Inst. Biotechnologie

300 Items

Recent Submissions
Analysis of metabolic pathways controlling cAMP-regulated aquaporin-2 trafficking in renal principal cells

Schulz, Maike (2018)

One of the main effectors of the second messenger cyclic adenosine monophosphate (cAMP) is the ubiquitously expressed serine/threonine protein kinase, cAMP-dependent protein kinase (PKA). Binding of cAMP to the regulatory subunits of PKA results in a conformational change and the release of the catalytic subunits to phosphorylate nearby targets. To ensure spatially and temporally controlled pho...

Optimization of cell-laden bioinks for 3D bioprinting and efficient infection with influenza A virus

Berg, Johanna ; Hiller, Thomas ; Kissner, Maya S. ; Qazi, Taimoor H. ; Duda, Georg N. ; Hocke, Andreas C. ; Hippenstiel, Stefan ; Elomaa, Laura ; Weinhart, Marie ; Fahrenson, Christoph ; Kurreck, Jens (2018-09-17)

Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of ...

Sequencing traces for plasmid pDR111

Giessmann, Robert T. (2018)

This data object contains the raw sequencing traces generated by Sanger dideoxy sequencing for the plasmid pDR111, a kind gift of David Z. Rudner.

Development of recombinant oncolytic Coxsackievirus B3 for treatment of colorectal carcinoma

Hazini, Ahmet (2018)

Colorectal cancer is one of the most death related cancer type in worldwide. Conventional cancer therapeutics show curable effect only in the patients at early stage of the disease. In case of the diagnosis at late stages, the survival rate decreases dramatically up to 10%. Therefore, novel cancer therapeutics are urgently needed. Oncolytic virotherapy is a new treatment approach which exploits...

Rocking Aspergillus: morphology-controlled cultivation of Aspergillus niger in a wave-mixed bioreactor for the production of secondary metabolites

Kurt, Tutku ; Marbà-Ardébol, Anna-Maria ; Turan, Zeynep ; Neubauer, Peter ; Junne, Stefan ; Meyer, Vera (2018-08-21)

Background Filamentous fungi including Aspergillus niger are cell factories for the production of organic acids, proteins and bioactive compounds. Traditionally, stirred-tank reactors (STRs) are used to cultivate them under highly reproducible conditions ensuring optimum oxygen uptake and high growth rates. However, agitation via mechanical stirring causes high shear forces, thus affecting fun...

In vivo-Analyse der B-Zell-Rezeptor-Signaltransduktion im Keimzentrum

Ulbricht, Carolin (2018)

Unser Immunsystem stellt uns eine Vielzahl von Effektormechanismen zur Verfügung, die für den lebenslangen Schutz vor Krankheitserregern sorgen. Ein Aspekt dieser Mechanismen ist die Produktion von Immunglobulin-Antikörpern durch langlebige Plasmazellen, die aus B-Zellen in Keimzentren der sekundären lymphatischen Organe stammen. Innerhalb der Keimzentren bilden B-Zellen, T-Zellen und follikulä...

Reconstruction of the lantibiotic ruminococcin-A biosynthesis machinery in Escherichia coli and structural characterization

Ongey, Elvis Legala (2018)

Lanthipeptides are a group of therapeutically relevant proteinaceous compounds predominantly produced by Gram-positive bacteria at the ribosomal level, containing thioether cross-linked amino acids called lanthionine as a characteristic structural element. Lanthipeptides are attracting growing interests stemming from their wide range of bioactivities including antimicrobial, antiviral and antic...

Monitoring of the single-cell morphology for the evaluation of microbial eukaryotic bioprocesses

Marbà Ardébol, Anna Maria (2018)

Cell morphology is not only influenced by the cell cycle, the aging or individual properties, but also by environmental impacts such as those occurring on a large-scale. Cell morphology can be a suitable parameter for in situ measurements as it changes dynamically and is often related to cell physiology. In order to be able to identify relationships between cell physiology and morphology, stati...

Development of eukaryotic cell-free systems based on Chinese hamster ovary cells for the production of "difficult-to-express" proteins

Thoring, Lena (2018)

Nowadays, recombinant protein production plays a pivotal role for the manufacturing of biopharmaceuticals. A variety of in vivo production systems are available originating from eukaryotic and prokaryotic hosts, whereby Chinese Hamster Ovary cells (CHO cells) are one of the main production cell line for complex, mammalian proteins. Limitations of cell based protein production systems are often ...

Identification of hypoxia-specific anti-cancer drugs using an in vitro spheroid model

Klutzny, Saskia (2018)

Cancer is among the leading causes of morbidity and mortality worldwide and the effectiveness of novel treatment approaches is often limited by the development of drug resistance. Owing to the rapid growth, high metabolic rates and lagging or insufficient neo-angiogenesis, many solid tumors are characterized by large regions of hypoxia. Tumor hypoxia is a main determinant for resistance develop...

Heterologous biosynthesis, modifications and structural characterization of ruminococcin-A, a lanthipeptide from the gut bacterium Ruminococcus gnavus E1, in Escherichia coli

Ongey, Elvis Legala ; Giessmann, Robert ; Fons, Michel ; Rappsilber, Juri ; Adrian, Lorenz ; Neubauer, Peter (2018-07-26)

Ruminococcin A (RumA) is a lanthipeptide with high activity against pathogenic clostridia and is naturally produced by the strict anaerobic bacterium Ruminococcus gnavus E1, isolated from human intestine. Cultivating R. gnavus E1 is challenging, limiting high-quality production, further biotechnological development and therapeutic exploitation of RumA. To supply an alternative production system...

