FG Medizinische Biotechnologie

17 Items

Recent Submissions
Role of autoantigen-specific T cell subsets in disease pathogenesis and as therapeutic tool in systemic lupus erythematosus

Abdirama, Dimas (2017)

Systemic lupus erythematosus (SLE) is a chronic and systemic autoimmune disorder characterized by loss of immune tolerance towards nuclear antigens such as SmD1, RNP70, Histone, Ro, and La. Besides the production of autoantibodies that directed to nuclear antigens, CD4+ T cells with T cell receptor specific to nuclear antigens may also trigger autoimmune responses leading to severe organ inflam...

Automated universal chip platform for fluorescence based cellular assays

Schmieder, F. ; Schmieder, S. ; Eger, R. ; Friedrich, S. ; Werner, A. ; Danz, N. ; Marx, Uwe ; Sonntag, Frank (2012)

The advantage of cell based assays used as biosensors is the direct access to hardly obtainable parameters like toxicity, mutagenicity and pharmacological effectiveness. Within the last few years we established a micro fluidic platform including a peristaltic micro pump as well as several valves, manifolds and micro channels [1]. For optical online monitoring the micro fluidic system is bonded ...

Hollow fibres integrated in a microfluidic cell culture system

Winkelmann, Claudia ; Luo, Y. ; Lode, A. ; Gelinsky, M. ; Marx, Uwe ; Sonntag, Frank (2012)

For in vitro drug screening a modified perfusion micro-bioreactor system with integrated hollow fibres will be demonstrated. This biocompatible system consists of an integrated closed flow circuit that includes reservoirs and pneumatic micro pumps. Additional optical online-monitoring devices allow the observation during the cell cultivation. The embedded hollow fibre system which acts as cell ...

Automated Micro-PIV measurement in Lab-on-a-Chip systems

Busek, Mathias ; Polk, Christoph ; Albrecht, Thomas ; Marx, Uwe ; König, J. ; Sonntag, Frank (2012)

Flow rate and wall shear stress are important parameters for perfused cell culture systems and should be monitored. An easy and non-invasive method is the particle image velocimetry (PIV). In this work PIV was used to characterize a cell culture system with included peristaltic pump. The time-dependent flow profile was measured on several points of the chip for different pumping speeds to figur...

Etablierung und Charakterisierung eines dreidimensionalen Zellkulturmodells für Kuhpockeninfektionen

Koban, Robert (2017)

Zellkultivierungen stellen ein elementares Werkzeug für die Erforschung vielfältiger biologischer Phänomene dar. In der Virologie werden Infektionen in vitro fast ausschließlich in Monolayer-Kulturen charakterisiert und erforscht, in denen Zellen flach ausgebreitet auf starren Oberflächen kultiviert werden und im Vergleich zum in vivo Status der gleichen Zellen deutliche Unterschiede bezüglich ...

A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents

Maschmeyer, Ilka ; Lorenz, Alexandra K. ; Schimek, Katharina ; Hasenberg, Tobias ; Ramme, Anja P. ; Hübner, Juliane ; Lindner, Marcus ; Drewell, Christopher ; Bauer, Sophie ; Thomas, Alexander ; Sambo, Naomia Sisoli ; Sonntag, Frank ; Lauster, Roland ; Marx, Uwe (2015)

Systemic absorption and metabolism of drugs in the small intestine, metabolism by the liver as well as excretion by the kidney are key determinants of efficacy and safety for therapeutic candidates. However, these systemic responses of applied substances lack in most in vitro assays. In this study, a microphysiological system maintaining the functionality of four organs over 28 days in co-cultu...

Ex vivo Charakterisierung von humanen Autoantigen-spezifischen CD4+ T-Zellen mit Fokus auf Multiple Sklerose

Gamradt, Stefanie (2017)

Trotz intensiver Forschung ist unklar, ob Myelin-reaktive CD4+ T-Zellen in der Pathogenese der Multiplen Sklerose (MS) eine relevante Rolle spielen. Ein Grund dafür ist das Fehlen einer Methode für ihre direkte Analyse. In dieser Arbeit ist es mit Hilfe der magnetischen Anreicherung von spezifisch-aktivierten CD154+ konventionellen und CD137+ regulato-rischen T-Zellen in Kombination mit multipa...

Die Induktion von Interleukin-10 in humanen T-Helfer-Zellen und seine transkriptionelle Regulation

Ahlers, Jonas (2017)

Die Produktion des anti-inflammatorischen Zytokins IL-10 von Th-Zellen ist ein wesentlicher Mechanismus zur Regulation von Immunantworten. Hierbei können professionelle regulatorische T-Zellen suppressive Funktionen übernehmen oder IL-10-produzierende Th-Zellen sich selber regulieren. In dieser Arbeit konnte gezeigt werden, dass die Stimulation mit den Zytokinen IFN-α, IL-6 und IL-21 über den...

