Inst. Biotechnologie

285 Items

Recent Submissions
Construction of an improved Aspergillus niger platform for enhanced glucoamylase secretion

Fiedler, Markus R. M. ; Barthel, Lars ; Kubisch, Christin ; Nai, Corrado ; Meyer, Vera (2018-06-16)

Background The lifestyle of filamentous fungi depends on the secretion of hydrolytic enzymes into the surrounding medium, which degrade polymeric substances into monomers that are then taken up to sustain metabolism. This feature has been exploited in biotechnology to establish platform strains with high secretory capacity including Aspergillus niger. The accepted paradigm is that proteins bec...

G9a/GLP complex maintains imprinted DNA methylation in embryonic stem cells

Zhang, Tuo ; Termanis, Ausma ; Özkan, Burak ; Bao, Xun X. ; Culley, Jayne ; de Lima Alves, Flavia ; Rappsilber, Juri ; Ramsahoye, Bernard ; Stancheva, Irina (2016-03-24)

DNA methylation at imprinting control regions (ICRs) is established in gametes in a sex-specific manner and has to be stably maintained during development and in somatic cells to ensure the correct monoallelic expression of imprinted genes. In addition to DNA methylation, the ICRs are marked by allele-specific histone modifications. Whether these marks are essential for maintenance of genomic i...

HisB as novel selection marker for gene targeting approaches in Aspergillus niger

Fiedler, Markus R. M. ; Gensheimer, Tarek ; Kubisch, Christin ; Meyer, Vera (2017-03-08)

Background For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene int...

Kicking against the PRCs

Liang, Shih Chieh ; Hartwig, Ben ; Perera, Pumi ; Mora-García, Santiago ; Leau, Erica de ; Thornton, Harry ; Lima Alves, Flavia de ; Rappsilber, Juri ; Yang, Suxin ; James, Geo Velikkakam ; Schneeberger, Korbinian ; Finnegan, E. Jean ; Turck, Franziska ; Goodrich, Justin (2015)

The Polycomb group (PcG) and trithorax group (trxG) genes play crucial roles in development by regulating expression of homeotic and other genes controlling cell fate. Both groups catalyse modifications of chromatin, particularly histone methylation, leading to epigenetic changes that affect gene activity. The trxG antagonizes the function of PcG genes by activating PcG target genes, and conseq...

Anti-adenoviral artificial microRNAs Expressed from AAV9 vectors inhibit human adenovirus infection in immunosuppressed Syrian hamsters

Schaar, Katrin ; Geisler, Anja ; Kraus, Milena ; Pinkert, Sandra ; Pryshliak, Markian ; Spencer, Jacqueline F. ; Tollefson, Ann E. ; Ying, Baoling ; Kurreck, Jens ; Wold, William S. ; Klopfleisch, Robert ; Toth, Karoly ; Fechner, Henry (2017-09)

Infections of immunocompromised patients with human adenoviruses (hAd) can develop into life-threatening conditions, whereas drugs with anti-adenoviral efficiency are not clinically approved and have limited efficacy. Small double-stranded RNAs that induce RNAi represent a new class of promising anti-adenoviral therapeutics. However, as yet, their efficiency to treat hAd5 infections has only be...

Hepatitis E virus superinfection and clinical progression in hepatitis B patients

Hoan, Nghiem Xuan ; Tong, Hoang Van ; Hecht, Nicole ; Sy, Bui Tien ; Marcinek, Patrick ; Meyer, Christian G. ; Song, Le Huu ; Toan, Nguyen Linh ; Kurreck, Jens ; Kremsner, Peter G. ; Bock, C-Thomas ; Velavan, Thirumalaisamy P. (2015)

Hepatitis E virus (HEV) infection may cause acute hepatitis and lead to hepatic failure in developing and developed countries. We studied HEV seroprevalences in patients with hepatitis B virus (HBV) infection to understand the consequences of HEV superinfection in a Vietnamese population. This cross-sectional study was conducted from 2012 to 2013 and included 1318 Vietnamese patients with HBV-r...

