Inst. Biotechnologie

345 Items

Recent Submissions
The calcineurin inhibitor cyclosporine A activates the renal Na-(K)-Cl cotransporters via local and systemic mechanisms

Blankenstein, Katharina Ilse (2019)

The two calcineurin inhibitors (CNIs) cyclosporine A (CsA) and tacrolimus are potent immunosuppressive drugs that have become indispensable in the post-transplantational therapy and in the treatment of autoimmune diseases. Immunosuppression via CNIs is achieved by inhibition of the calcineurin phosphatase and thus blocking the signaling pathway of NFAT (nuclear factor of activated T cells), the...

Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications

Schimek, Katharina ; Hsu, Hao-Hsiang ; Boehme, Moritz ; Kornet, Jacob Jan ; Marx, Uwe ; Lauster, Roland ; Pörtner, Ralf ; Lindner, Gerd (2018-06-07)

The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attracti...

Antifungal Peptides of the AFP Family Revisited: Are These Cannibal Toxins?

Meyer, Vera ; Jung, Sascha (2018-06-02)

The emergence and spread of pathogenic fungi resistant to currently used antifungal drugs represents a serious challenge for medicine and agriculture. The use of smart antimicrobials, so-called “dirty drugs” which affect multiple cellular targets, is one strategy to prevent resistance. Of special interest is the exploitation of the AFP family of antimicrobial peptides, which include its foundin...

Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Peebo, Karl ; Neubauer, Peter (2018-06-21)

Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines the output of a recombinant protein producing system, having control over specific growth rate o...

Chemoenzymatic synthesis of nucleoside analogs as potential medicinal agents

Mohamed, Heba (2019)

Modified nucleosides are important drugs used to treat cancer, viral or bacterial infections. They also serve as precursors for the synthesis of modified oligonucleotides (antisense oligonucleotides (ASOs) or short interfering RNAs (siRNAs)), a novel and effective class of therapeutics. While the chemical synthesis of nucleoside analogs is challenging due to multi-step procedures and low select...

Downstream processing of lactic acid fermentation broth from renewable feed stocks

Hendrik, Laube (2019)

Der Schwerpunkt dieser Arbeit liegt auf der Aufreinigung von Milchsäure aus Fermentationslösungen als Ausgangsstoff für die Polymerchemie. Dabei kommt der chemischen Prozessanalyse als Ausgangspunkt für die Evaluierung der jeweiligen Effektivität des Prozessschrittes eine wichtige Rolle zu. Es wurde eine Kapillarelektrophorese-Methode mittels UV/VIS-Detektor entwickelt, welche neben Milchsäure ...

Moulding the mould: understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories

Cairns, Timothy C. ; Zheng, Xiaomei ; Zheng, Ping ; Sun, Jibin ; Meyer, Vera (2019-04-02)

Filamentous fungi are harnessed as cell factories for the production of a diverse range of organic acids, proteins, and secondary metabolites. Growth and morphology have critical implications for product titres in both submerged and solid-state fermentations. Recent advances in systems-level understanding of the filamentous lifestyle and development of sophisticated synthetic biological tools f...

Is Ghana Ready to Attain Sustainable Development Goal (SDG) Number 7?—A Comprehensive Assessment of Its Renewable Energy Potential and Pitfalls

Acheampong, Michael ; Yu, Qiuyan ; Cansu Ertem, Funda ; Deba Enomah Ebude, Lucy ; Tanim, Shakhawat ; Eduful, Michael ; Vaziri, Mehrdad ; Ananga, Erick (2019-01-28)

Ghana has declared support for the UN Sustainable Development Goal (SDG) number seven which most importantly target ensuring universal access to affordable, reliable and modern energy services. This target presents a formidable challenge to Ghana because the country still relies mainly on traditional biomass as its primary source of energy coupled with a chronically fragile hydropower sector. I...

Life cycle assessment and modeling approaches as a combined evaluation tool for sustainable control strategies at biogas plants

Ertem-Kappler, Funda Cansu (2019)

Anaerobic digestion and biogas production can help to meet several targets concerning energy and environmental policy. However, biogas generation is a complex process where many challenges need to be tackled to achieve real sustainable operation. In this regard, better control, monitoring and optimization strategies need to be implemented. This thesis focuses on the combination of Life Cycle As...

Quantitative and sensitive RNA based detection of Bacillus spores

Osmekhina, Ekaterina ; Shvetsova, Antonina ; Ruottinen, Maria ; Neubauer, Peter (2014-03-11)

The fast and reliable detection of bacterial spores is of great importance and still remains a challenge. Here we describe a direct RNA-based diagnostic method for the specific detection of viable bacterial spores which does not depends on an enzymatic amplification step and therefore is directly appropriate for quantification. The procedure includes the following steps: (i) heat activation of ...

Engineering Blood and Lymphatic Microvascular Networks in Fibrin Matrices

Knezevic, Lea ; Schaupper, Mira ; Mühleder, Severin ; Schimek, Katharina ; Hasenberg, Tobias ; Marx, Uwe ; Priglinger, Eleni ; Redl, Heinz ; Holnthoner, Wolfgang (2017-04-18)

Vascular network engineering is essential for nutrient delivery to tissue-engineered constructs and, consequently, their survival. In addition, the functionality of tissues also depends on tissue drainage and immune cell accessibility, which are the main functions of the lymphatic system. Engineering both the blood and lymphatic microvasculature would advance the survival and functionality of t...

