Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-4508
Main Title: Genome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger reveals a coordinated cellular response to counteract ER stress
Author(s): Carvalho, Neuza
Jorgensen, Thomas
Arentshorst, Mark
Nitsche, Benjamin
van den Hondel, Cees A. M. J. J.
Archer, David
Ram, Arthur
Type: Article
Language: English
Language Code: en
Abstract: Background: HacA/Xbp1 is a conserved bZIP transcription factor in eukaryotic cells which regulates gene expression in response to various forms of secretion stress and as part of secretory cell differentiation. In the present study, we replaced the endogenous hacA gene of an Aspergillus niger strain with a gene encoding a constitutively active form of the HacA transcription factor (HacACA). The impact of constitutive HacA activity during exponential growth was explored in bioreactor controlled cultures using transcriptomic analysis to identify affected genes and processes. Results: Transcription profiles for the wild-type strain (HacAWT) and the HacACA strain were obtained using Affymetrix GeneChip analysis of three replicate batch cultures of each strain. In addition to the well known HacA targets such as the ER resident foldases and chaperones, GO enrichment analysis revealed up-regulation of genes involved in protein glycosylation, phospholipid biosynthesis, intracellular protein transport, exocytosis and protein complex assembly in the HacACA mutant. Biological processes over-represented in the down-regulated genes include those belonging to central metabolic pathways, translation and transcription. A remarkable transcriptional response in the HacACA strain was the down-regulation of the AmyR transcription factor and its target genes. Conclusions: The results indicate that the constitutive activation of the HacA leads to a coordinated regulation of the folding and secretion capacity of the cell, but with consequences on growth and fungal physiology to reduce secretion stress.
URI: urn:nbn:de:kobv:83-opus4-67698
http://depositonce.tu-berlin.de/handle/11303/4805
http://dx.doi.org/10.14279/depositonce-4508
Issue Date: 2012
Date Available: 25-Jun-2015
DDC Class: 570 Biowissenschaften; Biologie
610 Medizin und Gesundheit
Subject(s): Aspergillus niger
HacA
Protein secretion
RESS
Secretion stress
Unfolded protein response
XBP1
Creative Commons License: https://creativecommons.org/licenses/by/2.0/
Journal Title: BMC Genomics
Publisher: BioMed Central
Publisher Place: London
Volume: 13
Article Number: 350
Publisher DOI: 10.1186/1471-2164-13-350
Notes: First published by BioMed Central: Carvalho, Neuza ; Jorgensen, Thomas ; Arentshorst, Mark ; Nitsche, Benjamin ; van den Hondel, Cees ; Archer, David ; Ram, Arthur: Genome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger reveals a coordinated cellular response to counteract ER stress. - In: BMC Genomics. - ISSN 1471-2164 (online). - 13 (2012), art. 350. - doi:10.1186/1471-2164-13-350.
Appears in Collections:Technische Universität Berlin » Fakultäten & Zentralinstitute » Fakultät 3 Prozesswissenschaften » Institut für Biotechnologie » Publications

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