FG Medizinische Biotechnologie

47 Items

Recent Submissions
3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone

Amler, Anna-Klara ; Thomas, Alexander ; Tüzüner, Selin ; Lam, Tobias ; Geiger, Michel-Andreas ; Kreuder, Anna-Elisabeth ; Palmer, Chris ; Nahles, Susanne ; Lauster, Roland ; Kloke, Lutz (2021-03-01)

Jawbone differs from other bones in many aspects, including its developmental origin and the occurrence of jawbone-specific diseases like MRONJ (medication-related osteonecrosis of the jaw). Although there is a strong need, adequate in vitro models of this unique environment are sparse to date. While previous approaches are reliant e.g. on scaffolds or spheroid culture, 3D bioprinting enables f...

Histone Deacetylases in the Inflamed Intestinal Epithelium—Promises of New Therapeutic Strategies

Gerbeth, Lorenz ; Glauben, Rainer (2021-03-26)

The intestinal epithelium is a complex, dynamic barrier that separates luminal contents from the immune compartment while mediating nutrient absorption and controlled passage of antigens to convey oral tolerance. A compromised epithelial barrier often leads to inflammation because immune cells in the lamina propria come into direct contact with luminal antigens. Defects in epithelial cell funct...

Inspired by the human placenta: a novel 3D bioprinted membrane system to create barrier models

Kreuder, Anna-Elisabeth ; Bolaños-Rosales, Aramis ; Palmer, Christopher ; Thomas, Alexander ; Geiger, Michel-Andreas ; Lam, Tobias ; Amler, Anna-Klara ; Markert, Udo R. ; Lauster, Roland ; Kloke, Lutz (2020-09-24)

Barrier organ models need a scaffold structure to create a two compartment culture. Technical filter membranes used most often as scaffolds may impact cell behaviour and present a barrier themselves, ultimately limiting transferability of test results. In this work we present an alternative for technical filter membrane systems: a 3D bioprinted biological membrane in 24 well format. The biologi...

Characterization of plasma cell subsets in the human bone marrow

Glenzer, Annika (2021)

Human bone marrow plasma cells (BMPC) are continuously producing antibodies and thereby, together with other mechanisms, protect the human body against foreign pathogens. While BMPC are not proliferating and have specific requirements for their survival niche, e.g. contact of adhesion molecules expressed on different cell types or secreted factors by surrounding cells. PC are the producers of a...

CD40 stimulation activates ‘post-activated’ B Cells which are hyporesponsive to B Cell receptor and toll-like receptor 9 stimulation in autoimmunity

Weißenberg, Sarah Yasmin (2021)

Autoimmunity arise when the immune system attacks self-antigens and destroys body’s own cells and tissues. Chronic autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and the primary Sjögren’s syndrome (pSS) decrease life quality of patients and can lead to life threatening events such as lupus nephritis [1]. Recognition of self-antigens by the B cell recep...

Effects of 5-aza-2´-deoxycytidine on primary human chondrocytes from osteoarthritic patients

Kadler, Shirin ; Vural, Özlem ; Rosowski, Jennifer ; Reiners-Schramm, Luzia ; Lauster, Roland ; Rosowski, Mark (2020-06-23)

Chondrocytes, comparable to many cells from the connective tissue, dedifferentiate and end up in a similar fibroblastoid cell type, marked by the loss of the specific expression pattern. Here, chondrocytes isolated from osteoarthritic (OA) patients were investigated. The OA chondrocytes used in this work were not affected by the loss of specific gene expression upon cell culture. The mRNA level...

Extracellular matrix- and pluripotent stem cell-based tissue engineering of the kidney

Fischer, Iris (2020)

Complex, three-dimensional (3D) organ models are novel biotechnological tools for research on regeneration and disease mechanisms as well as drug development. An organ model of the kidney is urgently needed to break through decades of stagnation in the development of new treatment methods for patients with chronic kidney disease and to reduce the high failure rate of drugs in clinical tests due...

Decoding the metabolic program of deregulated MYCN expression in neuroblastoma

Arlt, Birte (2020)

The oncogene MYCN is amplified in approximately 22% of all neuroblastomas and correlates with aggressive disease and unfavorable patient survival. Directly targeting MYCN remains a challenge due to its biochemical structure. We aimed for a deeper understanding of key metabolic pathways associated to amplified MYCN to discover druggable nodes that can be directly targeted for high-risk neuroblas...

IL-2 Therapy Diminishes Renal Inflammation and the Activity of Kidney-Infiltrating CD4+ T Cells in Murine Lupus Nephritis

Rose, Angelika ; von Spee-Mayer, Caroline ; Kloke, Lutz ; Wu, Kaiyin ; Kühl, Anja ; Enghard, Philipp ; Burmester, Gerd-Rüdiger ; Riemekasten, Gabriela ; Humrich, Jens (2019-10-11)

An acquired deficiency of interleukin-2 (IL-2) and related disturbances in regulatory T cell (Treg) homeostasis play an important role in the pathogenesis of systemic lupus erythematosus (SLE). Low-dose IL-2 therapy was shown to restore Treg homeostasis in patients with active SLE and its clinical efficacy is currently evaluated in clinical trials. Lupus nephritis (LN), a challenging organ mani...

Spatiotemporal RNA expression in the Caenorhabditis elegans germline

Diag, Asija (2020)

Spatiotemporally-restricted RNA expression is a crucial, conserved mechanism for maintaining tissue viability, morphology, and integrity. In particular, localization of RNA molecules to subcellular compartments is a prevalent mechanism for regulating the balance between cell proliferation and differentiation during development. Perturbations in the regulation of this balance are often associate...

