Inst. Biotechnologie

267 Items

Recent Submissions
Advanced Raman spectroscopy for bioprocess monitoring

Kögler, Martin (2018)

The Raman -effect was discovered almost 90 years ago. It took a long time until the importance of Raman spectroscopy was fully understood and accepted for process industrial applications. Still today the usage is limited to a small application area. During the last decades advances in the production of spectroscopic components have reduced the complexity of the instrumentation and further media...

Elucidation of orthopoxvirus adaptation mechanisms by proteomics and genomics

Grossegesse, Marica (2018)

Adaptive changes of viruses enable the virus-host coevolution and infection of new host species. While viruses with an RNA genome adapt primarily by genomic variabilities, adaptation mechanisms of viruses with a DNA genome have remained largely elusive. Since genomes of DNA viruses are more stable compared to genomes of RNA viruses, it is assumed that mechanisms other than genomic changes under...

Growth rate dependent impact of oscillating glucose conditions on Escherichia coli physiology: electronical supplement

Brand, Eva (2018)

Escherichia coli is the most used microorganism for biotechnological production processes due to its outstanding cultivation robustness and genetic and metabolic engineering possibilities. The established production process is a high cell density cultivation starting with a batch cultivation and followed by a fed-batch feeding strategy to avoid acetate accumulation and anaerobic conditions. The...

Application of the enzyme controlled glucose feeding for the production of recombinant proteins

Abdou Ali, Basant (2018)

One of the prime objectives of any bioprocess development is to enhance the microbial growth and product yields. Correspondingly, for recombinant proteins it is the aim increase the yields of the soluble, active and correctly folded molecule. There are many factors that could influence the product yield like medium optimization and the performance of the bioprocess itself. The aim of this study...

Micro-electromechanical affinity sensor for the monitoring of glucose in bioprocess media

Theuer, Lorenz ; Lehmann, Micha ; Junne, Stefan ; Neubauer, Peter ; Birkholz, Mario (2017)

An affinity-viscometry-based micro-sensor probe for continuous glucose monitoring was investigated with respect to its suitability for bioprocesses. The sensor operates with glucose and dextran competing as binding partner for concanavalin A, while the viscosity of the assay scales with glucose concentration. Changes in viscosity are determined with a micro-electromechanical system (MEMS) in th...

Platform technologies for automated bioprocess development

Glauche, Florian (2018)

The development of production processes in biotechnology is a time and resource intensive task due to the vast design space to be screened. Therefore, processes are commonly performed at a local optimum. Within the past two decades, miniaturization, parallelization and automation of laboratory work have improved significantly and researchers are now able to carry out thousands of experiments pe...

Uptake, physiological responses and technical application of the aquatic fungus Mucor hiemalis EH5 for the removal of cyanobacterial toxins

Balsano, Evelyn (2017)

Many cyanobacteria produce various harmful secondary metabolites, a.k.a. cyanotoxins, which cause global concerns because of their hazardousness to ecosystems and human health. Due to their diversity in origin and structure, cyanotoxins express different biological activities commonly including the generation of oxidative stress. This work focuses on the three most frequently occurring cyanotox...

RNA-Interferenz-basierte Therapien bei Adenovirusinfektionen im immunsupprimierten Wirt

Schaar, Katrin (2017)

Infektionen mit humanen Adenoviren verlaufen meist mild und sind nach wenigen Wochen auskuriert. Vor allem die oberen Atemwege sind betroffen, abhängig vom Serotypen können sich auch andere Symptome ergeben. Liegt eine weitere Erkrankung vor, die mit einer natürlichen oder pharmakolo-gisch induzierten Schwächung des Immunsystems einhergeht, z.B. nach Transplantationsprozeduren, verlaufen die Er...

Role of autoantigen-specific T cell subsets in disease pathogenesis and as therapeutic tool in systemic lupus erythematosus

Abdirama, Dimas (2017)

Systemic lupus erythematosus (SLE) is a chronic and systemic autoimmune disorder characterized by loss of immune tolerance towards nuclear antigens such as SmD1, RNP70, Histone, Ro, and La. Besides the production of autoantibodies that directed to nuclear antigens, CD4+ T cells with T cell receptor specific to nuclear antigens may also trigger autoimmune responses leading to severe organ inflam...

Impacts of oscillating cultivation conditions on the quality of recombinant inclusion bodies in Escherichia coli

Reitz, Christian (2017)

The controlled use of microorganisms in the pharmaceutical, (bio-)chemical and life science industries to produce recombinant proteins or other organic compounds in large-scale bioreactors is a standard procedure today. However, predicting the process performance after scaling up from the development scale to the final production scale is still a critical problem in modern bioprocess developmen...

From axenic to mixed cultures: technological advances accelerating a paradigm shift in microbiology

Meyer, Vera ; Nai, Corrado (2017)

Since the onset of microbiology in the late 19th century, scientists have been growing microorganisms almost exclusively as pure cultures, resulting in a limited and biased view of the microbial world. Only a paradigm shift in cultivation techniques – from axenic to mixed cultures – can allow a full comprehension of the (chemical) communication of microorganisms, with profound consequences for ...