How a fungus shapes biotechnology: 100 years of Aspergillus niger research

Cairns, Timothy C. ; Nai, Corrado ; Meyer, Vera (2018-05-24)

In 1917, a food chemist named James Currie made a promising discovery: any strain of the filamentous mould Aspergillus niger would produce high concentrations of citric acid when grown in sugar medium. This tricarboxylic acid, which we now know is an intermediate of the Krebs cycle, had previously been extracted from citrus fruits for applications in food and beverage production. Two years afte...

Conditional expression of the small GTPase ArfA impacts secretion, morphology, growth, and actin ring position in Aspergillus niger

Fiedler, Markus R. M. ; Cairns, Timothy C. ; Koch, Oliver ; Kubisch, Christin ; Meyer, Vera (2018-05-08)

In filamentous fungi, growth and protein secretion occurs predominantly at the tip of long, thread like cells termed hyphae. This requires coordinated regulation of multiple processes, including vesicle trafficking, exocytosis, and endocytosis, which are facilitated by a complex cytoskeletal apparatus. In this study, functional analyses of the small GTPase ArfA from Aspergillus niger demonstrat...

Chip-based human liver-intestine and liver-skin co-culture

Maschmeyer, Ilka ; Hasenberg, Tobias ; Jaenicke, Annika ; Lindner, Marcus ; Lorenz, Alexandra Katharina ; Zech, Julie ; Garbe, Leif-Alexander ; Sonntag, Frank ; Hayden, Patrick ; Ayehunie, Seyoum ; Lauster, Roland ; Marx, Uwe ; Materne, Eva-Maria (2015)

Systemic repeated dose safety assessment and systemic efficacy evaluation of substances are currently carried out on laboratory animals and in humans due to the lack of predictive alternatives. Relevant international regulations, such as OECD and ICH guidelines, demand long-term testing and oral, dermal, inhalation, and systemic exposure routes for such evaluations. So-called “human-on-a-chip” ...

A single α helix drives extensive remodeling of the proteasome lid and completion of regulatory particle assembly

Tomko, Robert J. ; Taylor, David W. ; Chen, Zhuo A. ; Wang, Hong-Wei ; Rappsilber, Juri ; Hochstrasser, Mark (2015)

Most short-lived eukaryotic proteins are degraded by the proteasome. A proteolytic core particle (CP) capped by regulatory particles (RPs) constitutes the 26S proteasome complex. RP biogenesis culminates with the joining of two large subcomplexes, the lid and base. In yeast and mammals, the lid appears to assemble completely before attaching to the base, but how this hierarchical assembly is en...

The Aspergillus giganteus antifungal protein AFPNN5353activates the cell wall integrity pathway and perturbs calcium homeostasis

Binder, Ulrike ; Bencina, Mojca ; Eigentler, Andrea ; Meyer, Vera ; Marx, Florentine (2011-09-23)

Background The antifungal protein AFPNN5353 is a defensin-like protein of Aspergillus giganteus. It belongs to a group of secretory proteins with low molecular mass, cationic character and a high content of cysteine residues. The protein inhibits the germination and growth of filamentous ascomycetes, including important human and plant pathogens and the model organsims Aspergillus nidulans and...

Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii

Schmid, Jochen ; Müller-Hagen, Dirk ; Bekel, Thomas ; Funk, Laura ; Stahl, Ulf ; Sieber, Volker ; Meyer, Vera (2010-05-26)

Background The plant pathogenic basidiomycete Sclerotium rolfsii produces the industrially exploited exopolysaccharide scleroglucan, a polymer that consists of (1 → 3)-β-linked glucose with a (1 → 6)-β-glycosyl branch on every third unit. Although the physicochemical properties of scleroglucan are well understood, almost nothing is known about the genetics of scleroglucan biosynthesis. Similar...

