Inst. Biotechnologie

292 Items

Recent Submissions
Development of eukaryotic cell-free systems based on Chinese hamster ovary cells for the production of "difficult-to-express" proteins

Thoring, Lena (2018)

Nowadays, recombinant protein production plays a pivotal role for the manufacturing of biopharmaceuticals. A variety of in vivo production systems are available originating from eukaryotic and prokaryotic hosts, whereby Chinese Hamster Ovary cells (CHO cells) are one of the main production cell line for complex, mammalian proteins. Limitations of cell based protein production systems are often ...

Identification of hypoxia-specific anti-cancer drugs using an in vitro spheroid model

Klutzny, Saskia (2018)

Cancer is among the leading causes of morbidity and mortality worldwide and the effectiveness of novel treatment approaches is often limited by the development of drug resistance. Owing to the rapid growth, high metabolic rates and lagging or insufficient neo-angiogenesis, many solid tumors are characterized by large regions of hypoxia. Tumor hypoxia is a main determinant for resistance develop...

Heterologous biosynthesis, modifications and structural characterization of ruminococcin-A, a lanthipeptide from the gut bacterium Ruminococcus gnavus E1, in Escherichia coli

Ongey, Elvis Legala ; Giessmann, Robert ; Fons, Michel ; Rappsilber, Juri ; Adrian, Lorenz ; Neubauer, Peter (2018-07-26)

Ruminococcin A (RumA) is a lanthipeptide with high activity against pathogenic clostridia and is naturally produced by the strict anaerobic bacterium Ruminococcus gnavus E1, isolated from human intestine. Cultivating R. gnavus E1 is challenging, limiting high-quality production, further biotechnological development and therapeutic exploitation of RumA. To supply an alternative production system...

P45 IL-2 therapy reduces renal inflammation and cellular activity of intrarenal CD4+ conventional T cells in lupus prone mice with active lupus nephritis

Rose, A. ; von Spee-Mayer, C. ; Kloke, Lutz ; Wu, K. ; Enghard, P. ; Kühl, A. ; Humrich, J.Y. ; Riemekasten, G. (2016-11)

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Bioinspired designs, molecular premise and tools for evaluating the ecological importance of antimicrobial peptides

Ongey, Elvis Legala ; Pflugmacher, Stephan ; Neubauer, Peter (2018-07-10)

This review article provides an overview of recent developments in antimicrobial peptides (AMPs), summarizing structural diversity, potential new applications, activity targets and microbial killing responses in general. The use of artificial and natural AMPs as templates for rational design of peptidomimetics are also discussed and some strategies are put forward to curtail cytotoxic effects a...

Real-time monitoring of the budding index in Saccharomyces cerevisiae batch cultivations with in situ microscopy

Marbà-Ardébol, Anna-Maria ; Emmerich, Jörn ; Muthig, Michael ; Neubauer, Peter ; Junne, Stefan (2018-05-15)

Background The morphology of yeast cells changes during budding, depending on the growth rate and cultivation conditions. A photo-optical microscope was adapted and used to observe such morphological changes of individual cells directly in the cell suspension. In order to obtain statistically representative samples of the population without the influence of sampling, in situ microscopy (ISM) w...

An observational study of ballooning in large spiders: Nanoscale multifibers enable large spiders’ soaring flight

Cho, Moonsung ; Neubauer, Peter ; Fahrenson, Christoph ; Rechenberg, Ingo (2018-06-14)

The physical mechanism of aerial dispersal of spiders, “ballooning behavior,” is still unclear because of the lack of serious scientific observations and experiments. Therefore, as a first step in clarifying the phenomenon, we studied the ballooning behavior of relatively large spiders (heavier than 5 mg) in nature. Additional wind tunnel tests to identify ballooning silks were implemented in t...

Construction of an improved Aspergillus niger platform for enhanced glucoamylase secretion

Fiedler, Markus R. M. ; Barthel, Lars ; Kubisch, Christin ; Nai, Corrado ; Meyer, Vera (2018-06-16)

Background The lifestyle of filamentous fungi depends on the secretion of hydrolytic enzymes into the surrounding medium, which degrade polymeric substances into monomers that are then taken up to sustain metabolism. This feature has been exploited in biotechnology to establish platform strains with high secretory capacity including Aspergillus niger. The accepted paradigm is that proteins bec...

