Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-10762
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Main Title: Spectral Unmixing‐Based Reaction Monitoring of Transformations between Nucleosides and Nucleobases†
Author(s): Kaspar, Felix
Giessmann, Robert T.
Westarp, Sarah
Hellendahl, Katja F.
Krausch, Niels
Thiele, Isabel
Walczak, Miriam C.
Neubauer, Peter
Wagner, Anke
Type: Article
Language Code: en
Abstract: The increased interest in (enzymatic) transformations between nucleosides and nucleobases has demanded the development of efficient analytical tools. In this report, we present an update and extension of our recently described method for monitoring these reactions by spectral unmixing. The presented method uses differences in the UV absorption spectra of nucleosides and nucleobases after alkaline quenching to derive their ratio based on spectral shape by fitting normalized reference spectra. It is applicable to a broad compound spectrum comprising more than 35 examples, offers HPLC‐like accuracy, ease of handling and significant reductions in both cost and data acquisition time compared to other methods. This contribution details the principle of monitoring reactions by spectral unmixing, gives recommendations regarding solutions to common problems and applications that necessitate special sample treatment. We provide software, workflows and reference spectra that facilitate the straightforward and versatile application of the method.
URI: https://depositonce.tu-berlin.de/handle/11303/11872
http://dx.doi.org/10.14279/depositonce-10762
Issue Date: 18-Jun-2020
Date Available: 9-Nov-2020
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Subject(s): nucleobases
nucleoside phosphorylase
nucleosides
spectral unmixing
UV/Vis spectroscopy
Sponsor/Funder: DFG, 390540038, EXC 2008: Unifying Systems in Catalysis "UniSysCat"
TU Berlin, Open-Access-Mittel – 2020
DFG, 392246628, Chemo-enzymatische Synthese von Selen-modifizierten Nukleosiden, Nukleotiden und Oligonukleotiden
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: ChemBioChem
Publisher: Wiley
Publisher Place: New York, NY
Volume: 21
Issue: 18
Publisher DOI: 10.1002/cbic.202000204
Page Start: 2604
Page End: 2610
EISSN: 1439-7633
ISSN: 1439-4227
Appears in Collections:FG Bioverfahrenstechnik » Publications

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