Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-11012
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dc.contributor.authorBartels, Moritz-
dc.contributor.authorGutschmann, Björn-
dc.contributor.authorWidmer, Torsten-
dc.contributor.authorGrimm, Thomas-
dc.contributor.authorNeubauer, Peter-
dc.contributor.authorRiedel, Sebastian L.-
dc.date.accessioned2020-12-08T12:03:09Z-
dc.date.available2020-12-08T12:03:09Z-
dc.date.issued2020-08-07-
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/12138-
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-11012-
dc.description.abstractBiodegradable and biocompatible polyhydroxyalkanoates (PHAs) are promising alternatives to conventional plastics. Based on the chain length of their monomers they are classified as short chain length (scl-) or medium chain length (mcl-) PHA polymers. The type of monomers, the composition and the molecular weight (MW) define the polymer properties. To accelerate the use of PHA as a bulk material, the downstream associated costs need to be minimized. This study focuses on the evaluation of non-halogenated solvents, especially acetone as a scl-PHA non-solvent, for the recovery of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) – P(HB-co-HHx) – with an mcl-HHx content >15 mol% and a MW average (Mw) < 2 × 105 Da. Solvents and precipitants were chosen regarding zeotrope formation, boiling point differences, and toxicity. Non-halogenated solvent-precipitant pairs were evaluated regarding the MW characteristics (MWCs) of the extracted polymer. Acetone and 2-propanol as a low toxic and zeotropic solvent-precipitant pair was evaluated at different extraction temperatures and multiple extraction times. The extraction process was further evaluated by using impure acetone for the extraction and implementing a multi-stage extraction process. Additionally, P(HB-co-HHx) extracted with three different solvents was characterized by 1H and 13C-APT NMR. The screening of precipitants resulted in a negative influence on the MWCs by ethanol precipitation for extractions with acetone and ethyl acetate, respectively. It was observed, that extractions with acetone at 70°C extracted a higher fraction of PHA from the cells compared to extractions at RT, but the Mw was decreased by 9% in average. Acetone with a 2-propanol fraction of up to 30% was still able to extract the polymer 95% as efficient as pure acetone. Additionally, when acetone and ethyl acetate were used in a multi-stage extraction process, a two-stage process was sufficient to extract 98–99% of the polymer from the cells. 1H and 13C-APT NMR analysis confirmed the monomer fraction and structure of the extracted polymers and revealed a random copolymer structure. The presented strategy can be further developed to an ecological and economically feasible PHA downstream process and thus contributes to the commercialization of low-cost PHAs.en
dc.description.sponsorshipTU Berlin, Open-Access-Mittel – 2020en
dc.description.sponsorshipBMBF, 031B0001C, Bioökonomie International 2014: PHABIO APP - Polyhydroxyalkanoate Biopolymere aus tierischen Abfallfetten für die Produktion von wertschöpfendem, biobasierten und abbauendem Plastik; Teilprojekt Cen
dc.description.sponsorshipBMBF, 031B0001B, Bioökonomie International 2014: PHABIO APP - Polyhydroxyalkanoate Biopolymere aus tierischen Abfallfetten für die Produktion von wertschöpfendem, biobasierten und abbauendem Plastik; Teilprojekt Ben
dc.description.sponsorshipBMBF, 031B0798C, Bioökonomie International 2017: PHABio-up - Polyhydroxyalkanoate Biopolymere aus tierischen Abfallfetten: Scale-up zur Realisierung einer industriellen Produktion - Teilvorhaben: Feedstock und Produktextraktionen
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherpolyhydroxyalkanoateen
dc.subject.otherrecoveryen
dc.subject.othernon-halogenated solventsen
dc.subject.othermulti-stage extractionen
dc.subject.othermolecular weighten
dc.subject.othermedium chain length PHAen
dc.subject.otherP(HB-co-HHx)en
dc.subject.otherRalstonia eutrophaen
dc.titleRecovery of the PHA Copolymer P(HB-co-HHx) With Non-halogenated Solvents: Influences on Molecular Weight and HHx-Contenten
dc.typeArticleen
dc.date.updated2020-08-07T15:20:31Z-
tub.accessrights.dnbfreeen
tub.publisher.universityorinstitutionTechnische Universität Berlinen
dc.identifier.eissn2296-4185-
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.3389/fbioe.2020.00944en
dcterms.bibliographicCitation.journaltitleFrontiers in Bioengineering and Biotechnologyen
dcterms.bibliographicCitation.originalpublisherplaceLausanneen
dcterms.bibliographicCitation.volume8en
dcterms.bibliographicCitation.originalpublishernameFrontiersen
dcterms.bibliographicCitation.articlenumber944en
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