Die Schädigung des unreifen Kleinhirns durch Hyperoxie in Ratten als Modell der Hirnschädigung bei Frühgeborenen

Scheuer, Till (2017)

Former preterm infants often suffer from impaired postnatal brain development. In general, brain injury is reported to be caused by hypoxia/ischemia, infection/inflammation and hyperoxia. Due to recent insights, pathologies of the cerebellum can often be found, too. Preterm birth seems to be associated with a reduction of the cerebellar volume and decreased cell density in the cerebellar cortex...

Automated universal chip platform for fluorescence based cellular assays

Schmieder, F. ; Schmieder, S. ; Eger, R. ; Friedrich, S. ; Werner, A. ; Danz, N. ; Marx, Uwe ; Sonntag, Frank (2012)

The advantage of cell based assays used as biosensors is the direct access to hardly obtainable parameters like toxicity, mutagenicity and pharmacological effectiveness. Within the last few years we established a micro fluidic platform including a peristaltic micro pump as well as several valves, manifolds and micro channels [1]. For optical online monitoring the micro fluidic system is bonded ...

Hollow fibres integrated in a microfluidic cell culture system

Winkelmann, Claudia ; Luo, Y. ; Lode, A. ; Gelinsky, M. ; Marx, Uwe ; Sonntag, Frank (2012)

For in vitro drug screening a modified perfusion micro-bioreactor system with integrated hollow fibres will be demonstrated. This biocompatible system consists of an integrated closed flow circuit that includes reservoirs and pneumatic micro pumps. Additional optical online-monitoring devices allow the observation during the cell cultivation. The embedded hollow fibre system which acts as cell ...

Automated Micro-PIV measurement in Lab-on-a-Chip systems

Busek, Mathias ; Polk, Christoph ; Albrecht, Thomas ; Marx, Uwe ; König, J. ; Sonntag, Frank (2012)

Flow rate and wall shear stress are important parameters for perfused cell culture systems and should be monitored. An easy and non-invasive method is the particle image velocimetry (PIV). In this work PIV was used to characterize a cell culture system with included peristaltic pump. The time-dependent flow profile was measured on several points of the chip for different pumping speeds to figur...

Etablierung und Charakterisierung eines dreidimensionalen Zellkulturmodells für Kuhpockeninfektionen

Koban, Robert (2017)

Zellkultivierungen stellen ein elementares Werkzeug für die Erforschung vielfältiger biologischer Phänomene dar. In der Virologie werden Infektionen in vitro fast ausschließlich in Monolayer-Kulturen charakterisiert und erforscht, in denen Zellen flach ausgebreitet auf starren Oberflächen kultiviert werden und im Vergleich zum in vivo Status der gleichen Zellen deutliche Unterschiede bezüglich ...

Studien zur endogenen Expression von RNA G-Quadruplexen und deren Interaktion mit zellulären Proteinen

Serikawa, Tatsuo (2017)

Due to specific orientation of guanine molecules certain guanine-rich sequences can form inter- or intramolecular structures, known as Guanine (G)-quadruplexes (G4s or GQs). Over the last 10 years, it has been shown several times that RNA G-quadruplexes located in the 5´ UTR of the mRNA suppress translation, however, a recent study indicated that in eukaryotic cells RNA G-quadruplexes are globa...

Etablierung eines dreidimensionalen Infektionsmodells für Kuhpockenviren in humaner Haut und Charakterisierung der Infektion

Neumann, Markus (2017)

Heutzutage stehen dreidimensionale Zellkulturmodelle für verschiedenste Anwendungen und Organe, z. B. die Haut, zur Verfügung. Für die Untersuchung viraler Infektionen bietet die Nutzung von 3D-Modellen eine wichtige physiologische Alternative, da die Organoide die Lücke zwischen Studien in klassischer 2D-Zellkultur und im Tiermodell schließen können. Im Rahmen dieser Studie werden die zoonotis...

A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents

Maschmeyer, Ilka ; Lorenz, Alexandra K. ; Schimek, Katharina ; Hasenberg, Tobias ; Ramme, Anja P. ; Hübner, Juliane ; Lindner, Marcus ; Drewell, Christopher ; Bauer, Sophie ; Thomas, Alexander ; Sambo, Naomia Sisoli ; Sonntag, Frank ; Lauster, Roland ; Marx, Uwe (2015)

Systemic absorption and metabolism of drugs in the small intestine, metabolism by the liver as well as excretion by the kidney are key determinants of efficacy and safety for therapeutic candidates. However, these systemic responses of applied substances lack in most in vitro assays. In this study, a microphysiological system maintaining the functionality of four organs over 28 days in co-cultu...

Toward microbioreactor arrays

Glauche, Florian ; John, Gernot T. ; Arain, Sarina ; Knepper, Andreas ; Neubauer, Antje ; Goelling, Detlef ; Lang, Christine ; Violet, Norman ; King, Rudibert ; Neubauer, Peter (2015)

In this study, a slow-responding chemo-optical sensor for dissolved oxygen (DO) integrated into a 96-well plate was developed. The slow response time ensures that the measured oxygen value does not change much during plate transport to the microplate reader. The sensor therefore permits at-line DO measurement of microbial cultures. Moreover, it eliminates the necessity of individual optical mea...