Inst. Biotechnologie

320 Items

Recent Submissions
Verbesserung des CVB3-Maus-Myokarditismodells durch die Entwicklung pankreasattenuierter Coxsackieviren

Pryshliak, Markian (2019)

Das Coxsackievirus B3 (CVB3)-Maus-Myokarditismodell wird standardmäßig zur Untersuchung der Virus-induzierten Myokarditis verwendet. Entgegen der CVB3-Infektion im Menschen stellt im murinen Organismus nicht das Herz, sondern das Pankreas das für die CVB3-Infektion suszeptibelste Organ dar. Verschiedene klinische Symptome, wie der starke Gewichtsverlust, das schmerzhafte Hochziehen des Bauches ...

Impact of TiO2 nanoparticles on the aquatic environment: Investigation of cyanobacterial toxin adsorption and oxidative stress mediated nanotoxicity towards the submerged aquatic macrophyte Hydrilla verticillata

Spengler, Annette (2019)

Manufactured TiO2-nanoparticles (TiO2-NPs) are the most commonly employed metal oxide-based NPs. Due to the greater surface area per unit volume compared with their respective microscale counterparts, TiO2-NPs might exhibit physicochemical properties which affect the toxicity potential of the normally inert material. Taking into account the increasing production volumes of TiO2-NPs, they will i...

Modeling and simulation of biogas production based on anaerobic digestion of energy crops and manure

Arzate Salgado, Juan Antonio (2019)

Despite all knowledge related to AD, the complexity of the process leads to the fact that there exists still a lack of knowledge how the process will behave at disturbances and flexible feedstock utilization. At biogas plants, sampling and monitoring is in most cases not sufficient to detect disturbances in the process at an early stage. This lack of knowledge does not allow for a demand-driven...

Analyse der Hyperinflammation im Verlauf der murinen Pneumokokken-Pneumonie unter besonderer Berücksichtigung des Neutrophile-rekrutierenden Chemokins CXCL5

Berger, Sarah (2019)

Die ambulant erworbene Pneumonie ist mit ihrer hohen Inzidenz und Mortalität eine der bedeutendsten Infektionskrankheiten weltweit. Streptococcus pneumoniae (S. pn.) ist der häufigste Erreger der ambulant erworbenen Pneumonie. Eine wichtige Ursache für die hohe Sterblichkeit trotz wirksamer antibiotischer Therapie stellt die umgreifende Schädigung eines inadäquat aktivierten Immunsystems währen...

Modelling overflow metabolism in Escherichia coli by acetate cycling

Anane, Emmanuel ; López Cárdenas, Diana Carolina ; Neubauer, Peter ; Cruz-Bournazou, M. Nicolas (2017-05-25)

A new set of mathematical equations describing overflow metabolism and acetate accumulation in E. coli cultivation is presented. The model is a significant improvement of already existing models in the literature, with modifications based on the more recent concept of acetate cycling in E. coli, as revealed by proteomic studies of overflow routes. This concept opens up new questions regarding t...

Aspergillus niger is a superior expression host for the production of bioactive fungal cyclodepsipeptides

Boecker, Simon ; Grätz, Stefan ; Kerwat, Dennis ; Adam, Lutz ; Schirmer, David ; Richter, Lennart ; Schütze, Tabea ; Petras, Daniel ; Süßmuth, Roderich D. ; Meyer, Vera (2018-03-02)

Background: Fungal cyclodepsipeptides (CDPs) are non-ribosomally synthesized peptides produced by a variety of flamentous fungi and are of interest to the pharmaceutical industry due to their anticancer, antimicrobial and anthelmintic bioactivities. However, both chemical synthesis and isolation of CDPs from their natural producers are limited due to high costs and comparatively low yields. The...

Model for tooth development in vitro

Rosowski, Jennifer (2019)

The ultimate goal of Tissue Engineering is the regeneration of whole organs or functional organ units. Recent approaches rely on the usage of adult tissue-specific cells in three-dimensional culture. It is now accepted that "Developmental Engineering", the recapitulation of critical initial steps of organogenesis in vitro, is more expedient. Identification of key players and basic mechanisms be...

Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks

Schäpe, Paul ; Kwon, Min Jin ; Baumann, Birgit ; Gutschmann, Björn ; Jung, Sascha ; Lenz, Swantje ; Nitsche, Benjamin ; Paege, Norman ; Schütze, Tabea ; Cairns, Timothy C. ; Meyer, Vera (2018)

A significant challenge in our understanding of biological systems is the high number of genes with unknown function in many genomes. The fungal genus Aspergillus contains important pathogens of humans, model organisms, and microbial cell factories. Aspergillus niger is used to produce organic acids, proteins, and is a promising source of new bioactive secondary metabolites. Out of the 14,165 o...

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Uusitalo, S. ; Kögler, Martin ; Välimaa, A.-L. ; Popov, A. ; Ryabchikov, Yu ; Kontturi, V. ; Siitonen, S. ; Petäjä, J. ; Virtanen, T. ; Laitinen, R. ; Kinnunen, M. ; Meglinski, I. ; Kabashin, A. ; Bunker, A. ; Viitala, T. ; Hiltunen, J. (2016)

The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been stu...

Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates

Knepper, Andreas ; Heiser, Michael ; Glauche, Florian ; Neubauer, Peter (2014)

The enormous variation possibilities of bioprocesses challenge process development to fix a commercial process with respect to costs and time. Although some cultivation systems and some devices for unit operations combine the latest technology on miniaturization, parallelization, and sensing, the degree of automation in upstream and downstream bioprocess development is still limited to single s...