P45 IL-2 therapy reduces renal inflammation and cellular activity of intrarenal CD4+ conventional T cells in lupus prone mice with active lupus nephritis

Rose, A. ; von Spee-Mayer, C. ; Kloke, Lutz ; Wu, K. ; Enghard, P. ; Kühl, A. ; Humrich, J.Y. ; Riemekasten, G. (2016-11)

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Bioinspired designs, molecular premise and tools for evaluating the ecological importance of antimicrobial peptides

Ongey, Elvis Legala ; Pflugmacher, Stephan ; Neubauer, Peter (2018-07-10)

This review article provides an overview of recent developments in antimicrobial peptides (AMPs), summarizing structural diversity, potential new applications, activity targets and microbial killing responses in general. The use of artificial and natural AMPs as templates for rational design of peptidomimetics are also discussed and some strategies are put forward to curtail cytotoxic effects a...

Real-time monitoring of the budding index in Saccharomyces cerevisiae batch cultivations with in situ microscopy

Marbà-Ardébol, Anna-Maria ; Emmerich, Jörn ; Muthig, Michael ; Neubauer, Peter ; Junne, Stefan (2018-05-15)

Background The morphology of yeast cells changes during budding, depending on the growth rate and cultivation conditions. A photo-optical microscope was adapted and used to observe such morphological changes of individual cells directly in the cell suspension. In order to obtain statistically representative samples of the population without the influence of sampling, in situ microscopy (ISM) w...

An observational study of ballooning in large spiders: Nanoscale multifibers enable large spiders’ soaring flight

Cho, Moonsung ; Neubauer, Peter ; Fahrenson, Christoph ; Rechenberg, Ingo (2018-06-14)

The physical mechanism of aerial dispersal of spiders, “ballooning behavior,” is still unclear because of the lack of serious scientific observations and experiments. Therefore, as a first step in clarifying the phenomenon, we studied the ballooning behavior of relatively large spiders (heavier than 5 mg) in nature. Additional wind tunnel tests to identify ballooning silks were implemented in t...

Construction of an improved Aspergillus niger platform for enhanced glucoamylase secretion

Fiedler, Markus R. M. ; Barthel, Lars ; Kubisch, Christin ; Nai, Corrado ; Meyer, Vera (2018-06-16)

Background The lifestyle of filamentous fungi depends on the secretion of hydrolytic enzymes into the surrounding medium, which degrade polymeric substances into monomers that are then taken up to sustain metabolism. This feature has been exploited in biotechnology to establish platform strains with high secretory capacity including Aspergillus niger. The accepted paradigm is that proteins bec...

G9a/GLP complex maintains imprinted DNA methylation in embryonic stem cells

Zhang, Tuo ; Termanis, Ausma ; Özkan, Burak ; Bao, Xun X. ; Culley, Jayne ; de Lima Alves, Flavia ; Rappsilber, Juri ; Ramsahoye, Bernard ; Stancheva, Irina (2016-03-24)

DNA methylation at imprinting control regions (ICRs) is established in gametes in a sex-specific manner and has to be stably maintained during development and in somatic cells to ensure the correct monoallelic expression of imprinted genes. In addition to DNA methylation, the ICRs are marked by allele-specific histone modifications. Whether these marks are essential for maintenance of genomic i...

HisB as novel selection marker for gene targeting approaches in Aspergillus niger

Fiedler, Markus R. M. ; Gensheimer, Tarek ; Kubisch, Christin ; Meyer, Vera (2017-03-08)

Background For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene int...

Kicking against the PRCs

Liang, Shih Chieh ; Hartwig, Ben ; Perera, Pumi ; Mora-García, Santiago ; Leau, Erica de ; Thornton, Harry ; Lima Alves, Flavia de ; Rappsilber, Juri ; Yang, Suxin ; James, Geo Velikkakam ; Schneeberger, Korbinian ; Finnegan, E. Jean ; Turck, Franziska ; Goodrich, Justin (2015)

The Polycomb group (PcG) and trithorax group (trxG) genes play crucial roles in development by regulating expression of homeotic and other genes controlling cell fate. Both groups catalyse modifications of chromatin, particularly histone methylation, leading to epigenetic changes that affect gene activity. The trxG antagonizes the function of PcG genes by activating PcG target genes, and conseq...

Anti-adenoviral artificial microRNAs Expressed from AAV9 vectors inhibit human adenovirus infection in immunosuppressed Syrian hamsters

Schaar, Katrin ; Geisler, Anja ; Kraus, Milena ; Pinkert, Sandra ; Pryshliak, Markian ; Spencer, Jacqueline F. ; Tollefson, Ann E. ; Ying, Baoling ; Kurreck, Jens ; Wold, William S. ; Klopfleisch, Robert ; Toth, Karoly ; Fechner, Henry (2017-09)

Infections of immunocompromised patients with human adenoviruses (hAd) can develop into life-threatening conditions, whereas drugs with anti-adenoviral efficiency are not clinically approved and have limited efficacy. Small double-stranded RNAs that induce RNAi represent a new class of promising anti-adenoviral therapeutics. However, as yet, their efficiency to treat hAd5 infections has only be...