FG Bioverfahrenstechnik

35 Items

Recent Submissions
Chemoenzymatic synthesis of nucleoside analogs as potential medicinal agents

Mohamed, Heba (2019)

Modified nucleosides are important drugs used to treat cancer, viral or bacterial infections. They also serve as precursors for the synthesis of modified oligonucleotides (antisense oligonucleotides (ASOs) or short interfering RNAs (siRNAs)), a novel and effective class of therapeutics. While the chemical synthesis of nucleoside analogs is challenging due to multi-step procedures and low select...

Downstream processing of lactic acid fermentation broth from renewable feed stocks

Hendrik, Laube (2019)

Der Schwerpunkt dieser Arbeit liegt auf der Aufreinigung von Milchsäure aus Fermentationslösungen als Ausgangsstoff für die Polymerchemie. Dabei kommt der chemischen Prozessanalyse als Ausgangspunkt für die Evaluierung der jeweiligen Effektivität des Prozessschrittes eine wichtige Rolle zu. Es wurde eine Kapillarelektrophorese-Methode mittels UV/VIS-Detektor entwickelt, welche neben Milchsäure ...

Is Ghana Ready to Attain Sustainable Development Goal (SDG) Number 7?—A Comprehensive Assessment of Its Renewable Energy Potential and Pitfalls

Acheampong, Michael ; Yu, Qiuyan ; Cansu Ertem, Funda ; Deba Enomah Ebude, Lucy ; Tanim, Shakhawat ; Eduful, Michael ; Vaziri, Mehrdad ; Ananga, Erick (2019-01-28)

Ghana has declared support for the UN Sustainable Development Goal (SDG) number seven which most importantly target ensuring universal access to affordable, reliable and modern energy services. This target presents a formidable challenge to Ghana because the country still relies mainly on traditional biomass as its primary source of energy coupled with a chronically fragile hydropower sector. I...

Life cycle assessment and modeling approaches as a combined evaluation tool for sustainable control strategies at biogas plants

Ertem-Kappler, Funda Cansu (2019)

Anaerobic digestion and biogas production can help to meet several targets concerning energy and environmental policy. However, biogas generation is a complex process where many challenges need to be tackled to achieve real sustainable operation. In this regard, better control, monitoring and optimization strategies need to be implemented. This thesis focuses on the combination of Life Cycle As...

Quantitative and sensitive RNA based detection of Bacillus spores

Osmekhina, Ekaterina ; Shvetsova, Antonina ; Ruottinen, Maria ; Neubauer, Peter (2014-03-11)

The fast and reliable detection of bacterial spores is of great importance and still remains a challenge. Here we describe a direct RNA-based diagnostic method for the specific detection of viable bacterial spores which does not depends on an enzymatic amplification step and therefore is directly appropriate for quantification. The procedure includes the following steps: (i) heat activation of ...

A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

Kaspar, Felix ; Giessmann, Robert T. ; Krausch, Niels ; Neubauer, Peter ; Wagner, Anke ; Gimpel, Matthias (2019-07-15)

Efficient reaction monitoring is crucial for data acquisition in kinetic and mechanistic studies. However, for conversions of nucleosides to their corresponding nucleobases, as observed in enzymatically catalyzed nucleoside phosphorylation reactions, the current analytical arsenal does not meet modern requirements regarding cost, speed of analysis and high throughput. Herein, we present a UV/Vi...

Reproduction of Large-Scale Bioreactor Conditions on Microfluidic Chips

Ho, Phuong ; Westerwalbesloh, Christoph ; Kaganovitch, Eugen ; Grünberger, Alexander ; Neubauer, Peter ; Kohlheyer, Dietrich ; Lieres, Eric von (2019-04-19)

Microbial cells in industrial large-scale bioreactors are exposed to fluctuating conditions, e.g., nutrient concentration, dissolved oxygen, temperature, and pH. These inhomogeneities can influence the cell physiology and metabolism, e.g., decelerate cell growth and product formation. Microfluidic systems offer new opportunities to study such effects in great detail by examining responses to va...

Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates

Hans, Sebastian ; Gimpel, Matthias ; Glauche, Florian ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2018-06-25)

Metabolic engineering and genome editing strategies often lead to large strain libraries of a bacterial host. Nevertheless, the generation of competent cells is the basis for transformation and subsequent screening of these strains. While preparation of competent cells is a standard procedure in flask cultivations, parallelization becomes a challenging task when working with larger libraries an...

Monte Carlo Simulations for the Analysis of Non-linear Parameter Confidence Intervals in Optimal Experimental Design

Krausch, Niels ; Barz, Tilman ; Sawatzki, Annina ; Gruber, Mathis ; Kamel, Sarah ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2019-05-24)

Especially in biomanufacturing, methods to design optimal experiments are a valuable technique to fully exploit the potential of the emerging technical possibilities that are driving experimental miniaturization and parallelization. The general objective is to reduce the experimental effort while maximizing the information content of an experiment, speeding up knowledge gain in R&D. The approac...

