Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-10904
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Main Title: Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
Author(s): Reddy, Hemanth K.N.
Yoon, Chun Hong
Aquila, Andrew
Awel, Salah
Ayyer, Kartik
Barty, Anton
Berntsen, Peter
Bielecki, Johan
Bobkov, Sergey
Bucher, Maximilian
Carini, Gabriella A.
Carron, Sebastian
Chapman, Henry
Daurer, Benedikt
DeMirci, Hasan
Ekeberg, Tomas
Fromme, Petra
Hajdu, Janos
Hanke, Max Felix
Hart, Philip
Hogue, Brenda G.
Hosseinizadeh, Ahmad
Kim, Yoonhee
Kirian, Richard A.
Kurta, Ruslan P.
Larsson, Daniel S.D.
Duane Loh, N.
Maia, Filipe R.N.C.
Mancuso, Adrian P.
Mühlig, Kerstin
Munke, Anna
Nam, Daewoong
Nettelblad, Carl
Ourmazd, Abbas
Rose, Max
Schwander, Peter
Seibert, Marvin
Sellberg, Jonas A.
Song, Changyong
Spence, John C.H.
Svenda, Martin
Van der Schot, Gijs
Vartanyants, Ivan A.
Williams, Garth J.
Xavier, P. Lourdu
Type: Article
Language Code: en
Abstract: Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65–70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
URI: https://depositonce.tu-berlin.de/handle/11303/12024
http://dx.doi.org/10.14279/depositonce-10904
Issue Date: 27-Jun-2017
Date Available: 19-Nov-2020
DDC Class: 530 Physik
Subject(s): X-ray Free Electron Lasers
XFELs
Single Particle Imaging Initiative
SPI
molecule
X-ray
Atomic Molecular Optics
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Scientific Data
Publisher: Nature Publishing Group
Publisher Place: London
Volume: 4
Article Number: 170079
Publisher DOI: 10.1038/sdata.2017.79
EISSN: 2052-4463
Appears in Collections:Inst. Optik und Atomare Physik » Publications

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