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Main Title: Enzymatic Modification of Granular Potato Starch Using Isoamylase—Investigation of Morphological, Physicochemical, Molecular, and Techno‐Functional Properties
Author(s): Ulbrich, Marco
Asiri, Saeed A.
Bussert, Robert
Flöter, Eckhard
Type: Article
Abstract: Granular potato starch is modified using the debranching enzyme isoamylase. The modification is performed in aqueous suspension (40% w/w) at 35 °C by grading the volume (100, 250, and 400 µL/50 g of starch, 200 U · mL−1) of enzyme solution added. The starch products obtained are comprehensively investigated in terms of morphological (scanning electron microscopy), structural (X‐ray diffraction), thermal (differential scanning calorimetry), techno‐functional (solubility, hot paste viscosity, gel strength), and molecular properties (size exclusion chromatography‐multi angle laser light scattering‐differential refractive index detection). The granular integrity is basically preserved after modification and a molecular degradation predominantly of the amylopectin by debranching is proved. However, a slight reduction of the weight average molar mass of the amylose fraction is found too. In addition, the intended partial molecular degradation of the starch polysaccharides, the effect of the preparation procedure including washing with ethanol and grinding impacts several starch characteristics conspicuously.
Subject(s): hot paste viscosity
modified granular starches
potato starches
Issue Date: 27-Sep-2020
Date Available: 16-Dec-2020
Language Code: en
DDC Class: 660 Chemische Verfahrenstechnik
Sponsor/Funder: TU Berlin, Open-Access-Mittel – 2020
Journal Title: Starch ‐ Stärke
Publisher: Wiley
Article Number: 2000080
Publisher DOI: 10.1002/star.202000080
EISSN: 1521-379X
ISSN: 0038-9056
TU Affiliation(s): Fak. 3 Prozesswissenschaften » Inst. Lebensmitteltechnologie und Lebensmittelchemie » FG Lebensmittelverfahrenstechnik
Appears in Collections:Technische Universität Berlin » Publications

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