G9a/GLP complex maintains imprinted DNA methylation in embryonic stem cells

Zhang, Tuo ; Termanis, Ausma ; Özkan, Burak ; Bao, Xun X. ; Culley, Jayne ; de Lima Alves, Flavia ; Rappsilber, Juri ; Ramsahoye, Bernard ; Stancheva, Irina (2016-03-24)

DNA methylation at imprinting control regions (ICRs) is established in gametes in a sex-specific manner and has to be stably maintained during development and in somatic cells to ensure the correct monoallelic expression of imprinted genes. In addition to DNA methylation, the ICRs are marked by allele-specific histone modifications. Whether these marks are essential for maintenance of genomic i...

HisB as novel selection marker for gene targeting approaches in Aspergillus niger

Fiedler, Markus R. M. ; Gensheimer, Tarek ; Kubisch, Christin ; Meyer, Vera (2017-03-08)

Background For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene int...

Kicking against the PRCs

Liang, Shih Chieh ; Hartwig, Ben ; Perera, Pumi ; Mora-García, Santiago ; Leau, Erica de ; Thornton, Harry ; Lima Alves, Flavia de ; Rappsilber, Juri ; Yang, Suxin ; James, Geo Velikkakam ; Schneeberger, Korbinian ; Finnegan, E. Jean ; Turck, Franziska ; Goodrich, Justin (2015)

The Polycomb group (PcG) and trithorax group (trxG) genes play crucial roles in development by regulating expression of homeotic and other genes controlling cell fate. Both groups catalyse modifications of chromatin, particularly histone methylation, leading to epigenetic changes that affect gene activity. The trxG antagonizes the function of PcG genes by activating PcG target genes, and conseq...

Anti-adenoviral artificial microRNAs Expressed from AAV9 vectors inhibit human adenovirus infection in immunosuppressed Syrian hamsters

Schaar, Katrin ; Geisler, Anja ; Kraus, Milena ; Pinkert, Sandra ; Pryshliak, Markian ; Spencer, Jacqueline F. ; Tollefson, Ann E. ; Ying, Baoling ; Kurreck, Jens ; Wold, William S. ; Klopfleisch, Robert ; Toth, Karoly ; Fechner, Henry (2017-09)

Infections of immunocompromised patients with human adenoviruses (hAd) can develop into life-threatening conditions, whereas drugs with anti-adenoviral efficiency are not clinically approved and have limited efficacy. Small double-stranded RNAs that induce RNAi represent a new class of promising anti-adenoviral therapeutics. However, as yet, their efficiency to treat hAd5 infections has only be...

Hepatitis E virus superinfection and clinical progression in hepatitis B patients

Hoan, Nghiem Xuan ; Tong, Hoang Van ; Hecht, Nicole ; Sy, Bui Tien ; Marcinek, Patrick ; Meyer, Christian G. ; Song, Le Huu ; Toan, Nguyen Linh ; Kurreck, Jens ; Kremsner, Peter G. ; Bock, C-Thomas ; Velavan, Thirumalaisamy P. (2015)

Hepatitis E virus (HEV) infection may cause acute hepatitis and lead to hepatic failure in developing and developed countries. We studied HEV seroprevalences in patients with hepatitis B virus (HBV) infection to understand the consequences of HEV superinfection in a Vietnamese population. This cross-sectional study was conducted from 2012 to 2013 and included 1318 Vietnamese patients with HBV-r...

How a fungus shapes biotechnology: 100 years of Aspergillus niger research

Cairns, Timothy C. ; Nai, Corrado ; Meyer, Vera (2018-05-24)

In 1917, a food chemist named James Currie made a promising discovery: any strain of the filamentous mould Aspergillus niger would produce high concentrations of citric acid when grown in sugar medium. This tricarboxylic acid, which we now know is an intermediate of the Krebs cycle, had previously been extracted from citrus fruits for applications in food and beverage production. Two years afte...

