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Main Title: Automatic 3D-graph construction of nerve cells from confocal microscope scans
Author(s): Obermayer, Klaus
Scholz, Michael
Dima, Anca
Type: Preprint
Language: English
Language Code: en
Abstract: We present a method of automatic graph construction for the description of the geometric structure of nerve cells from 3-D confocal scans. The method consists of tracing the branch center points, in the branch axial direction using as hints the location of difficult regions inside the neuronal branches. The axis were obtained in previous work by computing pairwise vector products of intersecting gradients associated with across-scales validated boundary edge points of the neuronal branches. The axis anchor points are the branch center points, which are estimated as the “center of mass” of all intersecting gradient end points. The difficult regions are the axis anchor points having a high directional variance of vector products contributing to the associated axis. The presented algorithm, which uses all the information obtained from preprocessing, is robust to variable contrast; has little sensitivity to boundary irregularities; is adaptive to variability of branch geometry; and produces a sparse, topology preserving graph of the neuron under investigation. A subsequent surface reconstruction based on this graph (Schmitt et al., 2001) accompanied by the labeling of the graph with geometric measurements would be feasible.
URI: urn:nbn:de:kobv:83-opus4-10060
Issue Date: 2003
Date Available: 15-Sep-2005
DDC Class: 004 Datenverarbeitung; Informatik
Subject(s): Automatischer Diagrammaufbau
Confocale Mikroskopie
Neuronale Daten
Automatic graph construction
Confocal microscopy
Multiscale image decomposition
Neuronal data
Journal Title: Journal of Electronic Imaging
Publisher: SPIE
Publisher Place: Bellingham
Volume: 12
Issue: 1
Publisher DOI: 10.1117/1.1526102
Page Start: 134
Page End: 150
EISSN: 1560-229X
ISSN: 1017-9909
Notes: Revised version published in: Journal of Electronic Imaging. - 12(1), S. 134-150 (Jan 01, 2003). - doi:10.1117/1.1526102 Title: Automatic three-dimensional graph construction of nerve cells from confocal microscopy scans
Appears in Collections:Inst. Softwaretechnik und Theoretische Informatik » Publications

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