FG Angewandte und Molekulare Mikrobiologie

49 Items

Recent Submissions
Ten simple rules for hosting artists in a scientific lab

Rillig, Matthias C. ; Bonneval, Karine ; Lutz, Christian de ; Lehmann, Johannes ; Mansour, India ; Rapp, Regine ; Spačal, Saša ; Meyer, Vera (2021-02-25)

Hosting an artist in a scientific lab is likely a new experience for many scientists in the natural and engineering sciences, and perhaps also for many artists, yet it can be a very beneficial experience for both parties [1]. “Art and science are in a tension that is most fruitful when these disciplines observe and penetrate each other and experience how much of the other they themselves still ...

Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger

Sui, Yu-fei ; Schütze, Tabea ; Ouyang, Li-ming ; Lu, Hongzhong ; Liu, Peng ; Xiao, Xianzun ; Qi, Jie ; Zhuang, Ying-Ping ; Meyer, Vera (2020-10-23)

Background: Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis in the filamentous fungal cell factory Aspergillus niger indicated that low availability of NADPH might be a limiting factor for GlaA overproduction. Res...

Secondary metabolite arsenal of an opportunistic pathogenic fungus

Bignell, Elaine ; Cairns, Timothy C. ; Throckmorton, Kurt ; Nierman, William C. ; Keller, Nancy P. (2016-12-05)

Aspergillus fumigatus is a versatile fungus able to successfully exploit diverse environments from mammalian lungs to agricultural waste products. Among its many fitness attributes are dozens of genetic loci containing biosynthetic gene clusters (BGCs) producing bioactive small molecules (often referred to as secondary metabolites or natural products) that provide growth advantages to the fungu...

Identification of SclB, a Zn(II)2Cys6 transcription factor involved in sclerotium formation in Aspergillus niger

Jørgensen, Thomas R. ; Burggraaf, Anne-Marie ; Arentshorst, Mark ; Schutze, Tabea ; Lamers, Gerda ; Niu, Jing ; Kwon, Min Jin ; Park, Joohae ; Frisvad, Jens C. ; Nielsen, Kristian F. ; Meyer, Vera ; Hondel, Cees A.M.J.J.van den ; Dyer, Paul S. ; Ram, Arthur F.J. (2020-04-03)

Certain Aspergillus species such as Aspergillus flavus and A. parasiticus are well known for the formation of sclerotia. These developmental structures are thought to act as survival structures during adverse environmental conditions but are also a prerequisite for sexual reproduction. We previously described an A. niger mutant (scl-2) which formed sclerotium-like structures, suggesting a possi...

A silver bullet in a golden age of functional genomics: the impact of Agrobacterium-mediated transformation of fungi

Idnurm, Alexander ; Bailey, Andy M. ; Cairns, Timothy C. ; Elliott, Candace E. ; Foster, Gary D. ; Ianiri, Giuseppe ; Jeon, Junhyun (2017-09-26)

The implementation of Agrobacterium tumefaciens as a transformation tool revolutionized approaches to discover and understand gene functions in a large number of fungal species. A. tumefaciens mediated transformation (AtMT) is one of the most transformative technologies for research on fungi developed in the last 20 years, a development arguably only surpassed by the impact of genomics. AtMT ha...

Systems approaches to predict the functions of glycoside hydrolases during the life cycle of Aspergillus niger using developmental mutants ∆brlA and ∆flbA

Munster, Jolanda M. van ; Nitsche, Benjamin M. ; Akeroyd, Michiel ; Dijkhuizen, Lubbert ; Maarel, Marc J. E. C. van der ; Ram, Arthur F. J. (2015-01-28)

Background The filamentous fungus Aspergillus niger encounters carbon starvation in nature as well as during industrial fermentations. In response, regulatory networks initiate and control autolysis and sporulation. Carbohydrate-active enzymes play an important role in these processes, for example by modifying cell walls during spore cell wall biogenesis or in cell wall degradation connected t...

