Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-4971
Main Title: GTP-binding protein Era: a novel gene target for biofuel production
Author(s): Voshol, Gerben P.
Meyer, Vera
van den Hondel, Cees A. M. J. J.
Type: Article
Language Code: en
Abstract: Background: Biodiesel production using cyanobacteria is a promising alternative to fossil fuels. In this study we created a transposon library of Synechococcus elongatus PCC 7942 in order to identify novel gene targets for enhanced fatty acid and hydrocarbon production. The transposon library was subsequently screened for desirable traits using macro- and microscopic observations as well as staining with the lipophilic dye Nile Red. Results: Based on the screening results, we selected a single mutant, which has an insertion in the gene encoding for the GTP-binding protein Era. We subsequently verified the phenotype-genotype relation by overexpression, reintroducing and complementing the mutation. Overexpression of era caused a reduction in the cell size in the late exponential phase of growth and an increase in the total amount of intracellular fatty acids. Reintroduction of the inactivated transposon caused a significant increase in the cellular length as well as changes in the amounts of individual hydrocarbons and fatty acids. Ectopic complementation of this mutation fully restored the hydrocarbon production profile to that of wild-type and partially restored the fatty acid production. Moreover, the cellular size was significantly smaller than that of the inactivated transposon mutant. Conclusions: The GTP-binding protein Era has never been studied in cyanobacteria and proved to be an essential gene for S. elongatus PCC 7942. We also found that this protein is important for hydrocarbon and fatty acid metabolism as well as determination of the cell size in PCC 7942. Our results suggest that the GTP-binding protein Era can be used as a novel target for further improvement of biofuel precursors production.
URI: http://depositonce.tu-berlin.de/handle/11303/5283
http://dx.doi.org/10.14279/depositonce-4971
Issue Date: 24-Mar-2015
Date Available: 1-Feb-2016
DDC Class: DDC::500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
DDC::600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
Subject(s): biofuels
cyanobacteria
GTP-binding protein Era
fatty acids
hydrocarbons
Synechococcus elongatus PCC 7942
Biokraftstoffe
Cyanobakterien
Hydrokarbonate
Fettsäuren
Creative Commons License: https://creativecommons.org/licenses/by/4.0/
Journal Title: BMC Biotechnology
Publisher: BioMed Central
Publisher Place: London
Volume: 15
Article Number: 21
Publisher DOI: 10.1186/s12896-015-0132-1
EISSN: 1472-6750
Appears in Collections:Technische Universität Berlin » Fakultäten & Zentralinstitute » Fakultät 3 Prozesswissenschaften » Institut für Biotechnologie » Publications

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