A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

Kaspar, Felix ; Giessmann, Robert T. ; Krausch, Niels ; Neubauer, Peter ; Wagner, Anke ; Gimpel, Matthias (2019-07-15)

Efficient reaction monitoring is crucial for data acquisition in kinetic and mechanistic studies. However, for conversions of nucleosides to their corresponding nucleobases, as observed in enzymatically catalyzed nucleoside phosphorylation reactions, the current analytical arsenal does not meet modern requirements regarding cost, speed of analysis and high throughput. Herein, we present a UV/Vi...

Reproduction of Large-Scale Bioreactor Conditions on Microfluidic Chips

Ho, Phuong ; Westerwalbesloh, Christoph ; Kaganovitch, Eugen ; Grünberger, Alexander ; Neubauer, Peter ; Kohlheyer, Dietrich ; Lieres, Eric von (2019-04-19)

Microbial cells in industrial large-scale bioreactors are exposed to fluctuating conditions, e.g., nutrient concentration, dissolved oxygen, temperature, and pH. These inhomogeneities can influence the cell physiology and metabolism, e.g., decelerate cell growth and product formation. Microfluidic systems offer new opportunities to study such effects in great detail by examining responses to va...

3-Iodothyronamine Activates a Set of Membrane Proteins in Murine Hypothalamic Cell Lines

Bräunig, Julia ; Mergler, Stefan ; Jyrch, Sabine ; Hoefig, Carolin S. ; Rosowski, Mark ; Mittag, Jens ; Biebermann, Heike ; Khajavi, Noushafarin (2018-09-11)

3-Iodothyronamine (3-T1AM) is an endogenous thyroid hormone metabolite. The profound pharmacological effects of 3-T1AM on energy metabolism and thermal homeostasis have raised interest to elucidate its signaling properties in tissues that pertain to metabolic regulation and thermogenesis. Previous studies identified G protein-coupled receptors (GPCRs) and transient receptor potential channels (...

Bioprinting Perfusion-Enabled Liver Equivalents for Advanced Organ-on-a-Chip Applications

Grix, Tobias ; Ruppelt, Alicia ; Thomas, Alexander ; Amler, Anna-Klara ; Noichl, Benjamin P. ; Lauster, Roland ; Kloke, Lutz (2018-03-22)

Many tissue models have been developed to mimic liver-specific functions for metabolic and toxin conversion in in vitro assays. Most models represent a 2D environment rather than a complex 3D structure similar to native tissue. To overcome this issue, spheroid cultures have become the gold standard in tissue engineering. Unfortunately, spheroids are limited in size due to diffusion barriers in ...

Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates

Hans, Sebastian ; Gimpel, Matthias ; Glauche, Florian ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2018-06-25)

Metabolic engineering and genome editing strategies often lead to large strain libraries of a bacterial host. Nevertheless, the generation of competent cells is the basis for transformation and subsequent screening of these strains. While preparation of competent cells is a standard procedure in flask cultivations, parallelization becomes a challenging task when working with larger libraries an...

Altered bone formation response to mechanical loading in a mouse model of the progeroid disorder gerodermia osteodysplastica

Thelen, Michael (2019)

Bone tissue has to constantly adapt to changing mechanical requirements. The mechanisms behind mechanosensation, mechanotransduction, signal transmission and the resulting response of bone tissue are, until this day not fully understood. A failure of these processes leads to inappropriate bone architecture, bone mass and reduced mechanical stability as seen in age-related osteoporosis or rare d...

Protokoll zur Viromanalyse bei Infektionen unklarer Genese

Klenner, Jeanette (2019)

Next Generation Sequencing (NGS) eröffnete eine Vielzahl neuer Forschungsfelder in der Medizin und der Biologie. Der Einsatz von NGS-Technologien, wie z.B. der Metagenomik, reicht heute von der Grundlagenforschung bis hin zur angewandten Forschung. Allgemein wird davon ausgegangen, dass die Technologie des NGS schon in naher Zukunft in der klinischen Routinediagnostik eingesetzt werden wird. Me...

Monte Carlo Simulations for the Analysis of Non-linear Parameter Confidence Intervals in Optimal Experimental Design

Krausch, Niels ; Barz, Tilman ; Sawatzki, Annina ; Gruber, Mathis ; Kamel, Sarah ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2019-05-24)

Especially in biomanufacturing, methods to design optimal experiments are a valuable technique to fully exploit the potential of the emerging technical possibilities that are driving experimental miniaturization and parallelization. The general objective is to reduce the experimental effort while maximizing the information content of an experiment, speeding up knowledge gain in R&D. The approac...

Dynamic Modelling of Phosphorolytic Cleavage Catalyzed by Pyrimidine-Nucleoside Phosphorylase

Giessmann, Robert T. ; Krausch, Niels ; Kaspar, Felix ; Cruz-Bournazou, Mariano Nicolas ; Wagner, Anke ; Neubauer, Peter ; Gimpel, Matthias (2019-06-19)

Pyrimidine-nucleoside phosphorylases (Py-NPases) have a significant potential to contribute to the economic and ecological production of modified nucleosides. These can be produced via pentose-1-phosphates, an interesting but mostly labile and expensive precursor. Thus far, no dynamic model exists for the production process of pentose-1-phosphates, which involves the equilibrium state of the Py...