A transcriptome meta-analysis proposes novel biological roles for the antifungal protein AnAFP in Aspergillus niger

Paege, Norman ; Jung, Sascha ; Schäpe, Paul ; Müller-Hagen, Dirk ; Ouedraogo, Jean-Paul ; Heiderich, Caroline ; Jedamzick, Johanna ; Nitsche, Benjamin M. ; Hondel, Cees A. van den ; Ram, Arthur F. ; Meyer, Vera (2016-11-11)

Understanding the genetic, molecular and evolutionary basis of cysteine-stabilized antifungal proteins (AFPs) from fungi is important for understanding whether their function is mainly defensive or associated with fungal growth and development. In the current study, a transcriptome meta-analysis of the Aspergillus niger γ-core protein AnAFP was performed to explore co-expressed genes and pathwa...

Universal law for diffusive mass transport through mycelial networks

Schmideder, Stefan ; Müller, Henri ; Barthel, Lars ; Friedrich, Tiaan ; Niessen, Ludwig ; Meyer, Vera ; Briesen, Heiko (2020-11-10)

Filamentous fungal cell factories play a pivotal role in biotechnology and circular economy. Hyphal growth and macroscopic morphology are critical for product titers; however, these are difficult to control and predict. Usually pellets, which are dense networks of branched hyphae, are formed during industrial cultivations. They are nutrient‐ and oxygen‐depleted in their core due to limited diff...

Mind the Fungi

Meyer, Vera ; Schmidt, Bertram ; Pohl, Carsten ; Cerimi, Kustrim ; Schubert, Bastian ; Weber, Birke ; Neubauer, Peter ; Junne, Stefan ; Zakeri, Zakieh ; Rapp, Regine ; Lutz, Christian de ; Schubert, Theresa ; Peluso, Fara ; Volpato, Alessandro (2020)

Dieses Buch berichtet über die Bündelung der Kreativitätsmotoren Wissenschaft und Kunst und wie daraus ein lebendiges Dreigespann aus Wissenschaft, Kunst und Gesellschaft geschmiedet werden kann. Eine schöpferische Triade, die sich über einen Zeitraum von zwei Jahren hinweg gemeinsam der Utopie verschrieben hat, eine Synthese aus nachhaltiger Wirtschaft, gesunder Umwelt und einer gerechten Gese...

From three‐dimensional morphology to effective diffusivity in filamentous fungal pellets

Schmideder, Stefan ; Barthel, Lars ; Müller, Henri ; Meyer, Vera ; Briesen, Heiko (2019-10-08)

Filamentous fungi are exploited as cell factories in biotechnology for the production of proteins, organic acids, and natural products. Hereby, fungal macromorphologies adopted during submerged cultivations in bioreactors strongly impact the productivity. In particular, fungal pellets are known to limit the diffusivity of oxygen, substrates, and products. To investigate the spatial distribution...

Comparative genomics of the aconidial Aspergillus niger strain LDM3 predicts genes associated with its high protein secretion capacity

Sui, Yu-Fei ; Ouyang, Li-Ming ; Schütze, Tabea ; Cheng, Shu ; Meyer, Vera ; Zhuang, Ying-Ping (2020-02-03)

Aspergillus niger is widely used as a cell factory for homologous and heterologous protein production. As previous studies reported that reduced sporulation favors protein secretion in A. niger, in this study, we conducted a comparative genomic analysis of the non-sporulating industrially exploited A. niger strain LDM3 in China and the reference protein secretion strain CBS 513.88 to predict th...

Multi-omics analysis of Aspergillus niger glucoamylase producing strains and exploration of NADPH metabolic engineering

Sui, Yufei (2020)

The filamentous fungus Aspergillus niger is one of the main cell factories used nowadays in the industry for homologous or heterologous protein production due to its extraordinary ability for protein expression and secretion. Systematic comprehension of its metabolic regulation characteristics during the fermentation process is of considerable significance to improve the performance of this ver...

Identifizierung einer Rezeptorbindestelle im Hüllprotein des Humanen Endogenen Retrovirus K(HML-2) und weitergehende Analysen der Interaktion des Hüllproteins mit zellulären Rezeptoren

Wamara, Jula (2020)

HERV-K(HML-2) ist das jüngste und am besten erhaltene humane endogene Retrovirus. Menschliche Zellen, die diese Viren alle im Genom tragen sind in der Lage funktionelle virale Hüllproteine zu exprimieren. Diese Proteine können andere Retroviren pseudotypisieren und ihren Zelltropismus dadurch verändern. Wichtige Charakteristika des Eintrittprozesses, der durch das Hüllprotein von HERV-K(HML-2) ...

