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Main Title: Fabrication, in vitro and in vivo studies of bilayer composite membrane for periodontal guided tissue regeneration
Author(s): Zahid, Saba
Khan, Abdul Samad
Chaudhry, Aqif Anwar
Ghafoor, Sarah
Ain, Qurat Ul
Raza, Ahtasham
Rahim, Muhammad Imran
Görke, Oliver
Rehman, Ihtesham Ur
Tufail, Asma
Type: Article
Abstract: Development of a guided occlusive biodegradable membrane with controlled morphology in order to restrict the ingrowth of epithelial cells is still a challenge in dental tissue engineering. A bilayer membrane with a non-porous upper layer (polyurethane) and porous lower layer (polycaprolactone and bioactive glass composite) with thermoelastic properties to sustain surgery treatment was developed by lyophilization. Morphology, porosity, and layers attachment were controlled by using the multi-solvent system. In vitro and in vivo biocompatibility, cell attachment, and cell proliferation were analyzed by immunohistochemistry and histology. The cell proliferation rate and cell attachment results showed good biocompatibility of both surfaces, though cell metabolic activity was better on the polycaprolactone-bioactive glass surface. Furthermore, the cells were viable, adhered, and proliferated well on the lower porous bioactive surface, while non-porous polyurethane surface demonstrated low cell attachment, which was deliberately designed and a pre-requisite for guided tissue regeneration/guided bone regeneration membranes. In addition, in vivo studies performed in a rat model for six weeks revealed good compatibility of membranes. Histological analysis (staining with hematoxylin and eosin) indicated no signs of inflammation or accumulation of host immune cells. These results suggested that the fabricated biocompatible bilayer membrane has the potential for use in periodontal tissue regeneration.
Subject(s): guided tissue regeneration
bioactive glass
bilayer membrane
in vitro cytotoxicity
animal testing
Issue Date: 3-Dec-2018
Date Available: 12-Nov-2020
Language Code: en
DDC Class: 610 Medizin und Gesundheit
Journal Title: Journal of Biomaterials Applications
Publisher: SAGE
Volume: 33
Issue: 7
Publisher DOI: 10.1177/0885328218814986
Page Start: 967
Page End: 978
EISSN: 1530-8022
ISSN: 0885-3282
Notes: This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
TU Affiliation(s): Fak. 3 Prozesswissenschaften » Inst. Werkstoffwissenschaften und -technologien » FG Keramische Werkstoffe
Appears in Collections:Technische Universität Berlin » Publications

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