Uusitalo, S. ; Kögler, Martin ; Välimaa, A.-L. ; Popov, A. ; Ryabchikov, Yu. ; Kontturi, V. ; Siitonen, S. ; Petäjä, J. ; Virtanen, T. ; Laitinen, R. ; Kinnunen, M. ; Meglinski, I. ; Kabashin, A. ; Bunker, A. ; Viitala, T. ; Hiltunen, J. (2016)
The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been stu...Advanced Raman spectroscopy for bioprocess monitoring
Kögler, Martin (2018)
The Raman -effect was discovered almost 90 years ago. It took a long time until the importance of Raman spectroscopy was fully understood and accepted for process industrial applications. Still today the usage is limited to a small application area. During the last decades advances in the production of spectroscopic components have reduced the complexity of the instrumentation and further media...Growth rate dependent impact of oscillating glucose conditions on Escherichia coli physiology: electronical supplement
Brand, Eva (2018)
Escherichia coli is the most used microorganism for biotechnological production processes due to its outstanding cultivation robustness and genetic and metabolic engineering possibilities. The established production process is a high cell density cultivation starting with a batch cultivation and followed by a fed-batch feeding strategy to avoid acetate accumulation and anaerobic conditions. The...Application of the enzyme controlled glucose feeding for the production of recombinant proteins
Abdou Ali, Basant (2018)
One of the prime objectives of any bioprocess development is to enhance the microbial growth and product yields. Correspondingly, for recombinant proteins it is the aim increase the yields of the soluble, active and correctly folded molecule. There are many factors that could influence the product yield like medium optimization and the performance of the bioprocess itself. The aim of this study...Micro-electromechanical affinity sensor for the monitoring of glucose in bioprocess media
Theuer, Lorenz ; Lehmann, Micha ; Junne, Stefan ; Neubauer, Peter ; Birkholz, Mario (2017)
An affinity-viscometry-based micro-sensor probe for continuous glucose monitoring was investigated with respect to its suitability for bioprocesses. The sensor operates with glucose and dextran competing as binding partner for concanavalin A, while the viscosity of the assay scales with glucose concentration. Changes in viscosity are determined with a micro-electromechanical system (MEMS) in th...Platform technologies for automated bioprocess development
Glauche, Florian (2018)
The development of production processes in biotechnology is a time and resource intensive task due to the vast design space to be screened. Therefore, processes are commonly performed at a local optimum. Within the past two decades, miniaturization, parallelization and automation of laboratory work have improved significantly and researchers are now able to carry out thousands of experiments pe...Impacts of oscillating cultivation conditions on the quality of recombinant inclusion bodies in Escherichia coli
Reitz, Christian (2017)
The controlled use of microorganisms in the pharmaceutical, (bio-)chemical and life science industries to produce recombinant proteins or other organic compounds in large-scale bioreactors is a standard procedure today. However, predicting the process performance after scaling up from the development scale to the final production scale is still a critical problem in modern bioprocess developmen...Toward microbioreactor arrays
Glauche, Florian ; John, Gernot T. ; Arain, Sarina ; Knepper, Andreas ; Neubauer, Antje ; Goelling, Detlef ; Lang, Christine ; Violet, Norman ; King, Rudibert ; Neubauer, Peter (2015)
In this study, a slow-responding chemo-optical sensor for dissolved oxygen (DO) integrated into a 96-well plate was developed. The slow response time ensures that the measured oxygen value does not change much during plate transport to the microplate reader. The sensor therefore permits at-line DO measurement of microbial cultures. Moreover, it eliminates the necessity of individual optical mea...Impact of oscillations in substrate and oxygen availability on Corynebacterium glutamicum cultivations
Lemoine, Anja Irene (2017)
Corynebacterium glutamicum is a microorganism which is highly relevant for industrial large scale applications. Nowadays, it is mainly used for amino acid production in the food and feed industry in a scale of several hundred cubic meters. Furthermore, it is a promising candidate for the production of bulk chemicals such as the biopolymer precursor Cadaverine. Gradient formation occurs in indus...
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