FG Bioverfahrenstechnik

60 Items

Recent Submissions
Monitoring Parallel Robotic Cultivations with Online Multivariate Analysis

Hans, Sebastian ; Ulmer, Christian ; Narayanan, Harini ; Brautaset, Trygve ; Krausch, Niels ; Neubauer, Peter ; Schäffl, Irmgard ; Sokolov, Michael ; Cruz-Bournazou, Mariano Nicolas (2020-05-14)

In conditional microbial screening, a limited number of candidate strains are tested at different conditions searching for the optimal operation strategy in production (e.g., temperature and pH shifts, media composition as well as feeding and induction strategies). To achieve this, cultivation volumes of >10 mL and advanced control schemes are required to allow appropriate sampling and analyses...

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR

Nejati, Fatemeh ; Junne, Stefan ; Kurreck, Jens ; Neubauer, Peter (2020-06-16)

Kefir grains are complex microbial systems of several groups of microorganisms. The identification and quantification of the microbial composition of milk kefirs was described in several studies, which provided an insight into the microbial consortia in this complex ecosystem. Nevertheless, the current methods for identification and quantification are not appropriate for deeper studies on kefir...

Ansätze zur Optimierung der Glutathionausbeute in der Hefe Saccharomyces spp.

Lorenz, Eric (2020-02-05)

Glutathione is the most abundant non-protein thiol compound and tripeptide found in eukaryotes. The primary function of glutathione is to maintain the redox status and to protect the cell against oxidation caused by production of e.g. radicals or environmental influences. It is subject of different studies due to its physiological importance and industrial relevance. Based on its anti-oxidative...

Separation, Characterization, and Handling of Microalgae by Dielectrophoresis

Abt, Vinzenz ; Gringel, Fabian ; Han, Arum ; Neubauer, Peter ; Birkholz, Mario (2020-04-09)

Microalgae biotechnology has a high potential for sustainable bioproduction of diverse high-value biomolecules. Some of the main bottlenecks in cell-based bioproduction, and more specifically in microalgae-based bioproduction, are due to insufficient methods for rapid and efficient cell characterization, which contributes to having only a few industrially established microalgal species in comme...

Simulation of the point-mass-loaded filament in the homogeneous turbulence

Cho, Moonsung (2020-01-14)

The passive dynamics of a ballooning spider in the chaotic flow motion of atmosphere is important to understand its suspension capability. To model a ballooning silk that is suspended in the air flow, we introduce the bead-spring model. Each bead is connected to the other with a tensile and a bending spring. By adjusting each stiffness value, we can simulate various extensibility and flexibilit...

Visualisation of hairpin vortex packets in the near-ground atmospheric boundary layer during daytime

Cho, Moonsung (2020-01-14)

Visualisation of the flow structure near the ground is important to understand aerial locomotion of a passive flying animal. The video shows the visualized structures of several hairpin vortex packets in the near ground atmospheric boundary layer during daytime. The hairpin vortex packets appear as a wedge-like shape. The structure develops upwards, as it drifts in the downwind direction. (Mean...

A crab spider’s ballooning process

Cho, Moonsung (2020-01-14)

A crab spider’s ballooning process is recorded in the field condition on an artificial dome. The video is converted to negative video to visualize ballooning lines. The video contains initial phase of spinning ballooning lines, fluttering of a bundle of ballooning lines by turbulent wind flows, take-off moment and the scene of airborne state of a ballooning spider.

Suspension of a point-mass-loaded filament in non-uniform flows: The ballooning flight of spiders

Cho, Moonsung (2020)

Some spiders can disperse through the air using their fine silk filaments. This is a technique referred to as ‘ballooning’. Not only from an ecological point of view, but also from the perspective of physics, spiders’ ballooning is an interesting phenomenon, as it relates to the question of how a large particle can disperse long distances through a fluctuating fluid medium (in this case, the Ea...

Single-Use Printed Biosensor for L-Lactate and Its Application in Bioprocess Monitoring

Theuer, Lorenz ; Randek, Judit ; Junne, Stefan ; Neubauer, Peter ; Mandenius, Carl-Fredrik ; Beni, Valerio (2020-03-09)

There is a profound need in bioprocess manufacturing for low-cost single-use sensors that allow timely monitoring of critical product and production attributes. One such opportunity is screen-printed enzyme-based electrochemical sensors, which have the potential to enable low-cost online and/or off-line monitoring of specific parameters in bioprocesses. In this study, such a single-use electroc...

A Big World in Small Grain: A Review of Natural Milk Kefir Starters

Nejati, Fatemeh ; Junne, Stefan ; Neubauer, Peter (2020-01-30)

Milk kefir is a traditional fermented milk product whose consumption is becoming increasingly popular. The natural starter for kefir production is kefir grain, which consists of various bacterial and yeast species. At the industrial scale, however, kefir grains are rarely used due to their slow growth, complex application, bad reproducibility and high costs. Instead, mixtures of defined lactic ...

Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations

Yehia, Heba ; Westarp, Sarah ; Röhrs, Viola ; Kaspar, Felix ; Giessmann, Robert T. ; Klare, Hendrik F. T. ; Paulick, Katharina ; Neubauer, Peter ; Kurreck, Jens ; Wagner, Anke (2020-02-19)

The enzymatic synthesis of nucleoside analogues has been shown to be a sustainable and efficient alternative to chemical synthesis routes. In this study, dihalogenated nucleoside analogues were produced by thermostable nucleoside phosphorylases in transglycosylation reactions using uridine or thymidine as sugar donors. Prior to the enzymatic process, ideal maximum product yields were calculated...

