FG Angewandte und Molekulare Mikrobiologie

8 Items

Recent Submissions
Uptake, physiological responses and technical application of the aquatic fungus Mucor hiemalis EH5 for the removal of cyanobacterial toxins

Balsano, Evelyn (2017)

Many cyanobacteria produce various harmful secondary metabolites, a.k.a. cyanotoxins, which cause global concerns because of their hazardousness to ecosystems and human health. Due to their diversity in origin and structure, cyanotoxins express different biological activities commonly including the generation of oxidative stress. This work focuses on the three most frequently occurring cyanotox...

From axenic to mixed cultures: technological advances accelerating a paradigm shift in microbiology

Meyer, Vera ; Nai, Corrado (2017)

Since the onset of microbiology in the late 19th century, scientists have been growing microorganisms almost exclusively as pure cultures, resulting in a limited and biased view of the microbial world. Only a paradigm shift in cultivation techniques – from axenic to mixed cultures – can allow a full comprehension of the (chemical) communication of microorganisms, with profound consequences for ...

Southern promises: a snapshot of the microbiology research landscape in South America based on bibliometric data

Nai, Corrado (2017-09)

Scientists have a single currency for productivity and impact: published articles. In an effort to map the global research landscape in microbiology, and to obviate the current lack of bibliometric analysis in the field, FEMS—the Federation of European Microbiological Societies—generated a dataset encompassing an exhaustive, worldwide list of microbiology studies for 2013–14, which further incl...

The induction and function of the transcription factor c-myc in macrophages during pneumococcal pneumonia and legionellosis

Kabus, Christin Sophia (2017)

For infectious diseases, effective defense mechanisms which constitute an efficient innate host response are the keys to eliminate invading pathogens. According to different studies, the pathogenicity of bacteria is due to different virulence factors that contribute to overcome those mechanisms. Against that, the immune system evolved a large repertoire to fight the pathogen. Nevertheless, an o...

Application and de novo design of artificial gene switches for Aspergillus niger

Schütze, Tabea (2017)

Aspergillus niger ist ein filamentöser Pilz, der industriell für die Produktion von organischen Säuren und Proteinen genutzt wird. In den letzten Jahren konnte darüber hinaus die Produktion von homo- und heterologen Sekundärmetaboliten gezeigt werden. Obwohl sich A. niger genetisch verändern lässt, gibt es nur wenig induzierbare Genexpressionssysteme. Außerdem ist die Ko-Expression von mehreren...

Engineering of Aspergillus niger for the production of secondary metabolites

Richter, Lennart ; Wanka, Franziska ; Boecker, Simon ; Storm, Dirk ; Kurt, Tutku ; Vural, Özlem ; Süßmuth, Roderich ; Meyer, Vera (2014)

Background: Filamentous fungi can each produce dozens of secondary metabolites which are attractive as therapeutics, drugs, antimicrobials, flavour compounds and other high-value chemicals. Furthermore, they can be used as an expression system for eukaryotic proteins. Application of most fungal secondary metabolites is, however, so far hampered by the lack of suitable fermentation protocols for...

Tet-on, or tet-off, that is the question: advanced conditional gene expression in Aspergillus

Wanka, Franziska ; Cairns, Timothy ; Boecker, Simon ; Berens, Christian ; Happel, Anna ; Zheng, Xiaomei ; Sun, Jibin ; Krappmann, Sven ; Meyer, Vera (2016)

In Aspergillus, controlled gene expression is often achieved using the reverse tetracycline-controlled transactivator (rtTA) dependent Tet-on system, whereby transcription is activated in a titratable manner by addition of the tetracycline derivative doxycycline. The complementary Tet-off system utilises the tetracycline-controlled transactivator (tTA) component to quantitatively reduce gene ex...

Gen- und verfahrenstechnische Ansätze zur Optimierung von Aspergillus niger als Expressionsplattform

Wanka, Franziska (2016)

Aspergillus niger has the ability to synthesise large amounts of acids and proteins, and to secrete these into the environment. This particular property is used in biotechnology to produce platform chemicals and industrial enzymes. Despite impressive metabolic activities, there is still a need for research to optimise product yields of A. niger using genetic and engineering approaches. This wo...