Inst. Biotechnologie

313 Items

Recent Submissions
Model for tooth development in vitro

Rosowski, Jennifer (2019)

The ultimate goal of Tissue Engineering is the regeneration of whole organs or functional organ units. Recent approaches rely on the usage of adult tissue-specific cells in three-dimensional culture. It is now accepted that "Developmental Engineering", the recapitulation of critical initial steps of organogenesis in vitro, is more expedient. Identification of key players and basic mechanisms be...

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Uusitalo, S. ; Kögler, Martin ; Välimaa, A.-L. ; Popov, A. ; Ryabchikov, Yu ; Kontturi, V. ; Siitonen, S. ; Petäjä, J. ; Virtanen, T. ; Laitinen, R. ; Kinnunen, M. ; Meglinski, I. ; Kabashin, A. ; Bunker, A. ; Viitala, T. ; Hiltunen, J. (2016)

The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been stu...

Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates

Knepper, Andreas ; Heiser, Michael ; Glauche, Florian ; Neubauer, Peter (2014)

The enormous variation possibilities of bioprocesses challenge process development to fix a commercial process with respect to costs and time. Although some cultivation systems and some devices for unit operations combine the latest technology on miniaturization, parallelization, and sensing, the degree of automation in upstream and downstream bioprocess development is still limited to single s...

Development of a High-Throughput Screening Assay Based on the 3-Dimensional Pannus Model for Rheumatoid Arthritis

Ibold, Yvonne ; Frauenschuh, Simone ; Kaps, Christian ; Sittinger, Michael ; Ringe, Jochen ; Goetz, Peter M. (2007)

The 3-dimensional (3-D) pannus model for rheumatoid arthritis (RA) is based on the interactive co-culture of cartilage and synovial fibroblasts (SFs). Besides the investigation of the pathogenesis of RA, it can be used to analyze the active profiles of antirheumatic pharmaceuticals and other bioactive substances under in vitro conditions. For a potential application in the industrial drug-scree...

Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus

Rudeck, Juliane (2018)

Die Verwendung einer optimalen Analgesie sollte oberste Priorität in der Versuchstierkunde haben und ist nicht nur aus ethischen, sondern auch aus rechtlichen Gründen verpflichtend. Laut nationalen und internationalen Versuchstierstatistiken ist die Maus mit ca. 70 % die am häufigsten verwendete Spezies. Opioide werden gewöhnlich zur perioperativen Behandlung von moderaten bis schweren Schmerze...

Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification of Lactobacillus plantarum Cultures

Pellicer-Alborch, Klaus ; Angersbach, Alexander ; Neubauer, Peter ; Junne, Stefan (2018-12-04)

The rapid assessment of cell viability is crucial for process optimization, e.g., during media selection, determination of optimal environmental growth conditions and for quality control. In the present study, the cells' electric anisotropy of polarizability (AP) as well as the mean cell length in Lactobacillus plantarum batch and fed-batch fermentations were monitored with electrooptical measu...

Mobile multi-parameter measurements for the dynamic analysis of gradients in brewing vessels

Bockisch, Anika (2018)

Industrial processes have to be competitive and thus efficient. Products have to be produced with a high reproducibility and quality. At the same time, the scale of these processes reaches up to several 100 m³ and was even increasing in recent years. This is challenging since the power input is limited in large-scale processes. Therefore, mixing times are increasing in parallel to the volume, w...

In silico prediction and characterization of secondary metabolite biosynthetic gene clusters in the wheat pathogen Zymoseptoria tritici

Cairns, Timothy ; Meyer, Vera (2017-08-17)

Background Fungal pathogens of plants produce diverse repertoires of secondary metabolites, which have functions ranging from iron acquisition, defense against immune perturbation, to toxic assaults on the host. The wheat pathogen Zymoseptoria tritici causes Septoria tritici blotch, a foliar disease which is a significant threat to global food security. Currently, there is limited knowledge of...

Polycistronic gene expression in Aspergillus niger

Schütze, Tabea ; Meyer, Vera (2017-09-25)

Background Genome mining approaches predict dozens of biosynthetic gene clusters in each of the filamentous fungal genomes sequenced so far. However, the majority of these gene clusters still remain cryptic because they are not expressed in their natural host. Simultaneous expression of all genes belonging to a biosynthetic pathway in a heterologous host is one approach to activate biosyntheti...

Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform

Sawatzki, Annina ; Hans, Sebastian ; Narayanan, Harini ; Haby, Benjamin ; Krausch, Niels ; Sokolov, Michael ; Glauche, Florian ; Riedel, Sebastian L. ; Neubauer, Peter ; Cruz Bournazou, Mariano Nicolas (2018-11-21)

Mini-bioreactor systems enabling automatized operation of numerous parallel cultivations are a promising alternative to accelerate and optimize bioprocess development allowing for sophisticated cultivation experiments in high throughput. These include fed-batch and continuous cultivations with multiple options of process control and sample analysis which deliver valuable screening tools for ind...

