Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-4597
Main Title: Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1
Author(s): Niu, Ben
Vater, Joachim
Rueckert, Christian
Blom, Jochen
Lehmann, Maik
Ru, Jin-Jiang
Chen, Xiao-Hua
Wang, Qi
Borriss, Rainer
Type: Article
Language: English
Language Code: en
Abstract: Background: Nine gene clusters dedicated to nonribosomal synthesis of secondary metabolites with possible antimicrobial action, including polymyxin and fusaricidin, were detected within the whole genome sequence of the plant growth-promoting rhizobacterium (PGPR) Paenibacillus polymyxa M-1. To survey the antimicrobial compounds expressed by M-1 we analyzed the active principle suppressing phytopathogenic Erwinia spp. Results: P. polymyxa M-1 suppressed the growth of phytopathogenic Erwinia amylovora Ea 273, and E. carotovora, the causative agents of fire blight and soft rot, respectively. By MALDI-TOF mass spectrometry and reversed-phase high-performance liquid chromatography (RP-HPLC), two antibacterial compounds bearing molecular masses of 1190.9 Da and 1176.9 Da were detected as being the two components of polymyxin P, polymyxin P1 and P2, respectively. The active principle acting against the two Erwinia strains was isolated from TLC plates and identified by postsource decay (PSD)-MALDI-TOF mass spectrometry as polymyxin P1 and polymyxin P2. These findings were corroborated by domain structure analysis of the polymyxin (pmx) gene cluster detected in the M-1 chromosome which revealed that corresponding to the chemical structure of polymyxin P, the gene cluster is encoding D-Phe in position 6 and L-Thr in position 7. Conclusions: Identical morphological changes in the cell wall of the bacterial phytopathogens treated with either crude polymyxin P or culture supernatant of M-1 corroborated that polymyxin P is the main component of the biocontrol effect exerted by strain M-1 against phytopathogenic Erwinia spp.
URI: urn:nbn:de:kobv:83-opus4-69884
http://depositonce.tu-berlin.de/handle/11303/4894
http://dx.doi.org/10.14279/depositonce-4597
Issue Date: 2013
Date Available: 4-Aug-2015
DDC Class: 570 Biowissenschaften; Biologie
610 Medizin und Gesundheit
Subject(s): Antibiotics
Paenibacillus polymyxa M-1
Polymyxin
Creative Commons License: https://creativecommons.org/licenses/by/2.0/
Journal Title: BMC Microbiology
Publisher: BioMed Central
Publisher Place: London
Volume: 13
Article Number: 137
Publisher DOI: 10.1186/1471-2180-13-137
Notes: Published by BioMed Central Niu, Ben ; Vater, Joachim ; Rueckert, Christian ; Blom, Jochen ; Lehmann, Maik ; Ru, Jin-Jiang ; Chen, Xiao-Hua ; Wang, Qi ; Borriss, Rainer : Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. - In: BMC Microbiology. - ISSN 1471-2180 (online). - 13 (2013), art. 137. - doi:10.1186/1471-2180-13-137.
Appears in Collections:Technische Universität Berlin » Fakultäten & Zentralinstitute » Fakultät 2 Mathematik und Naturwissenschaften » Institut für Chemie » Publications

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