Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-11572
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Main Title: Antiinflammatory Medicinal Plants from the Ugandan Greater Mpigi Region Act as Potent Inhibitors in the COX-2/PGH2 Pathway
Author(s): Schultz, Fabien
Osuji, Ogechi Favour
Wack, Barbara
Anywar, Godwin
Garbe, Leif-Alexander
Type: Article
Language Code: en
Abstract: Our study investigates 16 medicinal plants via assessment of inhibition of proinflammatory enzymes such as cyclooxygenases (COX). The plants are used by traditional healers in the Greater Mpigi region in Uganda to treat inflammation and related disorders. We present results of diverse in vitro experiments performed with 76 different plant extracts, namely, (1) selective COX-2 and COX-1 inhibitor screening; (2) 15-LOX inhibition screening; (3) antibacterial resazurin assay against multidrug-resistant Staphylococcus aureus, Listeria innocua, Listeria monocytogenes, and Escherichia coli K12; (4) DPPH assay for antioxidant activity; and (5) determination of the total phenolic content (TPC). Results showed a high correlation between traditional use and pharmacological activity, e.g., extracts of 15 out of the 16 plant species displayed significant selective COX-2 inhibition activity in the PGH2 pathway. The most active COX-2 inhibitors (IC50 < 20 µg/mL) were nine extracts from Leucas calostachys, Solanum aculeastrum, Sesamum calycinum subsp. angustifolium, Plectranthus hadiensis, Morella kandtiana, Zanthoxylum chalybeum, and Warburgia ugandensis. There was no counteractivity between COX-2 and 15-LOX inhibition in these nine extracts. The ethyl acetate extract of Leucas calostachys showed the lowest IC50 value with 0.66 µg/mL (COX-2), as well as the most promising selectivity ratio with 0.1 (COX-2/COX-1). The TPCs and the EC50 values for DPPH radical scavenging activity showed no correlation with COX-2 inhibitory activity. This led to the assumption that the mechanisms of action are most likely not based on scavenging of reactive oxygen species and antioxidant activities. The diethyl ether extract of Harungana madagascariensis stem bark displayed the highest growth inhibition activity against S. aureus (MIC value: 13 µg/mL), L. innocua (MIC value: 40 µg/mL), and L. monocytogenes (MIC value: 150 µg/mL). This study provides further evidence for the therapeutic use of the previously identified plants used medicinally in the Greater Mpigi region.
URI: https://depositonce.tu-berlin.de/handle/11303/12772
http://dx.doi.org/10.14279/depositonce-11572
Issue Date: 12-Feb-2021
Date Available: 10-Mar-2021
DDC Class: 610 Medizin und Gesundheit
Subject(s): inflammation
antibiotics
ethnopharmacology
traditional medicine
pain
fever
cyclooxygenase
lipoxygenase
Albizia coriaria
Cassine buchananii
Combretum molle
Erythrina abyssinica
Ficus saussureana
Harungana madagascariensis
Leucas calostachys
Microgramma lycopodioides
Morella kandtiana
Plectranthus hadiensis
Securidaca longipedunculata
Sesamum calycinum subsp. angustifolium
Solanum aculeastrum
Toddalia asiatica
Warburgia ugandensis
Zanthoxylum chalybeum
Sponsor/Funder: BMBF, 13FH066PX5, FHprofUnt 2015: Modifikation von Proteinen bei der technischen Prozessierung von Melasse und Vinasse: Einfluss auf die Produktion von Bioethanol und Biogas
DFG, 414051096, Open Access Publizieren 2020 - 2021 / Hochschule Neubrandenburg
BMBF, 13FH026IX5, IngenieurNachwuchs 2015: Schutz der Gesundheit durch Einsatz biologischer Fungizide in der Landwirtschaft - Anwendung von Trihydroxy-octadecensäuren (TriOH) als natürliche Pflanzenschutzmittel zur Sicherung einer gesunden Ernährung (OxiLiFungi)
License: https://creativecommons.org/licenses/by/4.0/
Journal Title: Plants
Publisher: MDPI
Publisher Place: Basel
Volume: 10
Issue: 2
Article Number: 351
Publisher DOI: 10.3390/plants10020351
EISSN: 2223-7747
Appears in Collections:FG Bioanalytik » Publications

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