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Main Title: Quality assurance in immunoassay performance – carbamazepine immunoassay format evaluation and application on surface and waste water
Author(s): Grandke, Julia
Oberleitner, Lidia
Resch-Genger, Ute
Garbe, Leif-Alexander
Schneider, Rudolf J.
Type: Article
Language Code: en
Abstract: Carbamazepine (CBZ) is one of the most frequently detected pharmaceuticals in water samples. For the determination of this anthropogenic marker, various immunoassay formats were tested and evaluated in order to identify the most suitable one. For these direct competitive assays, the analyte was labelled with the enzyme horseradish peroxidase (HRP) or alkaline phosphatase (AP), and seven substrates with specific detection properties were used. The quality criteria for the standard curves were fulfilled by all HRP assays and the chemiluminescence AP format. Furthermore, intra- and inter-plate coefficients of variation as a measure of the achievable precision were determined for the samples. The application of the AP assays to surface water was unfeasible due to CBZ concentrations below the quantifiable concentration range. Surface as well as waste water samples could be analyzed with the HRP assays. Here, the HRP assay employing the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine yielded the best results.
Issue Date: 2013
Date Available: 27-Jun-2016
DDC Class: 540 Chemie und zugeordnete Wissenschaften
660 Chemische Verfahrenstechnik
Journal Title: Analytical methods
Publisher: Royal Society of Chemistry
Publisher Place: Cambridge
Volume: 5
Issue: 15
Publisher DOI: 10.1039/c3ay40702k
Page Start: 3754
Page End: 3760
EISSN: 1759-9660
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:FG Bioanalytik » Publications

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