FG Bioanalytik

7 Items

Recent Submissions
Functional inhibition of acid sphingomyelinase by Fluphenazine triggers hypoxia-specific tumor cell death

Klutzny, Saskia ; Lesche, Ralf ; Keck, Matthias ; Kaulfuss, Stefan ; Schlicker, Andreas ; Christian, Sven ; Sperl, Carolyn ; Neuhaus, Roland ; Mowat, Jeffrey ; Steckel, Michael ; Riefke, Björn ; Prechtl, Stefan ; Parczyk, Karsten ; Steigemann, Patrick (2017)

Owing to lagging or insufficient neo-angiogenesis, hypoxia is a feature of most solid tumors. Hypoxic tumor regions contribute to resistance against antiproliferative chemotherapeutics, radiotherapy and immunotherapy. Targeting cells in hypoxic tumor areas is therefore an important strategy for cancer treatment. Most approaches for targeting hypoxic cells focus on the inhibition of hypoxia adap...

Elucidation of orthopoxvirus adaptation mechanisms by proteomics and genomics

Grossegesse, Marica (2018)

Adaptive changes of viruses enable the virus-host coevolution and infection of new host species. While viruses with an RNA genome adapt primarily by genomic variabilities, adaptation mechanisms of viruses with a DNA genome have remained largely elusive. Since genomes of DNA viruses are more stable compared to genomes of RNA viruses, it is assumed that mechanisms other than genomic changes under...

Proteomics of a fuzzy organelle: interphase chromatin

Kustatscher, Georg ; Hégarat, Nadia ; Wills, Karen L. H. ; Furlan, Cristina ; Bukowski-Wills, Jimi-Carlo ; Hochegger, Helfrid ; Rappsilber, Juri (2014)

Chromatin proteins mediate replication, regulate expression, and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle, it defies classical organellar proteomics and canno...

Blind testing of cross-linking/mass spectrometry hybrid methods in CASP11

Schneider, Michael ; Belsom, Adam ; Rappsilber, Juri ; Brock, Oliver (2016)

Hybrid approaches combine computational methods with experimental data. The information contained in the experimental data can be leveraged to probe the structure of proteins otherwise elusive to computational methods. Compared with computational methods, the structures produced by hybrid methods exhibit some degree of experimental validation. In spite of these advantages, most hybrid methods h...

Multiclassifier combinatorial proteomics of organelle shadows at the example of mitochondria in chromatin data

Kustatscher, Georg ; Grabowski, Piotr ; Rappsilber, Juri (2016)

Subcellular localization is an important aspect of protein function, but the protein composition of many intracellular compartments is poorly characterized. For example, many nuclear bodies are challenging to isolate biochemically and thus remain inaccessible to proteomics. Here, we explore covariation in proteomics data as an alternative route to subcellular proteomes. Rather than targeting a ...

Transposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution

Davis, Matthew P. ; Carrieri, Claudia ; Saini, Harpreet K. ; Dongen, Stijn van ; Leonardi, Tommaso ; Bussotti, Giovanni ; Monahan, Jack M. ; Auchynnikava, Tania ; Bitetti, Angelo ; Rappsilber, Juri ; Allshire, Robin C. ; Shkumatava, Alena ; O'Carroll, Dónal ; Enright, Anton J. (2017)

Spermatogenesis is associated with major and unique changes to chromosomes and chromatin. Here, we sought to understand the impact of these changes on spermatogenic transcriptomes. We show that long terminal repeats (LTRs) of specific mouse endogenous retroviruses (ERVs) drive the expression of many long non-coding transcripts (lncRNA). This process occurs post-mitotically predominantly in sper...

Quality assurance in immunoassay performance – carbamazepine immunoassay format evaluation and application on surface and waste water

Grandke, Julia ; Oberleitner, Lidia ; Resch-Genger, Ute ; Garbe, Leif-Alexander ; Schneider, Rudolf J. (2013)

Carbamazepine (CBZ) is one of the most frequently detected pharmaceuticals in water samples. For the determination of this anthropogenic marker, various immunoassay formats were tested and evaluated in order to identify the most suitable one. For these direct competitive assays, the analyte was labelled with the enzyme horseradish peroxidase (HRP) or alkaline phosphatase (AP), and seven substra...