Inst. Biotechnologie

307 Items

Recent Submissions
Mobile multi-parameter measurements for the dynamic analysis of gradients in brewing vessels

Bockisch, Anika (2018)

Industrial processes have to be competitive and thus efficient. Products have to be produced with a high reproducibility and quality. At the same time, the scale of these processes reaches up to several 100 m³ and was even increasing in recent years. This is challenging since the power input is limited in large-scale processes. Therefore, mixing times are increasing in parallel to the volume, w...

In silico prediction and characterization of secondary metabolite biosynthetic gene clusters in the wheat pathogen Zymoseptoria tritici

Cairns, Timothy ; Meyer, Vera (2017-08-17)

Background Fungal pathogens of plants produce diverse repertoires of secondary metabolites, which have functions ranging from iron acquisition, defense against immune perturbation, to toxic assaults on the host. The wheat pathogen Zymoseptoria tritici causes Septoria tritici blotch, a foliar disease which is a significant threat to global food security. Currently, there is limited knowledge of...

Polycistronic gene expression in Aspergillus niger

Schütze, Tabea ; Meyer, Vera (2017-09-25)

Background Genome mining approaches predict dozens of biosynthetic gene clusters in each of the filamentous fungal genomes sequenced so far. However, the majority of these gene clusters still remain cryptic because they are not expressed in their natural host. Simultaneous expression of all genes belonging to a biosynthetic pathway in a heterologous host is one approach to activate biosyntheti...

Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform

Sawatzki, Annina ; Hans, Sebastian ; Narayanan, Harini ; Haby, Benjamin ; Krausch, Niels ; Sokolov, Michael ; Glauche, Florian ; Riedel, Sebastian L. ; Neubauer, Peter ; Cruz Bournazou, Mariano Nicolas (2018-11-21)

Mini-bioreactor systems enabling automatized operation of numerous parallel cultivations are a promising alternative to accelerate and optimize bioprocess development allowing for sophisticated cultivation experiments in high throughput. These include fed-batch and continuous cultivations with multiple options of process control and sample analysis which deliver valuable screening tools for ind...

Phthalocyanine-fullerene dyads and DNA interstrand cross-linking on surface

Véliz Montes, Cinthya Yamila (2018)

Nanotechnology deals with structures and components of nanoscale size in order to create new properties or to improve significantly their physical, chemical and biological properties, processes and phenomena. Many efforts of molecular nanotechnology are directed to design artificial nanostructures on surfaces due to their potential applications in nanodevices and biosensors. This thesis include...

Studies on the Biosynthesis of γ–Decalactone in Sporobolomyces odorus

Albrecht, Wolfgang ; Tressl, Roland (1990)

Incubation of racemic ethyl [2,2-2H2]-(E)-3,4-epoxydecanoate and the corresponding acid with intact cells of Sporobolomyces odorus led to the formation of deuterium labeled γ-decalaetone. The detection of labeled 2-decen-4-olide and 4-oxodecanoic acid made it possi­ble to propose the biogenetic sequence leading to γ-decalactone. The determination of the en­ antiomeric ratio of the lactone revea...

Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies

Hiller, Thomas ; Berg, Johanna ; Elomaa, Laura ; Röhrs, Viola ; Ullah, Imran ; Schaar, Katrin ; Dietrich, Ann-Christin ; Al-Zeer, Munir A. ; Kurtz, Andreas ; Hocke, Andreas C. ; Hippenstiel, Stefan ; Fechner, Henry ; Weinhart, Marie ; Kurreck, Jens (2018-10-12)

Bioprinting is a novel technology that may help to overcome limitations associated with two-dimensional (2D) cell cultures and animal experiments, as it allows the production of three-dimensional (3D) tissue models composed of human cells. The present study describes the optimization of a bioink composed of alginate, gelatin and human extracellular matrix (hECM) to print human HepaRG liver cell...

Analysis of metabolic pathways controlling cAMP-regulated aquaporin-2 trafficking in renal principal cells

Schulz, Maike (2018)

One of the main effectors of the second messenger cyclic adenosine monophosphate (cAMP) is the ubiquitously expressed serine/threonine protein kinase, cAMP-dependent protein kinase (PKA). Binding of cAMP to the regulatory subunits of PKA results in a conformational change and the release of the catalytic subunits to phosphorylate nearby targets. To ensure spatially and temporally controlled pho...

Optimization of cell-laden bioinks for 3D bioprinting and efficient infection with influenza A virus

Berg, Johanna ; Hiller, Thomas ; Kissner, Maya S. ; Qazi, Taimoor H. ; Duda, Georg N. ; Hocke, Andreas C. ; Hippenstiel, Stefan ; Elomaa, Laura ; Weinhart, Marie ; Fahrenson, Christoph ; Kurreck, Jens (2018-09-17)

Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of ...

Sequencing traces for plasmid pDR111

Giessmann, Robert T. (2018)

This data object contains the raw sequencing traces generated by Sanger dideoxy sequencing for the plasmid pDR111, a kind gift of David Z. Rudner.