The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans

dos Reis, Thaila Fernanda ; Nitsche, Benjamin M. ; de Lima, Pollyne Borborema Almeida ; de Assis, Leandro José ; Mellado, Laura ; Harris, Steven D. ; Meyer, Vera ; dos Santos, Renato A. Corrêa ; Riaño-Pachón, Diego M. ; Ries, Laure Nicolas Annick ; Goldman, Gustavo H. (2017-03-31)

One of the drawbacks during second-generation biofuel production from plant lignocellulosic biomass is the accumulation of glucose, the preferred carbon source of microorganisms, which causes the repression of hydrolytic enzyme secretion by industrially relevant filamentous fungi. Glucose sensing, subsequent transport and cellular signalling pathways have been barely elucidated in these organis...

Growth rate dependent impact of oscillating glucose conditions on Escherichia coli physiology

Brand, Eva (2018)

Escherichia coli is the most used microorganism for biotechnological production processes due to its outstanding cultivation robustness and genetic and metabolic engineering possibilities. The established production process is a high cell density cultivation starting with a batch cultivation and followed by a fed-batch feeding strategy to avoid acetate accumulation and anaerobic conditions. The...

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Uusitalo, S. ; Kögler, Martin ; Välimaa, A.-L. ; Popov, A. ; Ryabchikov, Yu. ; Kontturi, V. ; Siitonen, S. ; Petäjä, J. ; Virtanen, T. ; Laitinen, R. ; Kinnunen, M. ; Meglinski, I. ; Kabashin, A. ; Bunker, A. ; Viitala, T. ; Hiltunen, J. (2016)

The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been stu...

Functional inhibition of acid sphingomyelinase by Fluphenazine triggers hypoxia-specific tumor cell death

Klutzny, Saskia ; Lesche, Ralf ; Keck, Matthias ; Kaulfuss, Stefan ; Schlicker, Andreas ; Christian, Sven ; Sperl, Carolyn ; Neuhaus, Roland ; Mowat, Jeffrey ; Steckel, Michael ; Riefke, Björn ; Prechtl, Stefan ; Parczyk, Karsten ; Steigemann, Patrick (2017)

Owing to lagging or insufficient neo-angiogenesis, hypoxia is a feature of most solid tumors. Hypoxic tumor regions contribute to resistance against antiproliferative chemotherapeutics, radiotherapy and immunotherapy. Targeting cells in hypoxic tumor areas is therefore an important strategy for cancer treatment. Most approaches for targeting hypoxic cells focus on the inhibition of hypoxia adap...

Emulating the human vasculature in a Multi-Organ-Chip platform

Hasenberg, Tobias (2018)

The Multi-Organ-Chip platform is a microphysiological system developed to evaluate toxicity and efficacy of drugs, and adverse effects of cosmetics, chemicals and alike in a sub-systemic mode. At the scale of a microscope glass slide, it comprises several compartments for the co-cultivation of human 3D tissue constructs. The organoids are physically separated, yet, interconnected through perfus...

Optimizing human Treg stability and target specificity for therapeutic applications

Nowak, Anna (2018)

Regulatory T cells (Tregs) are essential mediators of tolerance and crucial for the containment of inflammatory immune reactions. Their immunosuppressive potential is currently investigated for the treatment of inflammatory diseases. However, conversion between the Treg and conventional T cell (Tcon) compartment has been described, comprising the induction or loss of the regulatory phenotype in...

Advanced Raman spectroscopy for bioprocess monitoring

Kögler, Martin (2018)

The Raman -effect was discovered almost 90 years ago. It took a long time until the importance of Raman spectroscopy was fully understood and accepted for process industrial applications. Still today the usage is limited to a small application area. During the last decades advances in the production of spectroscopic components have reduced the complexity of the instrumentation and further media...

Elucidation of orthopoxvirus adaptation mechanisms by proteomics and genomics

Grossegesse, Marica (2018)

Adaptive changes of viruses enable the virus-host coevolution and infection of new host species. While viruses with an RNA genome adapt primarily by genomic variabilities, adaptation mechanisms of viruses with a DNA genome have remained largely elusive. Since genomes of DNA viruses are more stable compared to genomes of RNA viruses, it is assumed that mechanisms other than genomic changes under...