In vitro validation of peptide-T3 conjugates as a treatment option for MCT8-deficiency via a “Trojan Horse”-like mechanism

Paisdzior, Sarah (2019)

Cells of an organism have to take up all substances needed for survival and function to maintain normal performance. Several hormones are crucial for a huge variety of different functions. Some of these hormones are transported over the plasma membranes by specific hormone transporters. Inactivating mutations in the genes of these transporters can lead to severe pathological phenotypes. To over...

The Trace Amine-Associated Receptor 1 Agonist 3-Iodothyronamine Induces Biased Signaling at the Serotonin 1b Receptor

Bräunig, Julia ; Dinter, Juliane ; Höfig, Carolin S. ; Paisdzior, Sarah ; Szczepek, Michal ; Scheerer, Patrick ; Rosowski, Mark ; Mittag, Jens ; Kleinau, Gunnar ; Biebermann, Heike (2018-03-12)

Trace amine-associated receptors (TAARs) belong to the class A G-protein-coupled receptors (GPCR) and are evolutionary related to aminergic receptors. TAARs have been identified to mediate effects of trace amines. TAAR1 signaling is mainly mediated via activation of the Gs/adenylyl cyclase pathway. In addition to classical trace amines, TAAR1 can also be activated by the thyroid hormone derivat...

Delayed local delivery of synthetic prostacyclin to modulate inflammatory processes in support of bone regeneration

Wendler, Sebastian (2019)

Delayed fracture healing or non-unions are relevant challenges in the clinical setting with a prevalence of more than 10 % of the regeneration outcomes. Autologous bone grafting or local application of growth factors such as bone morphogenic protein 2 (BMP-2) are considered to be current therapeutic gold standards. However, BMP-2 is expansive and over-dosage led to reports of negative side effe...

The calcineurin inhibitor cyclosporine A activates the renal Na-(K)-Cl cotransporters via local and systemic mechanisms

Blankenstein, Katharina Ilse (2019)

The two calcineurin inhibitors (CNIs) cyclosporine A (CsA) and tacrolimus are potent immunosuppressive drugs that have become indispensable in the post-transplantational therapy and in the treatment of autoimmune diseases. Immunosuppression via CNIs is achieved by inhibition of the calcineurin phosphatase and thus blocking the signaling pathway of NFAT (nuclear factor of activated T cells), the...

Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications

Schimek, Katharina ; Hsu, Hao-Hsiang ; Boehme, Moritz ; Kornet, Jacob Jan ; Marx, Uwe ; Lauster, Roland ; Pörtner, Ralf ; Lindner, Gerd (2018-06-07)

The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attracti...

Engineering Blood and Lymphatic Microvascular Networks in Fibrin Matrices

Knezevic, Lea ; Schaupper, Mira ; Mühleder, Severin ; Schimek, Katharina ; Hasenberg, Tobias ; Marx, Uwe ; Priglinger, Eleni ; Redl, Heinz ; Holnthoner, Wolfgang (2017-04-18)

Vascular network engineering is essential for nutrient delivery to tissue-engineered constructs and, consequently, their survival. In addition, the functionality of tissues also depends on tissue drainage and immune cell accessibility, which are the main functions of the lymphatic system. Engineering both the blood and lymphatic microvasculature would advance the survival and functionality of t...

3-Iodothyronamine Activates a Set of Membrane Proteins in Murine Hypothalamic Cell Lines

Bräunig, Julia ; Mergler, Stefan ; Jyrch, Sabine ; Hoefig, Carolin S. ; Rosowski, Mark ; Mittag, Jens ; Biebermann, Heike ; Khajavi, Noushafarin (2018-09-11)

3-Iodothyronamine (3-T1AM) is an endogenous thyroid hormone metabolite. The profound pharmacological effects of 3-T1AM on energy metabolism and thermal homeostasis have raised interest to elucidate its signaling properties in tissues that pertain to metabolic regulation and thermogenesis. Previous studies identified G protein-coupled receptors (GPCRs) and transient receptor potential channels (...

Bioprinting Perfusion-Enabled Liver Equivalents for Advanced Organ-on-a-Chip Applications

Grix, Tobias ; Ruppelt, Alicia ; Thomas, Alexander ; Amler, Anna-Klara ; Noichl, Benjamin P. ; Lauster, Roland ; Kloke, Lutz (2018-03-22)

Many tissue models have been developed to mimic liver-specific functions for metabolic and toxin conversion in in vitro assays. Most models represent a 2D environment rather than a complex 3D structure similar to native tissue. To overcome this issue, spheroid cultures have become the gold standard in tissue engineering. Unfortunately, spheroids are limited in size due to diffusion barriers in ...

Altered bone formation response to mechanical loading in a mouse model of the progeroid disorder gerodermia osteodysplastica

Thelen, Michael (2019)

Bone tissue has to constantly adapt to changing mechanical requirements. The mechanisms behind mechanosensation, mechanotransduction, signal transmission and the resulting response of bone tissue are, until this day not fully understood. A failure of these processes leads to inappropriate bone architecture, bone mass and reduced mechanical stability as seen in age-related osteoporosis or rare d...

Protokoll zur Viromanalyse bei Infektionen unklarer Genese

Klenner, Jeanette (2019)

Next Generation Sequencing (NGS) eröffnete eine Vielzahl neuer Forschungsfelder in der Medizin und der Biologie. Der Einsatz von NGS-Technologien, wie z.B. der Metagenomik, reicht heute von der Grundlagenforschung bis hin zur angewandten Forschung. Allgemein wird davon ausgegangen, dass die Technologie des NGS schon in naher Zukunft in der klinischen Routinediagnostik eingesetzt werden wird. Me...