FG Medizinische Biotechnologie

34 Items

Recent Submissions
The calcineurin inhibitor cyclosporine A activates the renal Na-(K)-Cl cotransporters via local and systemic mechanisms

Blankenstein, Katharina Ilse (2019)

The two calcineurin inhibitors (CNIs) cyclosporine A (CsA) and tacrolimus are potent immunosuppressive drugs that have become indispensable in the post-transplantational therapy and in the treatment of autoimmune diseases. Immunosuppression via CNIs is achieved by inhibition of the calcineurin phosphatase and thus blocking the signaling pathway of NFAT (nuclear factor of activated T cells), the...

Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications

Schimek, Katharina ; Hsu, Hao-Hsiang ; Boehme, Moritz ; Kornet, Jacob Jan ; Marx, Uwe ; Lauster, Roland ; Pörtner, Ralf ; Lindner, Gerd (2018-06-07)

The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attracti...

Engineering Blood and Lymphatic Microvascular Networks in Fibrin Matrices

Knezevic, Lea ; Schaupper, Mira ; Mühleder, Severin ; Schimek, Katharina ; Hasenberg, Tobias ; Marx, Uwe ; Priglinger, Eleni ; Redl, Heinz ; Holnthoner, Wolfgang (2017-04-18)

Vascular network engineering is essential for nutrient delivery to tissue-engineered constructs and, consequently, their survival. In addition, the functionality of tissues also depends on tissue drainage and immune cell accessibility, which are the main functions of the lymphatic system. Engineering both the blood and lymphatic microvasculature would advance the survival and functionality of t...

3-Iodothyronamine Activates a Set of Membrane Proteins in Murine Hypothalamic Cell Lines

Bräunig, Julia ; Mergler, Stefan ; Jyrch, Sabine ; Hoefig, Carolin S. ; Rosowski, Mark ; Mittag, Jens ; Biebermann, Heike ; Khajavi, Noushafarin (2018-09-11)

3-Iodothyronamine (3-T1AM) is an endogenous thyroid hormone metabolite. The profound pharmacological effects of 3-T1AM on energy metabolism and thermal homeostasis have raised interest to elucidate its signaling properties in tissues that pertain to metabolic regulation and thermogenesis. Previous studies identified G protein-coupled receptors (GPCRs) and transient receptor potential channels (...

Bioprinting Perfusion-Enabled Liver Equivalents for Advanced Organ-on-a-Chip Applications

Grix, Tobias ; Ruppelt, Alicia ; Thomas, Alexander ; Amler, Anna-Klara ; Noichl, Benjamin P. ; Lauster, Roland ; Kloke, Lutz (2018-03-22)

Many tissue models have been developed to mimic liver-specific functions for metabolic and toxin conversion in in vitro assays. Most models represent a 2D environment rather than a complex 3D structure similar to native tissue. To overcome this issue, spheroid cultures have become the gold standard in tissue engineering. Unfortunately, spheroids are limited in size due to diffusion barriers in ...

Altered bone formation response to mechanical loading in a mouse model of the progeroid disorder gerodermia osteodysplastica

Thelen, Michael (2019)

Bone tissue has to constantly adapt to changing mechanical requirements. The mechanisms behind mechanosensation, mechanotransduction, signal transmission and the resulting response of bone tissue are, until this day not fully understood. A failure of these processes leads to inappropriate bone architecture, bone mass and reduced mechanical stability as seen in age-related osteoporosis or rare d...

Protokoll zur Viromanalyse bei Infektionen unklarer Genese

Klenner, Jeanette (2019)

Next Generation Sequencing (NGS) eröffnete eine Vielzahl neuer Forschungsfelder in der Medizin und der Biologie. Der Einsatz von NGS-Technologien, wie z.B. der Metagenomik, reicht heute von der Grundlagenforschung bis hin zur angewandten Forschung. Allgemein wird davon ausgegangen, dass die Technologie des NGS schon in naher Zukunft in der klinischen Routinediagnostik eingesetzt werden wird. Me...

Emulating the early phases of human tooth development in vitro

Rosowski, Jennifer ; Bräunig, Julia ; Amler, Anna-Klara ; Strietzel, Frank P. ; Lauster, Roland ; Rosowski, Mark (2019-05-07)

Functional in vitro models emulating the physiological processes of human organ formation are invaluable for future research and the development of regenerative therapies. Here, a developmentally inspired approach is pursued to reproduce fundamental steps of human tooth organogenesis in vitro using human dental pulp cells. Similar to the in vivo situation of tooth initiating mesenchymal condens...

3-Iodothyronamine and its role in aminergic G-protein coupled receptor signaling and neuromodulation

Bräunig, Julia (2019)

3-Iodothyronamine (T1AM) is an endogenous thyroid hormone metabolite. Its profound pharmacological effects on energy metabolism and thermal homeostasis in rodents have raised interest to elucidate its signaling properties in tissues that pertain to metabolic regulation and thermogenesis. Especially its anapyrexic effect could be utilized in emergency medicine to treat disease conditions like st...

Analyse der Hyperinflammation im Verlauf der murinen Pneumokokken-Pneumonie unter besonderer Berücksichtigung des Neutrophile-rekrutierenden Chemokins CXCL5

Berger, Sarah (2019)

Die ambulant erworbene Pneumonie ist mit ihrer hohen Inzidenz und Mortalität eine der bedeutendsten Infektionskrankheiten weltweit. Streptococcus pneumoniae (S. pn.) ist der häufigste Erreger der ambulant erworbenen Pneumonie. Eine wichtige Ursache für die hohe Sterblichkeit trotz wirksamer antibiotischer Therapie stellt die umgreifende Schädigung eines inadäquat aktivierten Immunsystems währen...