Aspergillus niger Spores Are Highly Resistant to Space Radiation

Cortesão, Marta ; de Haas, Aram ; Unterbusch, Rebecca ; Fujimori, Akira ; Schütze, Tabea ; Meyer, Vera ; Möller, Ralf (2020-04-03)

The filamentous fungus Aspergillus niger is one of the main contaminants of the International Space Station (ISS). It forms highly pigmented, airborne spores that have thick cell walls and low metabolic activity, enabling them to withstand harsh conditions and colonize spacecraft surfaces. Whether A. niger spores are resistant to space radiation, and to what extent, is not yet known. In this st...

Herstellung und Charakterisierung von antimykotisch wirksamen Peptiden aus der AFP-Familie der Ascomycota

Paege, Norman (2020)

Die sehr variable und große Gruppe der antimikrobiellen Peptide, von der mehr als 10.000 Vertreter in der Datenbank „Collection of anti-microbial peptids“ CAMPR3 [1] gelistet sind, steht schon lange als potenzielle Basis für Wirkstoffe im Fokus der Forschung. Es gibt auf Grund steigender Zahlen von Pilzinfektionen und Resistenzen gegen antimykotische Wirkstoffe ein großes Bedürfnis diese Quelle...

Functional exploration of co-expression networks identifies a nexus for modulating protein and citric acid titres in Aspergillus niger submerged culture

Cairns, Timothy C. ; Feurstein, Claudia ; Zheng, Xiaomei ; Zhang, Li Hui ; Zheng, Ping ; Sun, Jibin ; Meyer, Vera (2019-11-09)

Background: Filamentous fungal cell factories are used to produce numerous proteins, enzymes, and organic acids. Protein secretion and filamentous growth are tightly coupled at the hyphal tip. Additionally, both these processes require ATP and amino acid precursors derived from the citric acid cycle. Despite this interconnection of organic acid production and protein secretion/filamentous growt...

Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

Kwon, Jin ; Schütze, Tabea ; Spohner, Sebastian ; Haefner, Stefan ; Meyer, Vera (2019-10-17)

Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a lot of efforts are invested to develop and improve protocols for the fungus and genes of interest with respect to applicability, scalability and targeting efficiencies. These efforts are often hampered by the fact th...

Species-Specific Differences in the Susceptibility of Fungi to the Antifungal Protein AFP Depend on C-3 Saturation of Glycosylceramides

Paege, Norman ; Warnecke, Dirk ; Zäuner, Simone ; Hagen, Silke ; Rodrigues, Ana ; Baumann, Birgit ; Thiess, Melanie ; Jung, Sascha ; Meyer, Vera (2019-12-11)

AFP is an antimicrobial peptide (AMP) produced by the filamentous fungus Aspergillus giganteus and is a very potent inhibitor of fungal growth that does not affect the viability of bacteria, plant, or mammalian cells. It targets chitin synthesis and causes plasma membrane permeabilization in many human- and plant-pathogenic fungi, but its exact mode of action is not known. After adoption of the...

A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger

Cairns, Timothy C. ; Feurstein, Claudia ; Zheng, Xiaomei ; Zheng, Ping ; Sun, Jibin ; Meyer, Vera (2019-06-15)

Background Fungal fermentation is used to produce a diverse repertoire of enzymes, chemicals, and drugs for various industries. During submerged cultivation, filamentous fungi form a range of macromorphologies, including dispersed mycelia, clumped aggregates, or pellets, which have critical implications for rheological aspects during fermentation, gas/nutrient transfer, and, thus, product titr...

Worms’ Antimicrobial Peptides

Bruno, Renato ; Maresca, Marc ; Canaan, Stéphane ; Cavalier, Jean-François ; Mabrouk, Kamel ; Boidin-Wichlacz, Céline ; Olleik, Hamza ; Zeppilli, Daniela ; Brodin, Priscille ; Massol, François ; Jollivet, Didier ; Jung, Sascha ; Tasiemski, Aurélie (2019-08-29)

Antimicrobial peptides (AMPs) are natural antibiotics produced by all living organisms. In metazoans, they act as host defense factors by eliminating microbial pathogens. But they also help to select the colonizing bacterial symbionts while coping with specific environmental challenges. Although many AMPs share common structural characteristics, for example having an overall size between 10–100...