Inst. Biotechnologie

327 Items

Recent Submissions
Monte Carlo Simulations for the Analysis of Non-linear Parameter Confidence Intervals in Optimal Experimental Design

Krausch, Niels ; Barz, Tilman ; Sawatzki, Annina ; Gruber, Mathis ; Kamel, Sarah ; Neubauer, Peter ; Cruz Bournazou, Mariano Nicolas (2019-05-24)

Especially in biomanufacturing, methods to design optimal experiments are a valuable technique to fully exploit the potential of the emerging technical possibilities that are driving experimental miniaturization and parallelization. The general objective is to reduce the experimental effort while maximizing the information content of an experiment, speeding up knowledge gain in R&D. The approac...

Dynamic Modelling of Phosphorolytic Cleavage Catalyzed by Pyrimidine-Nucleoside Phosphorylase

Giessmann, Robert T. ; Krausch, Niels ; Kaspar, Felix ; Cruz Bournazou, Mariano Nicolas ; Wagner, Anke ; Neubauer, Peter ; Gimpel, Matthias (2019-06-19)

Pyrimidine-nucleoside phosphorylases (Py-NPases) have a significant potential to contribute to the economic and ecological production of modified nucleosides. These can be produced via pentose-1-phosphates, an interesting but mostly labile and expensive precursor. Thus far, no dynamic model exists for the production process of pentose-1-phosphates, which involves the equilibrium state of the Py...

Emulating the early phases of human tooth development in vitro

Rosowski, Jennifer ; Bräunig, Julia ; Amler, Anna-Klara ; Strietzel, Frank P. ; Lauster, Roland ; Rosowski, Mark (2019-05-07)

Functional in vitro models emulating the physiological processes of human organ formation are invaluable for future research and the development of regenerative therapies. Here, a developmentally inspired approach is pursued to reproduce fundamental steps of human tooth organogenesis in vitro using human dental pulp cells. Similar to the in vivo situation of tooth initiating mesenchymal condens...

3-Iodothyronamine and its role in aminergic G-protein coupled receptor signaling and neuromodulation

Bräunig, Julia (2019)

3-Iodothyronamine (T1AM) is an endogenous thyroid hormone metabolite. Its profound pharmacological effects on energy metabolism and thermal homeostasis in rodents have raised interest to elucidate its signaling properties in tissues that pertain to metabolic regulation and thermogenesis. Especially its anapyrexic effect could be utilized in emergency medicine to treat disease conditions like st...

Model-based strategies for scale-down studies in fed-batch cultivation of Escherichia coli expressing proinsulin

Anane, Emmanuel (2019)

A fundamental discrepancy in process performance (yields) exists between laboratory and industrial scale fermentation processes. This discrepancy results from heterogeneous environments in large-scale bioreactors due to the longer mixing times associated with the increasing volumes at industrial scale, compared to laboratory scale bioreactors. The effects of such process inhomogeneity or the so...

Merging science and art through fungi

Meyer, Vera (2019-04-26)

Science and art have long been studied interchangeably, with notable polymaths emerging in the Renaissance such as Leonardo da Vinci (artist, inventor, engineer and anatomist) and Alexander von Humboldt (explorer, geographer and naturalist) with his fellow investigators Johann Wolfgang von Goethe (scientist and writer) and Friedrich Schiller (philosopher, physician and historian). However, this...

CFD-basierte evolutions­strategische Black-Box-Optimierung zur Abschätzung des Potentials des Bionikpropellers im realen Einsatz am Schiff

Schmid, Michael (2019)

Die Evolution hat in der Natur viele Lösungen unter dem besonderen Gesichtspunkt der Energieeffizienz hervorgebracht. Dabei sind insbesondere bei Vögeln interessante Lösungen entstanden, die sich im Sinne der Bionik als effizienzsteigernde Maßnamen verstehen und technisch umsetzen lassen. Der bei der Auftriebserzeugung am Vogel- bzw. Tragflügel entstehende Spitzenwirbel ist unvermeidlich, der E...

Verbesserung des CVB3-Maus-Myokarditismodells durch die Entwicklung pankreasattenuierter Coxsackieviren

Pryshliak, Markian (2019)

Das Coxsackievirus B3 (CVB3)-Maus-Myokarditismodell wird standardmäßig zur Untersuchung der Virus-induzierten Myokarditis verwendet. Entgegen der CVB3-Infektion im Menschen stellt im murinen Organismus nicht das Herz, sondern das Pankreas das für die CVB3-Infektion suszeptibelste Organ dar. Verschiedene klinische Symptome, wie der starke Gewichtsverlust, das schmerzhafte Hochziehen des Bauches ...

Impact of TiO2 nanoparticles on the aquatic environment: Investigation of cyanobacterial toxin adsorption and oxidative stress mediated nanotoxicity towards the submerged aquatic macrophyte Hydrilla verticillata

Spengler, Annette (2019)

Manufactured TiO2-nanoparticles (TiO2-NPs) are the most commonly employed metal oxide-based NPs. Due to the greater surface area per unit volume compared with their respective microscale counterparts, TiO2-NPs might exhibit physicochemical properties which affect the toxicity potential of the normally inert material. Taking into account the increasing production volumes of TiO2-NPs, they will i...

Modeling and simulation of biogas production based on anaerobic digestion of energy crops and manure

Arzate Salgado, Juan Antonio (2019)

Despite all knowledge related to AD, the complexity of the process leads to the fact that there exists still a lack of knowledge how the process will behave at disturbances and flexible feedstock utilization. At biogas plants, sampling and monitoring is in most cases not sufficient to detect disturbances in the process at an early stage. This lack of knowledge does not allow for a demand-driven...

