FG Bioverfahrenstechnik

24 Items

Recent Submissions
Modeling and simulation of biogas production based on anaerobic digestion of energy crops and manure

Arzate Salgado, Juan Antonio (2019)

Despite all knowledge related to AD, the complexity of the process leads to the fact that there exists still a lack of knowledge how the process will behave at disturbances and flexible feedstock utilization. At biogas plants, sampling and monitoring is in most cases not sufficient to detect disturbances in the process at an early stage. This lack of knowledge does not allow for a demand-driven...

Modelling overflow metabolism in Escherichia coli by acetate cycling

Anane, Emmanuel ; López Cárdenas, Diana Carolina ; Neubauer, Peter ; Cruz-Bournazou, M. Nicolas (2017-05-25)

A new set of mathematical equations describing overflow metabolism and acetate accumulation in E. coli cultivation is presented. The model is a significant improvement of already existing models in the literature, with modifications based on the more recent concept of acetate cycling in E. coli, as revealed by proteomic studies of overflow routes. This concept opens up new questions regarding t...

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Uusitalo, S. ; Kögler, Martin ; Välimaa, A.-L. ; Popov, A. ; Ryabchikov, Yu ; Kontturi, V. ; Siitonen, S. ; Petäjä, J. ; Virtanen, T. ; Laitinen, R. ; Kinnunen, M. ; Meglinski, I. ; Kabashin, A. ; Bunker, A. ; Viitala, T. ; Hiltunen, J. (2016)

The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been stu...

Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates

Knepper, Andreas ; Heiser, Michael ; Glauche, Florian ; Neubauer, Peter (2014)

The enormous variation possibilities of bioprocesses challenge process development to fix a commercial process with respect to costs and time. Although some cultivation systems and some devices for unit operations combine the latest technology on miniaturization, parallelization, and sensing, the degree of automation in upstream and downstream bioprocess development is still limited to single s...

Development of a High-Throughput Screening Assay Based on the 3-Dimensional Pannus Model for Rheumatoid Arthritis

Ibold, Yvonne ; Frauenschuh, Simone ; Kaps, Christian ; Sittinger, Michael ; Ringe, Jochen ; Goetz, Peter M. (2007)

The 3-dimensional (3-D) pannus model for rheumatoid arthritis (RA) is based on the interactive co-culture of cartilage and synovial fibroblasts (SFs). Besides the investigation of the pathogenesis of RA, it can be used to analyze the active profiles of antirheumatic pharmaceuticals and other bioactive substances under in vitro conditions. For a potential application in the industrial drug-scree...

Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification of Lactobacillus plantarum Cultures

Pellicer-Alborch, Klaus ; Angersbach, Alexander ; Neubauer, Peter ; Junne, Stefan (2018-12-04)

The rapid assessment of cell viability is crucial for process optimization, e.g., during media selection, determination of optimal environmental growth conditions and for quality control. In the present study, the cells' electric anisotropy of polarizability (AP) as well as the mean cell length in Lactobacillus plantarum batch and fed-batch fermentations were monitored with electrooptical measu...

Mobile multi-parameter measurements for the dynamic analysis of gradients in brewing vessels

Bockisch, Anika (2018)

Industrial processes have to be competitive and thus efficient. Products have to be produced with a high reproducibility and quality. At the same time, the scale of these processes reaches up to several 100 m³ and was even increasing in recent years. This is challenging since the power input is limited in large-scale processes. Therefore, mixing times are increasing in parallel to the volume, w...

Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform

Sawatzki, Annina ; Hans, Sebastian ; Narayanan, Harini ; Haby, Benjamin ; Krausch, Niels ; Sokolov, Michael ; Glauche, Florian ; Riedel, Sebastian L. ; Neubauer, Peter ; Cruz Bournazou, Mariano Nicolas (2018-11-21)

Mini-bioreactor systems enabling automatized operation of numerous parallel cultivations are a promising alternative to accelerate and optimize bioprocess development allowing for sophisticated cultivation experiments in high throughput. These include fed-batch and continuous cultivations with multiple options of process control and sample analysis which deliver valuable screening tools for ind...

Sequencing traces for plasmid pDR111

Giessmann, Robert T. (2018)

This data object contains the raw sequencing traces generated by Sanger dideoxy sequencing for the plasmid pDR111, a kind gift of David Z. Rudner.

Reconstruction of the lantibiotic ruminococcin-A biosynthesis machinery in Escherichia coli and structural characterization

Ongey, Elvis Legala (2018)

Lanthipeptides are a group of therapeutically relevant proteinaceous compounds predominantly produced by Gram-positive bacteria at the ribosomal level, containing thioether cross-linked amino acids called lanthionine as a characteristic structural element. Lanthipeptides are attracting growing interests stemming from their wide range of bioactivities including antimicrobial, antiviral and antic...