Partielle Charakterisierung der neuartigen humanen Polyomaviren HPyV9 und HPyV12

Korup-Schulz, Sarah-Verena (2017)

Im letzten Jahrzehnt wurden elf neue humane Polyomaviren (PyV) und zahlreiche tierische PyV identifiziert. Die Charakteristika dieser PyV wie Gewebetropismus, zelluläre Eintritts- und Infektionsmechanismen sind bislang ungeklärt. Humanes PyV 9 (HPyV9), im Serum eines Nierentransplantat-Empfängers entdeckt, und Humanes PyV 12 (HPyV12), in Proben von kolorektalen Lebermetastasen identifiziert, si...

Synthetic biomaterial microenvironments to modulate paracrine effects of mesenchymal stromal cells for skeletal muscle regeneration

Qazi, Taimoor Hasan (2017)

While skeletal muscles can recover from minor injuries, severe trauma can often induce irreversible structural damage such as extensive scar tissue formation and damaged muscle fibers. This leads to functional deficits and loss in the quality of life of patients. Effective treatment of severe muscle injuries is currently an unmet clinical need. In such cases, a therapeutic intervention such as ...

Tissue engineered cartilage: how hard can it be?

Kadler, Shirin (2017)

Für die Weiterentwicklung regenerativer Therapien sind humane in vitro Modelle aus Primärzellen entscheidend. Die Isolation und Expansion primärer Zellen zieht den Verlust der Gewebsspezifität nach sich. Diese Dedifferenzierung ist in allen Zellen des Mesenchyms zu finden und ist charakterisiert durch den Verlust der Expression von Gewebsmarkern und einer spindelförmigen Zellform durch Plastika...

Investigation of experimental cell lines and non-invasive online sensor technologies in a 3D bioreactor system for extracorporeal liver support therapy

Richter, Marco (2016)

Liver transplantation is currently the only successful treatment to cure acute or acute-on-chronic liver failure. However, the number of patients waiting for a donor organ is constantly rising due to scarcity of organ donations. To overcome this bottleneck different bio-artificial liver technologies are under investigation, which may take over functions of the diseased liver until a suitable do...

The role of mast cells in the immune response against bacterial infections

Zimmermann, Carolin (2016)

Skin wound infections are a significant and increasing health problem, and antibiotic resistance is increasing. Mast cells have been shown to contribute to host defence responses in bacterial infections. Their role in skin wound infection is presently unknown. We subjected several mast cell-deficient mouse strains to Pseudomonas aeruginosa skin wound infection and found significantly delaye...

Fluorescent optical fiber sensors for cell viability monitoring

Sergachev, Ilia ; Rusanov, Alexander ; Trushkin, Evgeny ; Sakharov, Dmitry ; Marx, Uwe ; Tonevitsky, Alexander (2013)

A new simple method for non-invasive cell culture viability monitoring based on vital fluorescent stains is introduced, and its efficiency for long-term experiments on cells is demonstrated. In contrast to common methods for cell viability control, which are usually either destructive (like flow-type counters or dead cells coloring and counting), or hardly quantitative like fluorescent microsco...

Chip-based liver equivalents for toxicity testing - organotypicalness versus cost-efficient high throughput

Materne, Eva-Maria ; Tonevitsky, Alexander G. ; Marx, Uwe (2013)

Drug-induced liver toxicity dominates the reasons for pharmaceutical product ban, withdrawal or non-approval since the thalidomide disaster in the late-1950s. Hopes to finally solve the liver toxicity test dilemma have recently risen to a historic level based on the latest progress in human microfluidic tissue culture devices. Chip-based human liver equivalents are envisaged to identify liver t...

Integrating biological vasculature into a multi-organ-chip microsystem

Schimek, Katharina ; Busek, Mathias ; Brincker, Sven ; Groth, Benjamin ; Hoffmann, Silke ; Lauster, Roland ; Lindner, Gerd ; Lorenz, Alexandra ; Menzel, Ulrike ; Sonntag, Frank ; Walles, Heike ; Marx, Uwe ; Horland, Reyk (2013)

A chip-based system mimicking the transport function of the human cardiovascular system has been established at minute but standardized microsystem scale. A peristaltic on-chip micropump generates pulsatile shear stress in a widely adjustable physiological range within a microchannel circuit entirely covered on all fluid contact surfaces with human dermal microvascular endothelial cells. This m...

A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture

Wagner, Ilka ; Materne, Eva-Maria ; Brincker, Sven ; Süßbier, Ute ; Frädrich, Caroline ; Busek, Mathias ; Sonntag, Frank ; Sakharov, Dmitry A. ; Trushkin, Evgeny V. ; Tonevitsky, Alexander G. ; Lauster, Roland ; Marx, Uwe (2013)

Current in vitro and animal tests for drug development are failing to emulate the systemic organ complexity of the human body and, therefore, to accurately predict drug toxicity. In this study, we present a multi-organ-chip capable of maintaining 3D tissues derived from cell lines, primary cells and biopsies of various human organs. We designed a multi-organ-chip with co-cultures of human artif...