Analyse der Hyperinflammation im Verlauf der murinen Pneumokokken-Pneumonie unter besonderer Berücksichtigung des Neutrophile-rekrutierenden Chemokins CXCL5

Berger, Sarah (2019)

Die ambulant erworbene Pneumonie ist mit ihrer hohen Inzidenz und Mortalität eine der bedeutendsten Infektionskrankheiten weltweit. Streptococcus pneumoniae (S. pn.) ist der häufigste Erreger der ambulant erworbenen Pneumonie. Eine wichtige Ursache für die hohe Sterblichkeit trotz wirksamer antibiotischer Therapie stellt die umgreifende Schädigung eines inadäquat aktivierten Immunsystems währen...

Modelling overflow metabolism in Escherichia coli by acetate cycling

Anane, Emmanuel ; López Cárdenas, Diana Carolina ; Neubauer, Peter ; Cruz-Bournazou, M. Nicolas (2017-05-25)

A new set of mathematical equations describing overflow metabolism and acetate accumulation in E. coli cultivation is presented. The model is a significant improvement of already existing models in the literature, with modifications based on the more recent concept of acetate cycling in E. coli, as revealed by proteomic studies of overflow routes. This concept opens up new questions regarding t...

Aspergillus niger is a superior expression host for the production of bioactive fungal cyclodepsipeptides

Boecker, Simon ; Grätz, Stefan ; Kerwat, Dennis ; Adam, Lutz ; Schirmer, David ; Richter, Lennart ; Schütze, Tabea ; Petras, Daniel ; Süßmuth, Roderich D. ; Meyer, Vera (2018-03-02)

Background: Fungal cyclodepsipeptides (CDPs) are non-ribosomally synthesized peptides produced by a variety of flamentous fungi and are of interest to the pharmaceutical industry due to their anticancer, antimicrobial and anthelmintic bioactivities. However, both chemical synthesis and isolation of CDPs from their natural producers are limited due to high costs and comparatively low yields. The...

Model for tooth development in vitro

Rosowski, Jennifer (2019)

The ultimate goal of Tissue Engineering is the regeneration of whole organs or functional organ units. Recent approaches rely on the usage of adult tissue-specific cells in three-dimensional culture. It is now accepted that "Developmental Engineering", the recapitulation of critical initial steps of organogenesis in vitro, is more expedient. Identification of key players and basic mechanisms be...

Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks

Schäpe, Paul ; Kwon, Min Jin ; Baumann, Birgit ; Gutschmann, Björn ; Jung, Sascha ; Lenz, Swantje ; Nitsche, Benjamin ; Paege, Norman ; Schütze, Tabea ; Cairns, Timothy C. ; Meyer, Vera (2018)

A significant challenge in our understanding of biological systems is the high number of genes with unknown function in many genomes. The fungal genus Aspergillus contains important pathogens of humans, model organisms, and microbial cell factories. Aspergillus niger is used to produce organic acids, proteins, and is a promising source of new bioactive secondary metabolites. Out of the 14,165 o...

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Uusitalo, S. ; Kögler, Martin ; Välimaa, A.-L. ; Popov, A. ; Ryabchikov, Yu ; Kontturi, V. ; Siitonen, S. ; Petäjä, J. ; Virtanen, T. ; Laitinen, R. ; Kinnunen, M. ; Meglinski, I. ; Kabashin, A. ; Bunker, A. ; Viitala, T. ; Hiltunen, J. (2016)

The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been stu...

Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates

Knepper, Andreas ; Heiser, Michael ; Glauche, Florian ; Neubauer, Peter (2014)

The enormous variation possibilities of bioprocesses challenge process development to fix a commercial process with respect to costs and time. Although some cultivation systems and some devices for unit operations combine the latest technology on miniaturization, parallelization, and sensing, the degree of automation in upstream and downstream bioprocess development is still limited to single s...

Development of a High-Throughput Screening Assay Based on the 3-Dimensional Pannus Model for Rheumatoid Arthritis

Ibold, Yvonne ; Frauenschuh, Simone ; Kaps, Christian ; Sittinger, Michael ; Ringe, Jochen ; Goetz, Peter M. (2007)

The 3-dimensional (3-D) pannus model for rheumatoid arthritis (RA) is based on the interactive co-culture of cartilage and synovial fibroblasts (SFs). Besides the investigation of the pathogenesis of RA, it can be used to analyze the active profiles of antirheumatic pharmaceuticals and other bioactive substances under in vitro conditions. For a potential application in the industrial drug-scree...

Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus

Rudeck, Juliane (2018)

Die Verwendung einer optimalen Analgesie sollte oberste Priorität in der Versuchstierkunde haben und ist nicht nur aus ethischen, sondern auch aus rechtlichen Gründen verpflichtend. Laut nationalen und internationalen Versuchstierstatistiken ist die Maus mit ca. 70 % die am häufigsten verwendete Spezies. Opioide werden gewöhnlich zur perioperativen Behandlung von moderaten bis schweren Schmerze...

Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification of Lactobacillus plantarum Cultures

Pellicer-Alborch, Klaus ; Angersbach, Alexander ; Neubauer, Peter ; Junne, Stefan (2018-12-04)

The rapid assessment of cell viability is crucial for process optimization, e.g., during media selection, determination of optimal environmental growth conditions and for quality control. In the present study, the cells' electric anisotropy of polarizability (AP) as well as the mean cell length in Lactobacillus plantarum batch and fed-batch fermentations were monitored with electrooptical measu...