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Main Title: Electrochemical Immunomagnetic Ochratoxin A Sensing: Steps Forward in the Application of 3,3’,5,5’‐Tetramethylbenzidine in Amperometric Assays
Author(s): Höfs, Soraya
Hülagü, Deniz
Bennet, Francesca
Carl, Peter
Flemig, Sabine
Schmid, Thomas
Schenk, Jörg A.
Hodoroaba, Vasile‐Dan
Schneider, Rudolf J.
Type: Article
Abstract: Electrochemical methods offer great promise in meeting the demand for user‐friendly on‐site devices for monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme‐linked immunosorbent assays, with photometric detection of 3,3’,5,5’‐tetramethylbenzidine (TMB), form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphone‐based, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis for sensing of further analytes.
Subject(s): amperometry
cyclic voltammetry
screen-printed electrodes
Issue Date: 12-Jul-2021
Date Available: 17-Sep-2021
Language Code: en
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Journal Title: ChemElectroChem
Publisher: Wiley
Volume: 8
Issue: 13
Publisher DOI: 10.1002/celc.202100446
Page Start: 2597
Page End: 2606
EISSN: 2196-0216
TU Affiliation(s): Fak. 3 Prozesswissenschaften » Inst. Werkstoffwissenschaften und -technologien » FG Keramische Werkstoffe
Appears in Collections:Technische Universität Berlin » Publications

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