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Main Title: High-cell-density fed-batch cultivations of Vibrio natriegens
Author(s): Thiele, Isabel
Gutschmann, Björn
Aulich, Linus
Girard, Marcel
Neubauer, Peter
Riedel, Sebastian L.
Type: Article
Abstract: Objectives: With generation times of less than 10 min under optimal conditions, the halophilic Vibrio natriegens is the fastest growing non-pathogenic bacterium isolated so far. The availability of the full genome and genetic engineering tools and its ability to utilize a wide range of carbon sources make V. natriegens an attractive host for biotechnological production processes. However, high-cell-density cultivations, which are desired at industrial-scale have not been described so far. Results: In this study we report fed-batch cultivations of V. natriegens in deep-well plates and lab-scale bioreactor cultivations at different temperatures in mineral salt medium (MSM). Upon switching from exponential glucose to constant glucose-feeding cell death was induced. Initial NaCl concentrations of 15–18 g L −1 and a temperature reduction from 37 to 30 °C had a positive effect on cell growth. The maximal growth rate in MSM with glucose was 1.36 h −1 with a specific oxygen uptake rate of 22 mmol g CDW −1 h −1 . High biomass yields of up to 55 g L −1 after only 12 h were reached. Conclusions: The shown fed-batch strategies demonstrate the potential of V. natriegens as a strong producer in industrial biotechnology.
Subject(s): vibrio natriegens
deep-well plate
fast growing microorganism
high-cell-density cultivations
parallel bioreactor cultivations
Issue Date: 19-May-2021
Date Available: 6-Dec-2021
Language Code: en
DDC Class: 660 Chemische Verfahrenstechnik
Sponsor/Funder: TU Berlin, Open-Access-Mittel – 2021
Journal Title: Biotechnology Letters
Publisher: Springer Nature
Volume: 43
Issue: 9
Publisher DOI: 10.1007/s10529-021-03147-5
Page Start: 1723
Page End: 1733
EISSN: 1573-6776
ISSN: 0141-5492
TU Affiliation(s): Fak. 3 Prozesswissenschaften » Inst. Biotechnologie » FG Bioverfahrenstechnik
Appears in Collections:Technische Universität Berlin » Publications

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