Institut für Biotechnologie

251 Items

Recent Submissions
Studien zur endogenen Expression von RNA G-Quadruplexen und deren Interaktion mit zellulären Proteinen

Serikawa, Tatsuo (2017)

Due to specific orientation of guanine molecules certain guanine-rich sequences can form inter- or intramolecular structures, known as Guanine (G)-quadruplexes (G4s or GQs). Over the last 10 years, it has been shown several times that RNA G-quadruplexes located in the 5´ UTR of the mRNA suppress translation, however, a recent study indicated that in eukaryotic cells RNA G-quadruplexes are globa...

Etablierung eines dreidimensionalen Infektionsmodells für Kuhpockenviren in humaner Haut und Charakterisierung der Infektion

Neumann, Markus (2017)

Heutzutage stehen dreidimensionale Zellkulturmodelle für verschiedenste Anwendungen und Organe, z. B. die Haut, zur Verfügung. Für die Untersuchung viraler Infektionen bietet die Nutzung von 3D-Modellen eine wichtige physiologische Alternative, da die Organoide die Lücke zwischen Studien in klassischer 2D-Zellkultur und im Tiermodell schließen können. Im Rahmen dieser Studie werden die zoonotis...

A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents

Maschmeyer, Ilka ; Lorenz, Alexandra K. ; Schimek, Katharina ; Hasenberg, Tobias ; Ramme, Anja P. ; Hübner, Juliane ; Lindner, Marcus ; Drewell, Christopher ; Bauer, Sophie ; Thomas, Alexander ; Sambo, Naomia Sisoli ; Sonntag, Frank ; Lauster, Roland ; Marx, Uwe (2015)

Systemic absorption and metabolism of drugs in the small intestine, metabolism by the liver as well as excretion by the kidney are key determinants of efficacy and safety for therapeutic candidates. However, these systemic responses of applied substances lack in most in vitro assays. In this study, a microphysiological system maintaining the functionality of four organs over 28 days in co-cultu...

Toward microbioreactor arrays

Glauche, Florian ; John, Gernot T. ; Arain, Sarina ; Knepper, Andreas ; Neubauer, Antje ; Goelling, Detlef ; Lang, Christine ; Violet, Norman ; King, Rudibert ; Neubauer, Peter (2015)

In this study, a slow-responding chemo-optical sensor for dissolved oxygen (DO) integrated into a 96-well plate was developed. The slow response time ensures that the measured oxygen value does not change much during plate transport to the microplate reader. The sensor therefore permits at-line DO measurement of microbial cultures. Moreover, it eliminates the necessity of individual optical mea...

Southern promises: a snapshot of the microbiology research landscape in South America based on bibliometric data

Nai, Corrado (2017-09)

Scientists have a single currency for productivity and impact: published articles. In an effort to map the global research landscape in microbiology, and to obviate the current lack of bibliometric analysis in the field, FEMS—the Federation of European Microbiological Societies—generated a dataset encompassing an exhaustive, worldwide list of microbiology studies for 2013–14, which further incl...

Ex vivo Charakterisierung von humanen Autoantigen-spezifischen CD4+ T-Zellen mit Fokus auf Multiple Sklerose

Gamradt, Stefanie (2017)

Trotz intensiver Forschung ist unklar, ob Myelin-reaktive CD4+ T-Zellen in der Pathogenese der Multiplen Sklerose (MS) eine relevante Rolle spielen. Ein Grund dafür ist das Fehlen einer Methode für ihre direkte Analyse. In dieser Arbeit ist es mit Hilfe der magnetischen Anreicherung von spezifisch-aktivierten CD154+ konventionellen und CD137+ regulato-rischen T-Zellen in Kombination mit multipa...

Anaerobic transformation of brominated aromatic compounds by Dehalococcoides mccartyi strain CBDB1

Yang, Chao (2017)

Brominated flame retardants are widely used compounds for fire safety in our daily life. Many of them have been reported to be toxic to humans and identified as environmental contaminants. Dehalococcoides mccartyi strains are well known for their dependence on organohalide respiration as an energy conserving process and have therefore been intensively studied. So far, much less investigations w...

Impact of oscillations in substrate and oxygen availability on Corynebacterium glutamicum cultivations

Lemoine, Anja Irene (2017)

Corynebacterium glutamicum is a microorganism which is highly relevant for industrial large scale applications. Nowadays, it is mainly used for amino acid production in the food and feed industry in a scale of several hundred cubic meters. Furthermore, it is a promising candidate for the production of bulk chemicals such as the biopolymer precursor Cadaverine. Gradient formation occurs in indus...

Die Induktion von Interleukin-10 in humanen T-Helfer-Zellen und seine transkriptionelle Regulation

Ahlers, Jonas (2017)

Die Produktion des anti-inflammatorischen Zytokins IL-10 von Th-Zellen ist ein wesentlicher Mechanismus zur Regulation von Immunantworten. Hierbei können professionelle regulatorische T-Zellen suppressive Funktionen übernehmen oder IL-10-produzierende Th-Zellen sich selber regulieren. In dieser Arbeit konnte gezeigt werden, dass die Stimulation mit den Zytokinen IFN-α, IL-6 und IL-21 über den...

The induction and function of the transcription factor c-myc in macrophages during pneumococcal pneumonia and legionellosis

Kabus, Christin Sophia (2017)

For infectious diseases, effective defense mechanisms which constitute an efficient innate host response are the keys to eliminate invading pathogens. According to different studies, the pathogenicity of bacteria is due to different virulence factors that contribute to overcome those mechanisms. Against that, the immune system evolved a large repertoire to fight the pathogen. Nevertheless, an o...

