Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-5256
Main Title: Cell phenotypic changes of mouse connective tissue fibroblasts (L-929) to poly(ethylene glycol)-based gels
Author(s): Strehmel, Christine
Zhang, Zhenfang
Strehmel, Nadine
Lensen, Marga C.
Type: Article
Language Code: en
Abstract: Cellular responses to various gels fabricated by photoinitiated crosslinking using acrylated linear and multi-arm poly(ethylene glycol) (PEG)-based and poly(propylene glycol)-b-poly(ethylene glycol) precursors were investigated. While no protein adsorption and cell adhesion were observed on the hydrophilic PEG-based gels, protein adsorption and cell adhesion did occur on the more hydrophobic gel generated from the block copolymer precursor. Murine fibroblast viability on the poly(ethylene glycol)-based gels was studied in the course of 72 h and the results indicated no cytotoxicity. In a systematic study, extra- and intracellular metabolites of the murine fibroblasts cultured on these PEG-based gels were examined by GC-MS. Distinct intra- and extracellular changes in primary metabolism, namely amino acid metabolism, glycolysis and fatty acid metabolism, were observed. Cells cultured on the polymeric gels induced more intense intracellular changes in the metabolite profile by means of higher metabolite intensities with time in comparison to cells cultured on the reference substrate (tissue culture polystyrene). In contrast, extracellular changes of metabolite intensities were comparable.
URI: http://depositonce.tu-berlin.de/handle/11303/5636
http://dx.doi.org/10.14279/depositonce-5256
Issue Date: 2013
Date Available: 24-Jun-2016
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Sponsor/Funder: DFG, EXC 314, Unifying Concepts in Catalysis
Usage rights: Terms of German Copyright Law
Journal Title: Biomaterials Science
Publisher: Royal Society of Chemistry
Publisher Place: Cambridge
Volume: 1
Issue: 8
Publisher DOI: 10.1039/c3bm60055f
Page Start: 850
Page End: 859
EISSN: 2047-4830
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:Technische Universität Berlin » Fakultäten & Zentralinstitute » Fakultät 2 Mathematik und Naturwissenschaften » Institut für Chemie » Fachgebiet Nanostrukturierte Biomaterialien » Publications

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