Development of recombinant oncolytic Coxsackievirus B3 for treatment of colorectal carcinoma

Hazini, Ahmet (2018)

Colorectal cancer is one of the most death related cancer type in worldwide. Conventional cancer therapeutics show curable effect only in the patients at early stage of the disease. In case of the diagnosis at late stages, the survival rate decreases dramatically up to 10%. Therefore, novel cancer therapeutics are urgently needed. Oncolytic virotherapy is a new treatment approach which exploits...

Rocking Aspergillus: morphology-controlled cultivation of Aspergillus niger in a wave-mixed bioreactor for the production of secondary metabolites

Kurt, Tutku ; Marbà-Ardébol, Anna-Maria ; Turan, Zeynep ; Neubauer, Peter ; Junne, Stefan ; Meyer, Vera (2018-08-21)

Background Filamentous fungi including Aspergillus niger are cell factories for the production of organic acids, proteins and bioactive compounds. Traditionally, stirred-tank reactors (STRs) are used to cultivate them under highly reproducible conditions ensuring optimum oxygen uptake and high growth rates. However, agitation via mechanical stirring causes high shear forces, thus affecting fun...

In vivo-Analyse der B-Zell-Rezeptor-Signaltransduktion im Keimzentrum

Ulbricht, Carolin (2018)

Unser Immunsystem stellt uns eine Vielzahl von Effektormechanismen zur Verfügung, die für den lebenslangen Schutz vor Krankheitserregern sorgen. Ein Aspekt dieser Mechanismen ist die Produktion von Immunglobulin-Antikörpern durch langlebige Plasmazellen, die aus B-Zellen in Keimzentren der sekundären lymphatischen Organe stammen. Innerhalb der Keimzentren bilden B-Zellen, T-Zellen und follikulä...

Reconstruction of the lantibiotic ruminococcin-A biosynthesis machinery in Escherichia coli and structural characterization

Ongey, Elvis Legala (2018)

Lanthipeptides are a group of therapeutically relevant proteinaceous compounds predominantly produced by Gram-positive bacteria at the ribosomal level, containing thioether cross-linked amino acids called lanthionine as a characteristic structural element. Lanthipeptides are attracting growing interests stemming from their wide range of bioactivities including antimicrobial, antiviral and antic...

Monitoring of the single-cell morphology for the evaluation of microbial eukaryotic bioprocesses

Marbà Ardébol, Anna Maria (2018)

Cell morphology is not only influenced by the cell cycle, the aging or individual properties, but also by environmental impacts such as those occurring on a large-scale. Cell morphology can be a suitable parameter for in situ measurements as it changes dynamically and is often related to cell physiology. In order to be able to identify relationships between cell physiology and morphology, stati...

Development of eukaryotic cell-free systems based on Chinese hamster ovary cells for the production of "difficult-to-express" proteins

Thoring, Lena (2018)

Nowadays, recombinant protein production plays a pivotal role for the manufacturing of biopharmaceuticals. A variety of in vivo production systems are available originating from eukaryotic and prokaryotic hosts, whereby Chinese Hamster Ovary cells (CHO cells) are one of the main production cell line for complex, mammalian proteins. Limitations of cell based protein production systems are often ...

Identification of hypoxia-specific anti-cancer drugs using an in vitro spheroid model

Klutzny, Saskia (2018)

Cancer is among the leading causes of morbidity and mortality worldwide and the effectiveness of novel treatment approaches is often limited by the development of drug resistance. Owing to the rapid growth, high metabolic rates and lagging or insufficient neo-angiogenesis, many solid tumors are characterized by large regions of hypoxia. Tumor hypoxia is a main determinant for resistance develop...

Heterologous biosynthesis, modifications and structural characterization of ruminococcin-A, a lanthipeptide from the gut bacterium Ruminococcus gnavus E1, in Escherichia coli

Ongey, Elvis Legala ; Giessmann, Robert ; Fons, Michel ; Rappsilber, Juri ; Adrian, Lorenz ; Neubauer, Peter (2018-07-26)

Ruminococcin A (RumA) is a lanthipeptide with high activity against pathogenic clostridia and is naturally produced by the strict anaerobic bacterium Ruminococcus gnavus E1, isolated from human intestine. Cultivating R. gnavus E1 is challenging, limiting high-quality production, further biotechnological development and therapeutic exploitation of RumA. To supply an alternative production system...

P45 IL-2 therapy reduces renal inflammation and cellular activity of intrarenal CD4+ conventional T cells in lupus prone mice with active lupus nephritis

Rose, A. ; von Spee-Mayer, C. ; Kloke, Lutz ; Wu, K. ; Enghard, P. ; Kühl, A. ; Humrich, J.Y. ; Riemekasten, G. (2016-11)

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Bioinspired designs, molecular premise and tools for evaluating the ecological importance of antimicrobial peptides

Ongey, Elvis Legala ; Pflugmacher, Stephan ; Neubauer, Peter (2018-07-10)

This review article provides an overview of recent developments in antimicrobial peptides (AMPs), summarizing structural diversity, potential new applications, activity targets and microbial killing responses in general. The use of artificial and natural AMPs as templates for rational design of peptidomimetics are also discussed and some strategies are put forward to curtail cytotoxic effects a...