, and the results were reconstructed to obtain three‐dimensional images. After processing these images, representative cubes of the pellets were subjected to diffusion computations. The effective diffusion factor and the tortuosity of each cube were calculated using the software GeoDict. Afterwards, the effective diffusion factor was correlated with the amount of hyphal material inside the cubes (hyphal fraction). The obtained correlation between the effective diffusion factor and hyphal fraction shows a large deviation from the correlations reported in the literature so far, giving new and more accurate insights. This knowledge can be used for morphological optimization of filamentous pellets to increase the yield of biotechnological processes.
Issue Date: 8-Oct-2019
Date Available: 30-Oct-2020
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): Aspergillus niger
effective diffusion
filamentous fungal pellets
X‐ray microcomputed tomography
Sponsor/Funder: DFG, 198187031, Mikro-Computertomograph mit integrierter Materialprüfmaschine und Kühleinheit
DFG, 315384307, Verallgemeinerte morphologische Modellierung aggregierender, filamentöser Mikroorganismen
DFG, 315305620, Untersuchung des Einflusses von Scherkräften auf das morphogenetische Gennetzwerk, die Zellintegrität, mikroskopische und makroskopische Morphologie von Aspergillus niger sowie Bildungsraten intra- und extrazellulärer Produkte
Journal Title: Biotechnology and Bioengineering
Publisher: Wiley
Publisher Place: New York, NY
Volume: 116
Issue: 12
Publisher DOI: 10.1002/bit.27166
Page Start: 3360
Page End: 3371
EISSN: 1097-0290
ISSN: 0006-3592
Appears in Collections:FG Angewandte und Molekulare Mikrobiologie » Publications

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