Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-11236
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Main Title: Interaction of Proteins with a Planar Poly(acrylic acid) Brush: Analysis by Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)
Author(s): Walkowiak, Jacek
Gradzielski, Michael
Zauscher, Stefan
Ballauff, Matthias
Type: Article
Language Code: en
Abstract: We describe the preparation of a poly(acrylic acid) (PAA) brush, polymerized by atom transfer radical polymerization (ATRP) of tert-butyl acrylate (tBA) and subsequent acid hydrolysis, on the flat gold surfaces of quartz-crystal microbalance (QCM) crystals. The PAA brushes were characterized by Fourier transform infrared (FT-IR) spectroscopy, ellipsometry and water contact angle analysis. The interaction of the PAA brushes with human serum albumin (HSA) was studied for a range of ionic strengths and pH conditions by quartz-crystal microbalance with dissipation monitoring (QCM-D). The quantitative analysis showed a strong adsorption of protein molecules onto the PAA brush. By increasing the ionic strength, we were able to release a fraction of the initially bound HSA molecules. This finding highlights the importance of counterions in the polyelectrolyte-mediated protein adsorption/desorption. A comparison with recent calorimetric studies related to the binding of HSA to polyelectrolytes allowed us to fully analyze the QCM data based on the results of the thermodynamic analysis of the binding process.
URI: https://depositonce.tu-berlin.de/handle/11303/12395
http://dx.doi.org/10.14279/depositonce-11236
Issue Date: 30-Dec-2020
Date Available: 11-Jan-2021
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Subject(s): polyelectrolyte brush
protein
ATRP
QCM-D
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Polymers
Publisher: MDPI
Publisher Place: Basel
Volume: 13
Issue: 1
Article Number: 122
Publisher DOI: 10.3390/polym13010122
EISSN: 2073-4360
Appears in Collections:FG Physikalische Chemie / Molekulare Materialwissenschaften » Publications

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