Conditional expression of the small GTPase ArfA impacts secretion, morphology, growth, and actin ring position in Aspergillus niger

Fiedler, Markus R. M. ; Cairns, Timothy C. ; Koch, Oliver ; Kubisch, Christin ; Meyer, Vera (2018-05-08)

In filamentous fungi, growth and protein secretion occurs predominantly at the tip of long, thread like cells termed hyphae. This requires coordinated regulation of multiple processes, including vesicle trafficking, exocytosis, and endocytosis, which are facilitated by a complex cytoskeletal apparatus. In this study, functional analyses of the small GTPase ArfA from Aspergillus niger demonstrat...

Chip-based human liver-intestine and liver-skin co-culture

Maschmeyer, Ilka ; Hasenberg, Tobias ; Jaenicke, Annika ; Lindner, Marcus ; Lorenz, Alexandra Katharina ; Zech, Julie ; Garbe, Leif-Alexander ; Sonntag, Frank ; Hayden, Patrick ; Ayehunie, Seyoum ; Lauster, Roland ; Marx, Uwe ; Materne, Eva-Maria (2015)

Systemic repeated dose safety assessment and systemic efficacy evaluation of substances are currently carried out on laboratory animals and in humans due to the lack of predictive alternatives. Relevant international regulations, such as OECD and ICH guidelines, demand long-term testing and oral, dermal, inhalation, and systemic exposure routes for such evaluations. So-called “human-on-a-chip” ...

A single α helix drives extensive remodeling of the proteasome lid and completion of regulatory particle assembly

Tomko, Robert J. ; Taylor, David W. ; Chen, Zhuo A. ; Wang, Hong-Wei ; Rappsilber, Juri ; Hochstrasser, Mark (2015)

Most short-lived eukaryotic proteins are degraded by the proteasome. A proteolytic core particle (CP) capped by regulatory particles (RPs) constitutes the 26S proteasome complex. RP biogenesis culminates with the joining of two large subcomplexes, the lid and base. In yeast and mammals, the lid appears to assemble completely before attaching to the base, but how this hierarchical assembly is en...

The Aspergillus giganteus antifungal protein AFPNN5353activates the cell wall integrity pathway and perturbs calcium homeostasis

Binder, Ulrike ; Bencina, Mojca ; Eigentler, Andrea ; Meyer, Vera ; Marx, Florentine (2011-09-23)

Background The antifungal protein AFPNN5353 is a defensin-like protein of Aspergillus giganteus. It belongs to a group of secretory proteins with low molecular mass, cationic character and a high content of cysteine residues. The protein inhibits the germination and growth of filamentous ascomycetes, including important human and plant pathogens and the model organsims Aspergillus nidulans and...

Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii

Schmid, Jochen ; Müller-Hagen, Dirk ; Bekel, Thomas ; Funk, Laura ; Stahl, Ulf ; Sieber, Volker ; Meyer, Vera (2010-05-26)

Background The plant pathogenic basidiomycete Sclerotium rolfsii produces the industrially exploited exopolysaccharide scleroglucan, a polymer that consists of (1 → 3)-β-linked glucose with a (1 → 6)-β-glycosyl branch on every third unit. Although the physicochemical properties of scleroglucan are well understood, almost nothing is known about the genetics of scleroglucan biosynthesis. Similar...

The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans

dos Reis, Thaila Fernanda ; Nitsche, Benjamin M. ; de Lima, Pollyne Borborema Almeida ; de Assis, Leandro José ; Mellado, Laura ; Harris, Steven D. ; Meyer, Vera ; dos Santos, Renato A. Corrêa ; Riaño-Pachón, Diego M. ; Ries, Laure Nicolas Annick ; Goldman, Gustavo H. (2017-03-31)

One of the drawbacks during second-generation biofuel production from plant lignocellulosic biomass is the accumulation of glucose, the preferred carbon source of microorganisms, which causes the repression of hydrolytic enzyme secretion by industrially relevant filamentous fungi. Glucose sensing, subsequent transport and cellular signalling pathways have been barely elucidated in these organis...