Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-11102
For citation please use:
Main Title: Episodic Decrease in Temperature Increases mcy Gene Transcription and Cellular Microcystin in Continuous Cultures of Microcystis aeruginosa PCC 7806
Author(s): Martin, Robbie M.
Moniruzzaman, Mohammad
Stark, Gwendolyn F.
Gann, Eric R.
Derminio, Dominique S.
Wei, Bofan
Hellweger, Ferdi L.
Pinto, Ameet
Boyer, Gregory L.
Wilhelm, Steven W.
Type: Article
Language Code: en
Abstract: Microcystins produced during harmful cyanobacterial blooms are a public health concern. Although patterns are emerging, the environmental cues that stimulate production of microcystin remain confusing, hindering our ability to predict fluctuations in bloom toxicity. In earlier work, growth at cool temperatures relative to optimum (18°C vs. 26°C) was confirmed to increase microcystin quota in batch cultures of Microcystis aeruginosa NIES-843. Here, we tested this response in M. aeruginosa PCC 7806 using continuous cultures to examine temporal dynamics and using RNA-sequencing to investigate the physiological nature of the response. A temperature reduction from 26 to 19°C increased microcystin quota ∼2-fold, from an average of ∼464 ag μm–3 cell volume to ∼891 ag μm–3 over a 7–9 d period. Reverting the temperature to 26°C returned the cellular microcystin quota to ∼489 ag μm–3. Long periods (31–42 d) at 19°C did not increase or decrease microcystin quota beyond that observed at 7–9 d. Nitrogen concentration had little effect on the overall response. RNA sequencing indicated that the decrease in temperature to 19°C induced a classic cold-stress response in M. aeruginosa PCC 7806, but this operated on a different timescale than the increased microcystin production. Microcystin quota showed a strong 48- to 72-h time-lag correlation to mcy gene expression, but no correlation to concurrent mcy expression. This work confirms an effect of temperature on microcystin quota and extends our understanding of the physiological nature of the response.
URI: https://depositonce.tu-berlin.de/handle/11303/12227
http://dx.doi.org/10.14279/depositonce-11102
Issue Date: 3-Dec-2020
Date Available: 17-Dec-2020
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): microcystin
temperature
cyanotoxins
cyanobacteria
episodic events
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Frontiers in Microbiology
Publisher: Frontiers
Publisher Place: Lausanne
Volume: 11
Article Number: 601864
Publisher DOI: 10.3389/fmicb.2020.601864
EISSN: 1664-302X
Appears in Collections:FG Wasserreinhaltung » Publications

Files in This Item:
fmicb-11-601864.pdf
Format: Adobe PDF | Size: 1.52 MB
DownloadShow Preview
Thumbnail
Data_Sheet_1.pdf
Format: Adobe PDF | Size: 653.62 kB
DownloadShow Preview
Thumbnail
fmicb-11-601864-g001.tif
Format: TIFF | Size: 709.82 kB
Download
Thumbnail
fmicb-11-601864-g002.tif
Format: TIFF | Size: 2.15 MB
Download
Thumbnail
fmicb-11-601864-g003.tif
Format: TIFF | Size: 810.88 kB
Download
Thumbnail
fmicb-11-601864-g004.tif
Format: TIFF | Size: 713.86 kB
Download
Thumbnail
fmicb-11-601864-g005.tif
Format: TIFF | Size: 1.77 MB
Download
Thumbnail
Table_1.XLSX
Format: Microsoft Excel XML | Size: 11.26 kB
Download
Table_2.XLSX
Format: Microsoft Excel XML | Size: 106.89 kB
Download
Table_3.XLSX
Format: Microsoft Excel XML | Size: 39.67 kB
Download
Table_4.XLSX
Format: Microsoft Excel XML | Size: 40.89 kB
Download
Table_5.XLSX
Format: Microsoft Excel XML | Size: 47.85 kB
Download

Item Export Bar

This item is licensed under a Creative Commons License Creative Commons