High-cell-density fed-batch cultivations of Vibrio natriegens

Thiele, Isabel; Gutschmann, Björn; Aulich, Linus; Girard, Marcel; Neubauer, Peter; Riedel, Sebastian L.

FG Bioverfahrenstechnik

Objectives: With generation times of less than 10 min under optimal conditions, the halophilic Vibrio natriegens is the fastest growing non-pathogenic bacterium isolated so far. The availability of the full genome and genetic engineering tools and its ability to utilize a wide range of carbon sources make V. natriegens an attractive host for biotechnological production processes. However, high-cell-density cultivations, which are desired at industrial-scale have not been described so far. Results: In this study we report fed-batch cultivations of V. natriegens in deep-well plates and lab-scale bioreactor cultivations at different temperatures in mineral salt medium (MSM). Upon switching from exponential glucose to constant glucose-feeding cell death was induced. Initial NaCl concentrations of 15–18 g L −1 and a temperature reduction from 37 to 30 °C had a positive effect on cell growth. The maximal growth rate in MSM with glucose was 1.36 h −1 with a specific oxygen uptake rate of 22 mmol g CDW −1 h −1 . High biomass yields of up to 55 g L −1 after only 12 h were reached. Conclusions: The shown fed-batch strategies demonstrate the potential of V. natriegens as a strong producer in industrial biotechnology.