Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-11293
For citation please use:
Main Title: A silver bullet in a golden age of functional genomics: the impact of Agrobacterium-mediated transformation of fungi
Author(s): Idnurm, Alexander
Bailey, Andy M.
Cairns, Timothy C.
Elliott, Candace E.
Foster, Gary D.
Ianiri, Giuseppe
Jeon, Junhyun
Type: Article
Language Code: en
Abstract: The implementation of Agrobacterium tumefaciens as a transformation tool revolutionized approaches to discover and understand gene functions in a large number of fungal species. A. tumefaciens mediated transformation (AtMT) is one of the most transformative technologies for research on fungi developed in the last 20 years, a development arguably only surpassed by the impact of genomics. AtMT has been widely applied in forward genetics, whereby generation of strain libraries using random T-DNA insertional mutagenesis, combined with phenotypic screening, has enabled the genetic basis of many processes to be elucidated. Alternatively, AtMT has been fundamental for reverse genetics, where mutant isolates are generated with targeted gene deletions or disruptions, enabling gene functional roles to be determined. When combined with concomitant advances in genomics, both forward and reverse approaches using AtMT have enabled complex fungal phenotypes to be dissected at the molecular and genetic level. Additionally, in several cases AtMT has paved the way for the development of new species to act as models for specific areas of fungal biology, particularly in plant pathogenic ascomycetes and in a number of basidiomycete species. Despite its impact, the implementation of AtMT has been uneven in the fungi. This review provides insight into the dynamics of expansion of new research tools into a large research community and across multiple organisms. As such, AtMT in the fungi, beyond the demonstrated and continuing power for gene discovery and as a facile transformation tool, provides a model to understand how other technologies that are just being pioneered, e.g. CRISPR/Cas, may play roles in fungi and other eukaryotic species.
URI: https://depositonce.tu-berlin.de/handle/11303/12451
http://dx.doi.org/10.14279/depositonce-11293
Issue Date: 26-Sep-2017
Date Available: 18-Jan-2021
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): functional genomics
mycota
pathogenicity genes
Rhizobium radiobacter
transfer DNA
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Fungal Biology and Biotechnology
Publisher: BioMed Central
Publisher Place: London
Volume: 4
Article Number: 6
Publisher DOI: 10.1186/s40694-017-0035-0
EISSN: 2054-3085
Appears in Collections:FG Angewandte und Molekulare Mikrobiologie » Publications

Files in This Item:
Idnurm_etal_Silver_2017.pdf
Format: Adobe PDF | Size: 1.75 MB
DownloadShow Preview
Thumbnail

Item Export Bar

This item is licensed under a Creative Commons License Creative Commons