Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-5265
Main Title: Integrating biological vasculature into a multi-organ-chip microsystem
Author(s): Schimek, Katharina
Busek, Mathias
Brincker, Sven
Groth, Benjamin
Hoffmann, Silke
Lauster, Roland
Lindner, Gerd
Lorenz, Alexandra
Menzel, Ulrike
Sonntag, Frank
Walles, Heike
Marx, Uwe
Horland, Reyk
Type: Article
Language Code: en
Abstract: A chip-based system mimicking the transport function of the human cardiovascular system has been established at minute but standardized microsystem scale. A peristaltic on-chip micropump generates pulsatile shear stress in a widely adjustable physiological range within a microchannel circuit entirely covered on all fluid contact surfaces with human dermal microvascular endothelial cells. This microvascular transport system can be reproducibly established within four days, independently of the individual endothelial cell donor background. It interconnects two standard tissue culture compartments, each of 5 mm diameter, through microfluidic channels of 500 μm width. Further vessel branching and vessel diameter reduction down to a microvessel scale of approximately 40 μm width was realised by a two-photon laser ablation technique applied to inserts, designed for the convenient establishment of individual organ equivalents in the tissue culture compartments at a later time. The chip layout ensures physiological fluid-to-tissue ratios. Moreover, an in-depth microscopic analysis revealed the fine-tuned adjustment of endothelial cell behaviour to local shear stresses along the microvasculature of the system. Time-lapse and 3D imaging two-photon microscopy were used to visualise details of spatiotemporal adherence of the endothelial cells to the channel system and to each other. The first indicative long-term experiments revealed stable performance over two and four weeks. The potential application of this system for the future establishment of human-on-a-chip systems and basic human endothelial cell research is discussed.
URI: http://depositonce.tu-berlin.de/handle/11303/5645
http://dx.doi.org/10.14279/depositonce-5265
Issue Date: 2013
Date Available: 24-Jun-2016
DDC Class: 004 Datenverarbeitung; Informatik
570 Biowissenschaften; Biologie
540 Chemie und zugeordnete Wissenschaften
Sponsor/Funder: BMBF, 0315569, GO-Bio 3: Multi-Organ-Bioreaktoren für die prädiktive Substanztestung im Chipformat
Usage rights: Terms of German Copyright Law
Journal Title: Lab on a chip : miniaturisation for chemistry and biology
Publisher: Royal Society of Chemistry
Publisher Place: Cambridge
Volume: 13
Issue: 18
Publisher DOI: 10.1039/c3lc50217a
Page Start: 3588
Page End: 3598
EISSN: 1473-0197
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:Technische Universität Berlin » Fakultäten & Zentralinstitute » Fakultät 3 Prozesswissenschaften » Institut für Biotechnologie » Fachgebiet Medizinische Biotechnologie » Publications

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