Phthalocyanine-fullerene dyads and DNA interstrand cross-linking on surface

Véliz Montes, Cinthya Yamila (2018)

Nanotechnology deals with structures and components of nanoscale size in order to create new properties or to improve significantly their physical, chemical and biological properties, processes and phenomena. Many efforts of molecular nanotechnology are directed to design artificial nanostructures on surfaces due to their potential applications in nanodevices and biosensors. This thesis include...

Studies on the Biosynthesis of γ–Decalactone in Sporobolomyces odorus

Albrecht, Wolfgang ; Tressl, Roland (1990)

Incubation of racemic ethyl [2,2-2H2]-(E)-3,4-epoxydecanoate and the corresponding acid with intact cells of Sporobolomyces odorus led to the formation of deuterium labeled γ-decalaetone. The detection of labeled 2-decen-4-olide and 4-oxodecanoic acid made it possi­ble to propose the biogenetic sequence leading to γ-decalactone. The determination of the en­ antiomeric ratio of the lactone revea...

Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies

Hiller, Thomas ; Berg, Johanna ; Elomaa, Laura ; Röhrs, Viola ; Ullah, Imran ; Schaar, Katrin ; Dietrich, Ann-Christin ; Al-Zeer, Munir A. ; Kurtz, Andreas ; Hocke, Andreas C. ; Hippenstiel, Stefan ; Fechner, Henry ; Weinhart, Marie ; Kurreck, Jens (2018-10-12)

Bioprinting is a novel technology that may help to overcome limitations associated with two-dimensional (2D) cell cultures and animal experiments, as it allows the production of three-dimensional (3D) tissue models composed of human cells. The present study describes the optimization of a bioink composed of alginate, gelatin and human extracellular matrix (hECM) to print human HepaRG liver cell...

Analysis of metabolic pathways controlling cAMP-regulated aquaporin-2 trafficking in renal principal cells

Schulz, Maike (2018)

One of the main effectors of the second messenger cyclic adenosine monophosphate (cAMP) is the ubiquitously expressed serine/threonine protein kinase, cAMP-dependent protein kinase (PKA). Binding of cAMP to the regulatory subunits of PKA results in a conformational change and the release of the catalytic subunits to phosphorylate nearby targets. To ensure spatially and temporally controlled pho...

Optimization of cell-laden bioinks for 3D bioprinting and efficient infection with influenza A virus

Berg, Johanna ; Hiller, Thomas ; Kissner, Maya S. ; Qazi, Taimoor H. ; Duda, Georg N. ; Hocke, Andreas C. ; Hippenstiel, Stefan ; Elomaa, Laura ; Weinhart, Marie ; Fahrenson, Christoph ; Kurreck, Jens (2018-09-17)

Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of ...

Sequencing traces for plasmid pDR111

Giessmann, Robert T. (2018)

This data object contains the raw sequencing traces generated by Sanger dideoxy sequencing for the plasmid pDR111, a kind gift of David Z. Rudner.

Development of recombinant oncolytic Coxsackievirus B3 for treatment of colorectal carcinoma

Hazini, Ahmet (2018)

Colorectal cancer is one of the most death related cancer type in worldwide. Conventional cancer therapeutics show curable effect only in the patients at early stage of the disease. In case of the diagnosis at late stages, the survival rate decreases dramatically up to 10%. Therefore, novel cancer therapeutics are urgently needed. Oncolytic virotherapy is a new treatment approach which exploits...

Rocking Aspergillus: morphology-controlled cultivation of Aspergillus niger in a wave-mixed bioreactor for the production of secondary metabolites

Kurt, Tutku ; Marbà-Ardébol, Anna-Maria ; Turan, Zeynep ; Neubauer, Peter ; Junne, Stefan ; Meyer, Vera (2018-08-21)

Background Filamentous fungi including Aspergillus niger are cell factories for the production of organic acids, proteins and bioactive compounds. Traditionally, stirred-tank reactors (STRs) are used to cultivate them under highly reproducible conditions ensuring optimum oxygen uptake and high growth rates. However, agitation via mechanical stirring causes high shear forces, thus affecting fun...

In vivo-Analyse der B-Zell-Rezeptor-Signaltransduktion im Keimzentrum

Ulbricht, Carolin (2018)

Unser Immunsystem stellt uns eine Vielzahl von Effektormechanismen zur Verfügung, die für den lebenslangen Schutz vor Krankheitserregern sorgen. Ein Aspekt dieser Mechanismen ist die Produktion von Immunglobulin-Antikörpern durch langlebige Plasmazellen, die aus B-Zellen in Keimzentren der sekundären lymphatischen Organe stammen. Innerhalb der Keimzentren bilden B-Zellen, T-Zellen und follikulä...

Reconstruction of the lantibiotic ruminococcin-A biosynthesis machinery in Escherichia coli and structural characterization

Ongey, Elvis Legala (2018)

Lanthipeptides are a group of therapeutically relevant proteinaceous compounds predominantly produced by Gram-positive bacteria at the ribosomal level, containing thioether cross-linked amino acids called lanthionine as a characteristic structural element. Lanthipeptides are attracting growing interests stemming from their wide range of bioactivities including antimicrobial, antiviral and antic...