Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-11205
For citation please use:
Main Title: Dual Element (C/Cl) Isotope Analysis Indicates Distinct Mechanisms of Reductive Dehalogenation of Chlorinated Ethenes and Dichloroethane in Dehalococcoides mccartyi Strain BTF08 With Defined Reductive Dehalogenase Inventories
Author(s): Franke, Steffi
Seidel, Katja
Adrian, Lorenz
Nijenhuis, Ivonne
Type: Article
Language Code: en
Abstract: Dehalococcoides mccartyi strain BTF08 has the unique property to couple complete dechlorination of tetrachloroethene and 1,2-dichloroethane to ethene with growth by using the halogenated compounds as terminal electron acceptor. The genome of strain BTF08 encodes 20 genes for reductive dehalogenase homologous proteins (RdhA) including those described for dehalogenation of tetrachloroethene (PceA, PteA), trichloroethene (TceA) and vinyl chloride (VcrA). Thus far it is unknown under which conditions the different RdhAs are expressed, what their substrate specificity is and if different reaction mechanisms are employed. Here we found by proteomic analysis from differentially activated batches that PteA and VcrA were expressed during dechlorination of tetrachloroethene to ethene, while TceA was expressed during 1,2-dichloroethane dehalogenation. Carbon and chlorine compound-specific stable isotope analysis suggested distinct reaction mechanisms for the dechlorination of (i) cis-dichloroethene and vinyl chloride versus (ii) tetrachloroethene. This differentiation was observed independent of the expressed RdhA proteins. Differently, two stable isotope fractionation patterns were observed for 1,2-dichloroethane transformation, for cells with distinct RdhA inventories. Conclusively, we could link specific RdhA expression with functions and provide an insight into the apparently substrate-specific reaction mechanisms in the pathway of reductive dehalogenation in D. mccartyi strain BTF08. Data are available via ProteomeXchange with identifiers PXD018558 and PXD018595.
URI: https://depositonce.tu-berlin.de/handle/11303/12365
http://dx.doi.org/10.14279/depositonce-11205
Issue Date: 17-Jul-2020
Date Available: 7-Jan-2021
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): compound-specific stable isotope analysis
nLC-MS/MS
Dehalococcoides mccartyi strain BTF08
reductive dehalogenation
2-dichloroethane
tetrachloroethene
cis-dichloroethene
vinyl chloride
Sponsor/Funder: DFG, 171475307, FOR 1530: Anaerobic Biological Dehalogenation: Organisms, Biochemistry, and (Eco-)physiology
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Frontiers in Microbiology
Publisher: Frontiers
Publisher Place: Lausanne
Volume: 11
Article Number: 1507
Publisher DOI: 10.3389/fmicb.2020.01507
EISSN: 1664-302X
Appears in Collections:FG Geobiotechnologie » Publications

Files in This Item:
fmicb-11-01507.pdf
Format: Adobe PDF | Size: 856.47 kB
DownloadShow Preview
Thumbnail
Data_Sheet_1.PDF
Format: Adobe PDF | Size: 406.61 kB
DownloadShow Preview
Thumbnail
fmicb-11-01507-g001.tif
Format: TIFF | Size: 1.74 MB
Download
Thumbnail
fmicb-11-01507-g002.tif
Format: TIFF | Size: 2.19 MB
Download
Thumbnail
fmicb-11-01507-t001.jpg
Format: JPEG | Size: 206.5 kB
Download
Thumbnail

Item Export Bar

This item is licensed under a Creative Commons License Creative Commons