Inst. Biotechnologie

401 Items

Recent Submissions
Powering biocatalytical reactions with hydrogen

Al-Shameri, Ammar (2020)

H2 -driven cofactor regeneration is cheap, 100% atom-efficient, and has been applied in various biotechnological approaches both in vivo and in vitro. To date, H2-driven cofactor regeneration has been applied only in biocatalytic reactions involving NADH. H2-driven cofactor regeneration has also never been employed in multi-enzymatic cascades or for other cofactors besides NADH. In this work,...

Biochemical characterization of the organohalide respiration complex in Dehalococcoides mccartyi strain CBDB1

Seidel, Katja (2020)

Halogenated organic compounds of anthropogenic origin are often classified as environmental pollutants due to their strong persistence. However, microbial degradation or transformation has been described for many of the anthropogenic organohalides and biotechnological processes have been developed to use the responsible organisms for bioremediation at contaminated field sites. Bacteria of the c...

Effects of 5-aza-2´-deoxycytidine on primary human chondrocytes from osteoarthritic patients

Kadler, Shirin ; Vural, Özlem ; Rosowski, Jennifer ; Reiners-Schramm, Luzia ; Lauster, Roland ; Rosowski, Mark (2020-06-23)

Chondrocytes, comparable to many cells from the connective tissue, dedifferentiate and end up in a similar fibroblastoid cell type, marked by the loss of the specific expression pattern. Here, chondrocytes isolated from osteoarthritic (OA) patients were investigated. The OA chondrocytes used in this work were not affected by the loss of specific gene expression upon cell culture. The mRNA level...

The dynamic proteome of Influenza A virus infection identifies species- and strain-specific control of M segment RNA splicing

Bogdanow, Boris (2020)

All viruses hijack host cells to replicate. To this end, they have to interact with cellular factors at all stages of the replication cycle. Since these host cell factors differ markedly between species, viruses typically need to adapt in order to cross a species barrier. Advances in high-throughput sequencing have provided insights into the extraordinary diversity of viruses and genomic determ...

Comparative genomics of the aconidial Aspergillus niger strain LDM3 predicts genes associated with its high protein secretion capacity

Sui, Yu-Fei ; Ouyang, Li-Ming ; Schütze, Tabea ; Cheng, Shu ; Meyer, Vera ; Zhuang, Ying-Ping (2020-02-03)

Aspergillus niger is widely used as a cell factory for homologous and heterologous protein production. As previous studies reported that reduced sporulation favors protein secretion in A. niger, in this study, we conducted a comparative genomic analysis of the non-sporulating industrially exploited A. niger strain LDM3 in China and the reference protein secretion strain CBS 513.88 to predict th...

Extracellular matrix- and pluripotent stem cell-based tissue engineering of the kidney

Fischer, Iris (2020)

Complex, three-dimensional (3D) organ models are novel biotechnological tools for research on regeneration and disease mechanisms as well as drug development. An organ model of the kidney is urgently needed to break through decades of stagnation in the development of new treatment methods for patients with chronic kidney disease and to reduce the high failure rate of drugs in clinical tests due...

Monitoring Parallel Robotic Cultivations with Online Multivariate Analysis

Hans, Sebastian ; Ulmer, Christian ; Narayanan, Harini ; Brautaset, Trygve ; Krausch, Niels ; Neubauer, Peter ; Schäffl, Irmgard ; Sokolov, Michael ; Cruz-Bournazou, Mariano Nicolas (2020-05-14)

In conditional microbial screening, a limited number of candidate strains are tested at different conditions searching for the optimal operation strategy in production (e.g., temperature and pH shifts, media composition as well as feeding and induction strategies). To achieve this, cultivation volumes of >10 mL and advanced control schemes are required to allow appropriate sampling and analyses...

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR

Nejati, Fatemeh ; Junne, Stefan ; Kurreck, Jens ; Neubauer, Peter (2020-06-16)

Kefir grains are complex microbial systems of several groups of microorganisms. The identification and quantification of the microbial composition of milk kefirs was described in several studies, which provided an insight into the microbial consortia in this complex ecosystem. Nevertheless, the current methods for identification and quantification are not appropriate for deeper studies on kefir...

Decoding the metabolic program of deregulated MYCN expression in neuroblastoma

Arlt, Birte (2020)

The oncogene MYCN is amplified in approximately 22% of all neuroblastomas and correlates with aggressive disease and unfavorable patient survival. Directly targeting MYCN remains a challenge due to its biochemical structure. We aimed for a deeper understanding of key metabolic pathways associated to amplified MYCN to discover druggable nodes that can be directly targeted for high-risk neuroblas...

Multi-omics analysis of Aspergillus niger glucoamylase producing strains and exploration of NADPH metabolic engineering

Sui, Yufei (2020)

The filamentous fungus Aspergillus niger is one of the main cell factories used nowadays in the industry for homologous or heterologous protein production due to its extraordinary ability for protein expression and secretion. Systematic comprehension of its metabolic regulation characteristics during the fermentation process is of considerable significance to improve the performance of this ver...