Development of a High-Throughput Screening Assay Based on the 3-Dimensional Pannus Model for Rheumatoid Arthritis

Ibold, Yvonne ; Frauenschuh, Simone ; Kaps, Christian ; Sittinger, Michael ; Ringe, Jochen ; Goetz, Peter M. (2007)

The 3-dimensional (3-D) pannus model for rheumatoid arthritis (RA) is based on the interactive co-culture of cartilage and synovial fibroblasts (SFs). Besides the investigation of the pathogenesis of RA, it can be used to analyze the active profiles of antirheumatic pharmaceuticals and other bioactive substances under in vitro conditions. For a potential application in the industrial drug-scree...

Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus

Rudeck, Juliane (2018)

Die Verwendung einer optimalen Analgesie sollte oberste Priorität in der Versuchstierkunde haben und ist nicht nur aus ethischen, sondern auch aus rechtlichen Gründen verpflichtend. Laut nationalen und internationalen Versuchstierstatistiken ist die Maus mit ca. 70 % die am häufigsten verwendete Spezies. Opioide werden gewöhnlich zur perioperativen Behandlung von moderaten bis schweren Schmerze...

Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification of Lactobacillus plantarum Cultures

Pellicer-Alborch, Klaus ; Angersbach, Alexander ; Neubauer, Peter ; Junne, Stefan (2018-12-04)

The rapid assessment of cell viability is crucial for process optimization, e.g., during media selection, determination of optimal environmental growth conditions and for quality control. In the present study, the cells' electric anisotropy of polarizability (AP) as well as the mean cell length in Lactobacillus plantarum batch and fed-batch fermentations were monitored with electrooptical measu...

Mobile multi-parameter measurements for the dynamic analysis of gradients in brewing vessels

Bockisch, Anika (2018)

Industrial processes have to be competitive and thus efficient. Products have to be produced with a high reproducibility and quality. At the same time, the scale of these processes reaches up to several 100 m³ and was even increasing in recent years. This is challenging since the power input is limited in large-scale processes. Therefore, mixing times are increasing in parallel to the volume, w...

In silico prediction and characterization of secondary metabolite biosynthetic gene clusters in the wheat pathogen Zymoseptoria tritici

Cairns, Timothy ; Meyer, Vera (2017-08-17)

Background Fungal pathogens of plants produce diverse repertoires of secondary metabolites, which have functions ranging from iron acquisition, defense against immune perturbation, to toxic assaults on the host. The wheat pathogen Zymoseptoria tritici causes Septoria tritici blotch, a foliar disease which is a significant threat to global food security. Currently, there is limited knowledge of...

Polycistronic gene expression in Aspergillus niger

Schütze, Tabea ; Meyer, Vera (2017-09-25)

Background Genome mining approaches predict dozens of biosynthetic gene clusters in each of the filamentous fungal genomes sequenced so far. However, the majority of these gene clusters still remain cryptic because they are not expressed in their natural host. Simultaneous expression of all genes belonging to a biosynthetic pathway in a heterologous host is one approach to activate biosyntheti...

Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform

Sawatzki, Annina ; Hans, Sebastian ; Narayanan, Harini ; Haby, Benjamin ; Krausch, Niels ; Sokolov, Michael ; Glauche, Florian ; Riedel, Sebastian L. ; Neubauer, Peter ; Cruz Bournazou, Mariano Nicolas (2018-11-21)

Mini-bioreactor systems enabling automatized operation of numerous parallel cultivations are a promising alternative to accelerate and optimize bioprocess development allowing for sophisticated cultivation experiments in high throughput. These include fed-batch and continuous cultivations with multiple options of process control and sample analysis which deliver valuable screening tools for ind...

Phthalocyanine-fullerene dyads and DNA interstrand cross-linking on surface

Véliz Montes, Cinthya Yamila (2018)

Nanotechnology deals with structures and components of nanoscale size in order to create new properties or to improve significantly their physical, chemical and biological properties, processes and phenomena. Many efforts of molecular nanotechnology are directed to design artificial nanostructures on surfaces due to their potential applications in nanodevices and biosensors. This thesis include...

Studies on the Biosynthesis of γ–Decalactone in Sporobolomyces odorus

Albrecht, Wolfgang ; Tressl, Roland (1990)

Incubation of racemic ethyl [2,2-2H2]-(E)-3,4-epoxydecanoate and the corresponding acid with intact cells of Sporobolomyces odorus led to the formation of deuterium labeled γ-decalaetone. The detection of labeled 2-decen-4-olide and 4-oxodecanoic acid made it possi­ble to propose the biogenetic sequence leading to γ-decalactone. The determination of the en­ antiomeric ratio of the lactone revea...

Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies

Hiller, Thomas ; Berg, Johanna ; Elomaa, Laura ; Röhrs, Viola ; Ullah, Imran ; Schaar, Katrin ; Dietrich, Ann-Christin ; Al-Zeer, Munir A. ; Kurtz, Andreas ; Hocke, Andreas C. ; Hippenstiel, Stefan ; Fechner, Henry ; Weinhart, Marie ; Kurreck, Jens (2018-10-12)

Bioprinting is a novel technology that may help to overcome limitations associated with two-dimensional (2D) cell cultures and animal experiments, as it allows the production of three-dimensional (3D) tissue models composed of human cells. The present study describes the optimization of a bioink composed of alginate, gelatin and human extracellular matrix (hECM) to print human HepaRG liver cell...