Enzymatic lignocellulosic feedstock degradation and lactic acid production by Bacillus coagulans

Glaser, Robert (2020)

For an operator of a lignocellulosic biorefinery plant, simple and fast methods are needed to reproducibly define and optimise processes. For the development of enzymes, methods are required which allow for the assessment of the effectiveness of cellulase as close to the process as possible, and which guarantee quick and easy technology transfers to the industry. Currently, quality parameters a...

Thermodynamic reaction control of nucleoside phosphorolysis

Kaspar, Felix ; Giessmann, Robert T. ; Neubauer, Peter ; Wagner, Anke ; Gimpel, Matthias (2020-01-07)

Nucleoside analogs represent a class of important drugs for cancer and antiviral treatments. Nucleoside phosphorylases (NPases) catalyze the phosphorolysis of nucleosides and are widely employed for the synthesis of pentose‐1‐phosphates and nucleoside analogs, which are difficult to access via conventional synthetic methods. However, for the vast majority of nucleosides, it has been observed th...

Adaptive optimal operation of a parallel robotic liquid handling station

Barz, Tilman ; Sommer, Andreas ; Wilms, Terrance ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2018-05-03)

Results are presented from the optimal operation of a fully automated robotic liquid handling station where parallel experiments are performed for calibrating a kinetic fermentation model. To increase the robustness against uncertainties and/or wrong assumptions about the parameter values, an iterative calibration and experiment design approach is adopted. Its implementation yields a stepwise r...

Model reduction of aerobic bioprocess models for efficient simulation

Duan, Zhaoyang ; Wilms, Terrance ; Neubauer, Peter ; Kravaris, Costas ; Cruz-Bournazou, Mariano Nicolas (2020-02-14)

Owing to the increasing demand for large scale and high efficiency in manufacturing processes, computer aided tools for process operation and control are rapidly gaining popularity. An important state variable in aerobic processes is the dissolved oxygen, which can be easily measured online and is an important indicator of the metabolic activity. However, due to the fast kinetics of the oxygen ...

Bioprocess Development for Lantibiotic Ruminococcin-A Production in Escherichia coli and Kinetic Insights Into LanM Enzymes Catalysis

Ongey, Elvis L. ; Santolin, Lara ; Waldburger, Saskia ; Adrian, Lorenz ; Riedel, Sebastian L. ; Neubauer, Peter (2019-09-13)

Ruminococcin-A (RumA) is a peptide antibiotic with post-translational modifications including thioether cross-links formed from non-canonical amino acids, called lanthionines, synthesized by a dedicated lanthionine-generating enzyme RumM. RumA is naturally produced by Ruminococcus gnavus, which is part of the normal bacterial flora in the human gut. High activity of RumA against pathogenic Clos...

Fungi as source for new bio-based materials: a patent review

Cerimi, Kustrim ; Akkaya, Kerem Can ; Pohl, Carsten ; Schmidt, Bertram ; Neubauer, Peter (2019-10-26)

Background The circular economy closes loops in industrial manufacturing processes and minimizes waste. A bio-based economy aims to replace fossil-based resources and processes by sustainable alternatives which exploits renewable biomass for the generation of products used in our daily live. A current trend in fungal biotechnology—the production of fungal-based biomaterials—will contribute to ...

A model‐based framework for parallel scale‐down fed‐batch cultivations in mini‐bioreactors for accelerated phenotyping

Anane, Emmanuel ; García, Ángel Córcoles ; Haby, Benjamin ; Hans, Sebastian ; Krausch, Niels ; Krewinkel, Manuel ; Hauptmann, Peter ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2019-07-30)

Concentration gradients that occur in large industrial‐scale bioreactors due to mass transfer limitations have significant effects on process efficiency. Hence, it is desirable to investigate the response of strains to such heterogeneities to reduce the risk of failure during process scale‐up. Although there are various scale‐down techniques to study these effects, scale‐down strategies are rar...

Human Deoxycytidine Kinase Is a Valuable Biocatalyst for the Synthesis of Nucleotide Analogues

Hellendahl, Katja F. ; Kamel, Sarah ; Wetterwald, Albane ; Neubauer, Peter ; Wagner, Anke (2019-11-27)

Natural ribonucleoside-5’-monophosphates are building blocks for nucleic acids which are used for a number of purposes, including food additives. Their analogues, additionally, are used in pharmaceutical applications. Fludarabine-5´-monophosphate, for example, is effective in treating hematological malignancies. To date, ribonucleoside-5’-monophosphates are mainly produced by chemical synthesis...

Enzymatic Synthesis of alpha-D-Pentofuranose -1- Phosphate as a universal glycosyl donor for modified nucleosides synthesis using thermostable nucleoside phosphorylase

Kamel Hassan, Sarah (2019)

⍺-D-pentofuranose-1-phosphate is a key molecule in the pentose phosphate pathway as well as in the nucleoside/nucleotide metabolic pathway. It is physiologically synthesized from D-pentoses by phosphopentomutase or from nucleosides by nucleoside phosphorylase. It is further used as a carbon and energy source via its utilization in glycolysis and/or re-entering the pentose phosphate pathway. Pen...