Model for tooth development in vitro

Rosowski, Jennifer (2019)

The ultimate goal of Tissue Engineering is the regeneration of whole organs or functional organ units. Recent approaches rely on the usage of adult tissue-specific cells in three-dimensional culture. It is now accepted that "Developmental Engineering", the recapitulation of critical initial steps of organogenesis in vitro, is more expedient. Identification of key players and basic mechanisms be...

In vivo-Analyse der B-Zell-Rezeptor-Signaltransduktion im Keimzentrum

Ulbricht, Carolin (2018)

Unser Immunsystem stellt uns eine Vielzahl von Effektormechanismen zur Verfügung, die für den lebenslangen Schutz vor Krankheitserregern sorgen. Ein Aspekt dieser Mechanismen ist die Produktion von Immunglobulin-Antikörpern durch langlebige Plasmazellen, die aus B-Zellen in Keimzentren der sekundären lymphatischen Organe stammen. Innerhalb der Keimzentren bilden B-Zellen, T-Zellen und follikulä...

Monitoring of the single-cell morphology for the evaluation of microbial eukaryotic bioprocesses

Marbà Ardébol, Anna Maria (2018)

Cell morphology is not only influenced by the cell cycle, the aging or individual properties, but also by environmental impacts such as those occurring on a large-scale. Cell morphology can be a suitable parameter for in situ measurements as it changes dynamically and is often related to cell physiology. In order to be able to identify relationships between cell physiology and morphology, stati...

P45 IL-2 therapy reduces renal inflammation and cellular activity of intrarenal CD4+ conventional T cells in lupus prone mice with active lupus nephritis

Rose, A. ; von Spee-Mayer, C. ; Kloke, Lutz ; Wu, K. ; Enghard, P. ; Kühl, A. ; Humrich, J.Y. ; Riemekasten, G. (2016-11)

null

Chip-based human liver-intestine and liver-skin co-culture

Maschmeyer, Ilka ; Hasenberg, Tobias ; Jaenicke, Annika ; Lindner, Marcus ; Lorenz, Alexandra Katharina ; Zech, Julie ; Garbe, Leif-Alexander ; Sonntag, Frank ; Hayden, Patrick ; Ayehunie, Seyoum ; Lauster, Roland ; Marx, Uwe ; Materne, Eva-Maria (2015)

Systemic repeated dose safety assessment and systemic efficacy evaluation of substances are currently carried out on laboratory animals and in humans due to the lack of predictive alternatives. Relevant international regulations, such as OECD and ICH guidelines, demand long-term testing and oral, dermal, inhalation, and systemic exposure routes for such evaluations. So-called “human-on-a-chip” ...

Emulating the human vasculature in a Multi-Organ-Chip platform

Hasenberg, Tobias (2018)

The Multi-Organ-Chip platform is a microphysiological system developed to evaluate toxicity and efficacy of drugs, and adverse effects of cosmetics, chemicals and alike in a sub-systemic mode. At the scale of a microscope glass slide, it comprises several compartments for the co-cultivation of human 3D tissue constructs. The organoids are physically separated, yet, interconnected through perfus...

Optimizing human Treg stability and target specificity for therapeutic applications

Nowak, Anna (2018)

Regulatory T cells (Tregs) are essential mediators of tolerance and crucial for the containment of inflammatory immune reactions. Their immunosuppressive potential is currently investigated for the treatment of inflammatory diseases. However, conversion between the Treg and conventional T cell (Tcon) compartment has been described, comprising the induction or loss of the regulatory phenotype in...

Role of autoantigen-specific T cell subsets in disease pathogenesis and as therapeutic tool in systemic lupus erythematosus

Abdirama, Dimas (2017)

Systemic lupus erythematosus (SLE) is a chronic and systemic autoimmune disorder characterized by loss of immune tolerance towards nuclear antigens such as SmD1, RNP70, Histone, Ro, and La. Besides the production of autoantibodies that directed to nuclear antigens, CD4+ T cells with T cell receptor specific to nuclear antigens may also trigger autoimmune responses leading to severe organ inflam...

Automated universal chip platform for fluorescence based cellular assays

Schmieder, F. ; Schmieder, S. ; Eger, R. ; Friedrich, S. ; Werner, A. ; Danz, N. ; Marx, Uwe ; Sonntag, Frank (2012)

The advantage of cell based assays used as biosensors is the direct access to hardly obtainable parameters like toxicity, mutagenicity and pharmacological effectiveness. Within the last few years we established a micro fluidic platform including a peristaltic micro pump as well as several valves, manifolds and micro channels [1]. For optical online monitoring the micro fluidic system is bonded ...

Hollow fibres integrated in a microfluidic cell culture system

Winkelmann, Claudia ; Luo, Y. ; Lode, A. ; Gelinsky, M. ; Marx, Uwe ; Sonntag, Frank (2012)

For in vitro drug screening a modified perfusion micro-bioreactor system with integrated hollow fibres will be demonstrated. This biocompatible system consists of an integrated closed flow circuit that includes reservoirs and pneumatic micro pumps. Additional optical online-monitoring devices allow the observation during the cell cultivation. The embedded hollow fibre system which acts as cell ...