Dynamic Modelling of Phosphorolytic Cleavage Catalyzed by Pyrimidine-Nucleoside Phosphorylase

Giessmann, Robert T. ; Krausch, Niels ; Kaspar, Felix ; Cruz-Bournazou, Mariano Nicolas ; Wagner, Anke ; Neubauer, Peter ; Gimpel, Matthias (2019-06-19)

Pyrimidine-nucleoside phosphorylases (Py-NPases) have a significant potential to contribute to the economic and ecological production of modified nucleosides. These can be produced via pentose-1-phosphates, an interesting but mostly labile and expensive precursor. Thus far, no dynamic model exists for the production process of pentose-1-phosphates, which involves the equilibrium state of the Py...

CFD-basierte evolutions­strategische Black-Box-Optimierung zur Abschätzung des Potentials des Bionikpropellers im realen Einsatz am Schiff

Schmid, Michael (2019)

Die Evolution hat in der Natur viele Lösungen unter dem besonderen Gesichtspunkt der Energieeffizienz hervorgebracht. Dabei sind insbesondere bei Vögeln interessante Lösungen entstanden, die sich im Sinne der Bionik als effizienzsteigernde Maßnamen verstehen und technisch umsetzen lassen. Der bei der Auftriebserzeugung am Vogel- bzw. Tragflügel entstehende Spitzenwirbel ist unvermeidlich, der E...

Modeling and simulation of biogas production based on anaerobic digestion of energy crops and manure

Arzate Salgado, Juan Antonio (2019)

Despite all knowledge related to AD, the complexity of the process leads to the fact that there exists still a lack of knowledge how the process will behave at disturbances and flexible feedstock utilization. At biogas plants, sampling and monitoring is in most cases not sufficient to detect disturbances in the process at an early stage. This lack of knowledge does not allow for a demand-driven...

Modelling overflow metabolism in Escherichia coli by acetate cycling

Anane, Emmanuel ; López Cárdenas, Diana Carolina ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2017-05-25)

A new set of mathematical equations describing overflow metabolism and acetate accumulation in E. coli cultivation is presented. The model is a significant improvement of already existing models in the literature, with modifications based on the more recent concept of acetate cycling in E. coli, as revealed by proteomic studies of overflow routes. This concept opens up new questions regarding t...

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Uusitalo, S. ; Kögler, Martin ; Välimaa, A.-L. ; Popov, A. ; Ryabchikov, Yu ; Kontturi, V. ; Siitonen, S. ; Petäjä, J. ; Virtanen, T. ; Laitinen, R. ; Kinnunen, M. ; Meglinski, I. ; Kabashin, A. ; Bunker, A. ; Viitala, T. ; Hiltunen, J. (2016)

The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. Current bacteria identification practices, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been stu...

Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates

Knepper, Andreas ; Heiser, Michael ; Glauche, Florian ; Neubauer, Peter (2014)

The enormous variation possibilities of bioprocesses challenge process development to fix a commercial process with respect to costs and time. Although some cultivation systems and some devices for unit operations combine the latest technology on miniaturization, parallelization, and sensing, the degree of automation in upstream and downstream bioprocess development is still limited to single s...

Development of a High-Throughput Screening Assay Based on the 3-Dimensional Pannus Model for Rheumatoid Arthritis

Ibold, Yvonne ; Frauenschuh, Simone ; Kaps, Christian ; Sittinger, Michael ; Ringe, Jochen ; Goetz, Peter M. (2007)

The 3-dimensional (3-D) pannus model for rheumatoid arthritis (RA) is based on the interactive co-culture of cartilage and synovial fibroblasts (SFs). Besides the investigation of the pathogenesis of RA, it can be used to analyze the active profiles of antirheumatic pharmaceuticals and other bioactive substances under in vitro conditions. For a potential application in the industrial drug-scree...

Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification of Lactobacillus plantarum Cultures

Pellicer-Alborch, Klaus ; Angersbach, Alexander ; Neubauer, Peter ; Junne, Stefan (2018-12-04)

The rapid assessment of cell viability is crucial for process optimization, e.g., during media selection, determination of optimal environmental growth conditions and for quality control. In the present study, the cells' electric anisotropy of polarizability (AP) as well as the mean cell length in Lactobacillus plantarum batch and fed-batch fermentations were monitored with electrooptical measu...

Mobile multi-parameter measurements for the dynamic analysis of gradients in brewing vessels

Bockisch, Anika (2018)

Industrial processes have to be competitive and thus efficient. Products have to be produced with a high reproducibility and quality. At the same time, the scale of these processes reaches up to several 100 m³ and was even increasing in recent years. This is challenging since the power input is limited in large-scale processes. Therefore, mixing times are increasing in parallel to the volume, w...

Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform

Sawatzki, Annina ; Hans, Sebastian ; Narayanan, Harini ; Haby, Benjamin ; Krausch, Niels ; Sokolov, Michael ; Glauche, Florian ; Riedel, Sebastian L. ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2018-11-21)

Mini-bioreactor systems enabling automatized operation of numerous parallel cultivations are a promising alternative to accelerate and optimize bioprocess development allowing for sophisticated cultivation experiments in high throughput. These include fed-batch and continuous cultivations with multiple options of process control and sample analysis which deliver valuable screening tools for ind...

Sequencing traces for plasmid pDR111

Giessmann, Robert T. (2018)

This data object contains the raw sequencing traces generated by Sanger dideoxy sequencing for the plasmid pDR111, a kind gift of David Z. Rudner.