Please use this identifier to cite or link to this item:
For citation please use:
Main Title: From spores to fungal pellets: A new high-throughput image analysis highlights the structural development of Aspergillus niger
Author(s): Müller, Henri
Barthel, Lars
Schmideder, Stefan
Schütze, Tabea
Meyer, Vera
Briesen, Heiko
Type: Article
Abstract: Many filamentous fungi are exploited as cell factories in biotechnology. Cultivated under industrially relevant submerged conditions, filamentous fungi can adopt different macromorphologies ranging from dispersed mycelia over loose clumps to pellets. Central to the development of a pellet morphology is the agglomeration of spores after inoculation followed by spore germination and outgrowth into a pellet population, which is usually very heterogeneous. As the dynamics underlying population heterogeneity is not yet fully understood, we present here a new high-throughput image analysis pipeline based on stereomicroscopy to comprehensively assess the developmental program starting from germination up to pellet formation. To demonstrate the potential of this pipeline, we used data from 44 sampling times harvested during a 48 h submerged batch cultivation of the fungal cell factory Aspergillus niger. The analysis of up to 1700 spore agglomerates and 1500 pellets per sampling time allowed the precise tracking of the morphological development of the overall culture. The data gained were used to calculate size distributions and area fractions of spores, spore agglomerates, spore agglomerates within pellets, pellets, and dispersed mycelia. This approach eventually enables the quantification of culture heterogeneities and pellet breakage.
Subject(s): Aspergillus niger
image analysis
morphology development
pellet growth
spore agglomeration/aggregation
Issue Date: 9-May-2022
Date Available: 18-Jul-2022
Language Code: en
DDC Class: 570 Biowissenschaften; Biologie
Sponsor/Funder: DFG, 315384307, Verallgemeinerte morphologische Modellierung aggregierender, filamentöser Mikroorganismen
DFG, 315305620, Untersuchung des Einflusses von Scherkräften auf das morphogenetische Gennetzwerk, die Zellintegrität, mikroskopische und makroskopische Morphologie von Aspergillus niger sowie Bildungsraten intra- und extrazellulärer Produkte
DFG, 427889137, Kontrolle von Monospezies und Multispezies Pellet-Heterogenitäten und deren Auswirkungen auf Produktbildung in der Zellfabrik Aspergillus niger
Journal Title: Biotechnology and Bioengineering
Publisher: Wiley
Volume: 119
Issue: 8
Publisher DOI: 10.1002/bit.28124
Page Start: 2182
Page End: 2195
EISSN: 1097-0290
ISSN: 0006-3592
TU Affiliation(s): Fak. 3 Prozesswissenschaften » Inst. Biotechnologie » FG Angewandte und Molekulare Mikrobiologie
Appears in Collections:Technische Universität Berlin » Publications

Files in This Item:
Format: Adobe PDF | Size: 3.29 MB
DownloadShow Preview

Item Export Bar

This item is licensed under a Creative Commons License Creative Commons