Heterologous biosynthesis, modifications and structural characterization of ruminococcin-A, a lanthipeptide from the gut bacterium Ruminococcus gnavus E1, in Escherichia coli

Ongey, Elvis Legala ; Giessmann, Robert ; Fons, Michel ; Rappsilber, Juri ; Adrian, Lorenz ; Neubauer, Peter (2018-07-26)

Ruminococcin A (RumA) is a lanthipeptide with high activity against pathogenic clostridia and is naturally produced by the strict anaerobic bacterium Ruminococcus gnavus E1, isolated from human intestine. Cultivating R. gnavus E1 is challenging, limiting high-quality production, further biotechnological development and therapeutic exploitation of RumA. To supply an alternative production system...

Bioinspired designs, molecular premise and tools for evaluating the ecological importance of antimicrobial peptides

Ongey, Elvis Legala ; Pflugmacher, Stephan ; Neubauer, Peter (2018-07-10)

This review article provides an overview of recent developments in antimicrobial peptides (AMPs), summarizing structural diversity, potential new applications, activity targets and microbial killing responses in general. The use of artificial and natural AMPs as templates for rational design of peptidomimetics are also discussed and some strategies are put forward to curtail cytotoxic effects a...

Real-time monitoring of the budding index in Saccharomyces cerevisiae batch cultivations with in situ microscopy

Marbà-Ardébol, Anna-Maria ; Emmerich, Jörn ; Muthig, Michael ; Neubauer, Peter ; Junne, Stefan (2018-05-15)

Background The morphology of yeast cells changes during budding, depending on the growth rate and cultivation conditions. A photo-optical microscope was adapted and used to observe such morphological changes of individual cells directly in the cell suspension. In order to obtain statistically representative samples of the population without the influence of sampling, in situ microscopy (ISM) w...

An observational study of ballooning in large spiders: Nanoscale multifibers enable large spiders’ soaring flight

Cho, Moonsung ; Neubauer, Peter ; Fahrenson, Christoph ; Rechenberg, Ingo (2018-06-14)

The physical mechanism of aerial dispersal of spiders, “ballooning behavior,” is still unclear because of the lack of serious scientific observations and experiments. Therefore, as a first step in clarifying the phenomenon, we studied the ballooning behavior of relatively large spiders (heavier than 5 mg) in nature. Additional wind tunnel tests to identify ballooning silks were implemented in t...

Growth rate dependent impact of oscillating glucose conditions on Escherichia coli physiology

Brand, Eva (2018)

Escherichia coli is the most used microorganism for biotechnological production processes due to its outstanding cultivation robustness and genetic and metabolic engineering possibilities. The established production process is a high cell density cultivation starting with a batch cultivation and followed by a fed-batch feeding strategy to avoid acetate accumulation and anaerobic conditions. The...

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Uusitalo, S. ; Kögler, Martin ; Välimaa, A.-L. ; Popov, A. ; Ryabchikov, Yu. ; Kontturi, V. ; Siitonen, S. ; Petäjä, J. ; Virtanen, T. ; Laitinen, R. ; Kinnunen, M. ; Meglinski, I. ; Kabashin, A. ; Bunker, A. ; Viitala, T. ; Hiltunen, J. (2016)

The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been stu...

Advanced Raman spectroscopy for bioprocess monitoring

Kögler, Martin (2018)

The Raman -effect was discovered almost 90 years ago. It took a long time until the importance of Raman spectroscopy was fully understood and accepted for process industrial applications. Still today the usage is limited to a small application area. During the last decades advances in the production of spectroscopic components have reduced the complexity of the instrumentation and further media...

Growth rate dependent impact of oscillating glucose conditions on Escherichia coli physiology: electronical supplement

Brand, Eva (2018)

Escherichia coli is the most used microorganism for biotechnological production processes due to its outstanding cultivation robustness and genetic and metabolic engineering possibilities. The established production process is a high cell density cultivation starting with a batch cultivation and followed by a fed-batch feeding strategy to avoid acetate accumulation and anaerobic conditions. The...

Application of the enzyme controlled glucose feeding for the production of recombinant proteins

Abdou Ali, Basant (2018)

One of the prime objectives of any bioprocess development is to enhance the microbial growth and product yields. Correspondingly, for recombinant proteins it is the aim increase the yields of the soluble, active and correctly folded molecule. There are many factors that could influence the product yield like medium optimization and the performance of the bioprocess itself. The aim of this study...

Micro-electromechanical affinity sensor for the monitoring of glucose in bioprocess media

Theuer, Lorenz ; Lehmann, Micha ; Junne, Stefan ; Neubauer, Peter ; Birkholz, Mario (2017)

An affinity-viscometry-based micro-sensor probe for continuous glucose monitoring was investigated with respect to its suitability for bioprocesses. The sensor operates with glucose and dextran competing as binding partner for concanavalin A, while the viscosity of the assay scales with glucose concentration. Changes in viscosity are determined with a micro-electromechanical system (MEMS) in th...