Alginate-based hydrogels with patterned biophysical and biochemical cues

Lückgen, Aline (2020)

Alginate-based hydrogels have found extensive attention in the biomaterials community due to their capacity for facile chemical modification, their nontoxicity towards encapsulated cells, and their provocation of a minimal immune response upon implantation. Covalent crosslinking of this polysaccharide allows a higher degree of control over the initial and long-term mechanics compared to the tra...

Identifizierung einer Rezeptorbindestelle im Hüllprotein des Humanen Endogenen Retrovirus K(HML-2) und weitergehende Analysen der Interaktion des Hüllproteins mit zellulären Rezeptoren

Wamara, Jula (2020)

HERV-K(HML-2) ist das jüngste und am besten erhaltene humane endogene Retrovirus. Menschliche Zellen, die diese Viren alle im Genom tragen sind in der Lage funktionelle virale Hüllproteine zu exprimieren. Diese Proteine können andere Retroviren pseudotypisieren und ihren Zelltropismus dadurch verändern. Wichtige Charakteristika des Eintrittprozesses, der durch das Hüllprotein von HERV-K(HML-2) ...

Quantitative crosslinking mass spectrometry

Müller, Fränze (2020)

The dynamics of protein structures are essential for cellular processes but are difficult to monitor by existing technologies. Crosslinking mass spectrometry (CLMS) can provide residue-resolution distance restraints, which may in principle be quantified to obtain unique insights into the structural flexibility of proteins. However, quantitative crosslinking mass spectrometry (QCLMS) needs to be...

Ansätze zur Optimierung der Glutathionausbeute in der Hefe Saccharomyces spp.

Lorenz, Eric (2020-02-05)

Glutathione is the most abundant non-protein thiol compound and tripeptide found in eukaryotes. The primary function of glutathione is to maintain the redox status and to protect the cell against oxidation caused by production of e.g. radicals or environmental influences. It is subject of different studies due to its physiological importance and industrial relevance. Based on its anti-oxidative...

Separation, Characterization, and Handling of Microalgae by Dielectrophoresis

Abt, Vinzenz ; Gringel, Fabian ; Han, Arum ; Neubauer, Peter ; Birkholz, Mario (2020-04-09)

Microalgae biotechnology has a high potential for sustainable bioproduction of diverse high-value biomolecules. Some of the main bottlenecks in cell-based bioproduction, and more specifically in microalgae-based bioproduction, are due to insufficient methods for rapid and efficient cell characterization, which contributes to having only a few industrially established microalgal species in comme...

Interspecies interplay between cyanobacteria and green algae - the first step to know why cyanobacteria produce what we call “Toxins”

Omidi, Azam (2020)

Over the past decades, cyanobacteria have attracted the researcher’s attention due to their ability to produce cyanotoxins. Among cyanotoxins, microcystins (MCs) are the most commonly studied. However, their significance for the producing species beyond their toxicity is still mysterious (was reviewed in Paper I). In the present study, the interactions between a common bloom-forming cyanobacter...

Simulation of the point-mass-loaded filament in the homogeneous turbulence

Cho, Moonsung (2020-01-14)

The passive dynamics of a ballooning spider in the chaotic flow motion of atmosphere is important to understand its suspension capability. To model a ballooning silk that is suspended in the air flow, we introduce the bead-spring model. Each bead is connected to the other with a tensile and a bending spring. By adjusting each stiffness value, we can simulate various extensibility and flexibilit...

Visualisation of hairpin vortex packets in the near-ground atmospheric boundary layer during daytime

Cho, Moonsung (2020-01-14)

Visualisation of the flow structure near the ground is important to understand aerial locomotion of a passive flying animal. The video shows the visualized structures of several hairpin vortex packets in the near ground atmospheric boundary layer during daytime. The hairpin vortex packets appear as a wedge-like shape. The structure develops upwards, as it drifts in the downwind direction. (Mean...

A crab spider’s ballooning process

Cho, Moonsung (2020-01-14)

A crab spider’s ballooning process is recorded in the field condition on an artificial dome. The video is converted to negative video to visualize ballooning lines. The video contains initial phase of spinning ballooning lines, fluttering of a bundle of ballooning lines by turbulent wind flows, take-off moment and the scene of airborne state of a ballooning spider.

Suspension of a point-mass-loaded filament in non-uniform flows: The ballooning flight of spiders

Cho, Moonsung (2020)

Some spiders can disperse through the air using their fine silk filaments. This is a technique referred to as ‘ballooning’. Not only from an ecological point of view, but also from the perspective of physics, spiders’ ballooning is an interesting phenomenon, as it relates to the question of how a large particle can disperse long distances through a fluctuating fluid medium (in this case, the Ea...