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Main Title: Epigenetic variability confounds transcriptome but not proteome profiling for coexpression-based gene function prediction
Author(s): Grabowski, Piotr
Kustatscher, Georg
Rappsilber, Juri
Type: Article
Is Part Of: 10.14279/depositonce-9070
Language Code: en
Abstract: Genes are often coexpressed with their genomic neighbors, even if these are functionally unrelated. For small expression changes driven by genetic variation within the same cell type, non-functional mRNA coexpression is not propagated to the protein level. However, it is unclear if protein levels are also buffered against any non-functional mRNA coexpression accompanying large, regulated changes in the gene expression program, such as those occurring during cell differentiation. Here, we address this question by analyzing mRNA and protein expression changes for housekeeping genes across 20 mouse tissues. We find that a large proportion of mRNA coexpression is indeed non-functional and does not lead to coexpressed proteins. Chromosomal proximity of genes explains a proportion of this nonfunctional mRNA coexpression. However, the main driver of non-functional mRNA coexpression across mouse tissues is epigenetic similarity. Both factors together provide an explanation for why monitoring protein coexpression outperforms mRNA coexpression data in gene function prediction. Furthermore, this suggests that housekeeping genes translocating during evolution within genomic subcompartments might maintain their broad expression pattern.
Issue Date: 24-Jul-2018
Date Available: 18-Nov-2020
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): systems biology
genome organisation
gene function prediction
gene expression variability
computational Biology
chromatin function
gene expression
Journal Title: Molecular and Cellular Proteomics
Publisher: American Society for Biochemistry and Molecular Biology
Publisher Place: Bethesda, Md.
Volume: 17
Issue: 11
Publisher DOI: 10.1074/mcp.RA118.000935
Page Start: 2082
Page End: 2090
EISSN: 1535-9484
ISSN: 1535-9476
Appears in Collections:FG Bioanalytik » Publications

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