Application and de novo design of artificial gene switches for Aspergillus niger

Schütze, Tabea (2017)

Aspergillus niger ist ein filamentöser Pilz, der industriell für die Produktion von organischen Säuren und Proteinen genutzt wird. In den letzten Jahren konnte darüber hinaus die Produktion von homo- und heterologen Sekundärmetaboliten gezeigt werden. Obwohl sich A. niger genetisch verändern lässt, gibt es nur wenig induzierbare Genexpressionssysteme. Außerdem ist die Ko-Expression von mehreren...

Proteomics of a fuzzy organelle: interphase chromatin

Kustatscher, Georg ; Hégarat, Nadia ; Wills, Karen L. H. ; Furlan, Cristina ; Bukowski-Wills, Jimi-Carlo ; Hochegger, Helfrid ; Rappsilber, Juri (2014)

Chromatin proteins mediate replication, regulate expression, and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle, it defies classical organellar proteomics and canno...

Blind testing of cross-linking/mass spectrometry hybrid methods in CASP11

Schneider, Michael ; Belsom, Adam ; Rappsilber, Juri ; Brock, Oliver (2016)

Hybrid approaches combine computational methods with experimental data. The information contained in the experimental data can be leveraged to probe the structure of proteins otherwise elusive to computational methods. Compared with computational methods, the structures produced by hybrid methods exhibit some degree of experimental validation. In spite of these advantages, most hybrid methods h...

Multiclassifier combinatorial proteomics of organelle shadows at the example of mitochondria in chromatin data

Kustatscher, Georg ; Grabowski, Piotr ; Rappsilber, Juri (2016)

Subcellular localization is an important aspect of protein function, but the protein composition of many intracellular compartments is poorly characterized. For example, many nuclear bodies are challenging to isolate biochemically and thus remain inaccessible to proteomics. Here, we explore covariation in proteomics data as an alternative route to subcellular proteomes. Rather than targeting a ...

Transposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution

Davis, Matthew P. ; Carrieri, Claudia ; Saini, Harpreet K. ; van Dongen, Stijn ; Leonardi, Tommaso ; Bussotti, Giovanni ; Monahan, Jack M. ; Auchynnikava, Tania ; Bitetti, Angelo ; Rappsilber, Juri ; Allshire, Robin C. ; Shkumatava, Alena ; O'Carroll, Dónal ; Enright, Anton J. (2017)

Spermatogenesis is associated with major and unique changes to chromosomes and chromatin. Here, we sought to understand the impact of these changes on spermatogenic transcriptomes. We show that long terminal repeats (LTRs) of specific mouse endogenous retroviruses (ERVs) drive the expression of many long non-coding transcripts (lncRNA). This process occurs post-mitotically predominantly in sper...

Role of NOD2 and S100A8/S100A9 in the pathogenesis of Coxsackievirus B3-induced myocarditis

Müller, Irene (2017)

Cardiac inflammation plays a crucial role in Coxsackievirus B3 (CVB3)-induced myocarditis and is a consequence of viral-related cardiomyocyte injury, which activates the innate immune system. The nucleotide binding oligomerization domain 2 (NOD2), a cytoplasmatic pattern-recognition receptor (PRR), is responsible for the recognition of single stranded (ss) RNA. The alarmins S100A8 and S100A9 ar...

Autoantikörper-induzierte Effekte des Angiotensin II-Rezeptors Typ 1 und des Endothelinrezeptors Typ A auf humane periphere mononukleäre Zellen und ihre Relevanz bei der Pathogenese der systemischen Sklerose

Günther, Jeannine (2017)

Functional autoantibodies (aab) against the angiotensin II receptor type 1 (AT1R) and the endothelin 1 receptor type A (ETAR) have been identified in patients suffering from systemic sclerosis (SSc). Aim of this study was to investigate the effects of these aab on peripheral blood mononuclear cells (PBMCs). As a first approach, expression of AT1R and ETAR has been analysed in PBMCs of SSc-Patie...

Partielle Charakterisierung der neuartigen humanen Polyomaviren HPyV9 und HPyV12

Korup-Schulz, Sarah-Verena (2017)

Im letzten Jahrzehnt wurden elf neue humane Polyomaviren (PyV) und zahlreiche tierische PyV identifiziert. Die Charakteristika dieser PyV wie Gewebetropismus, zelluläre Eintritts- und Infektionsmechanismen sind bislang ungeklärt. Humanes PyV 9 (HPyV9), im Serum eines Nierentransplantat-Empfängers entdeckt, und Humanes PyV 12 (HPyV12), in Proben von kolorektalen Lebermetastasen identifiziert, si...

Synthetic biomaterial microenvironments to modulate paracrine effects of mesenchymal stromal cells for skeletal muscle regeneration

Qazi, Taimoor Hasan (2017)

While skeletal muscles can recover from minor injuries, severe trauma can often induce irreversible structural damage such as extensive scar tissue formation and damaged muscle fibers. This leads to functional deficits and loss in the quality of life of patients. Effective treatment of severe muscle injuries is currently an unmet clinical need. In such cases, a therapeutic intervention such as ...

Tissue engineered cartilage: how hard can it be?

Kadler, Shirin (2017)

Für die Weiterentwicklung regenerativer Therapien sind humane in vitro Modelle aus Primärzellen entscheidend. Die Isolation und Expansion primärer Zellen zieht den Verlust der Gewebsspezifität nach sich. Diese Dedifferenzierung ist in allen Zellen des Mesenchyms zu finden und ist charakterisiert durch den Verlust der Expression von Gewebsmarkern und einer spindelförmigen Zellform durch Plastika...