FG Bioverfahrenstechnik

85 Items

Recent Submissions
Editorial: Continuous Biomanufacturing in Microbial Systems

Herwig, Christoph ; Slouka, Christoph ; Neubauer, Peter ; Delvigne, Frank (2021-06-07)

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Analytical methods and thermodynamic frameworks for efficient biocatalytic nucleoside synthesis via nucleoside phosphorylases

Kaspar, Felix (2021)

Nucleosides and nucleoside analogs are indispensable biomolecules which serve as buildings blocks of DNA and RNA, pharmaceuticals and molecular biology tools. Sparked by the demand for synthetic access to these compounds, a variety of approaches for nucleoside synthesis has been developed. Among these, biocatalytic nucleoside synthesis via nucleoside phosphorylases promises a solution to the lo...

The Nonribosomal Peptide Valinomycin: From Discovery to Bioactivity and Biosynthesis

Huang, Shuhui ; Liu, Yushi ; Liu, Wan-Qiu ; Neubauer, Peter ; Li, Jian (2021-04-08)

Valinomycin is a nonribosomal peptide that was discovered from Streptomyces in 1955. Over the past more than six decades, it has received continuous attention due to its special chemical structure and broad biological activities. Although many research papers have been published on valinomycin, there has not yet been a comprehensive review that summarizes the diverse studies ranging from struct...

Substrate-Flexible Two-Stage Fed-Batch Cultivations for the Production of the PHA Copolymer P(HB-co-HHx) With Cupriavidus necator Re2058/pCB113

Santolin, Lara ; Waldburger, Saskia ; Neubauer, Peter ; Riedel, Sebastian L. (2021-03-22)

Recent studies of the impact and dimension of plastic pollution have drawn the attention to finding more sustainable alternatives to fossil-based plastics. Microbially produced polyhydroxyalkanoates (PHAs) biopolymers are strong candidates to replace conventional plastic materials, due to their true biodegradability and versatile properties. However, widespread use of these polymers is still hi...

Low-quality animal by-product streams for the production of PHA-biopolymers: fats, fat/protein-emulsions and materials with high ash content as low-cost feedstocks

Saad, Victoria ; Gutschmann, Björn ; Grimm, Thomas ; Widmer, Torsten ; Neubauer, Peter ; Riedel, Sebastian L. (2020-12-26)

Objective: The rapid accumulation of crude-oil based plastics in the environment is posing a fundamental threat to the future of mankind. The biodegradable and bio-based polyhydroxyalkanoates (PHAs) can replace conventional plastics, however, their current production costs are not competitive and therefore prohibiting PHAs from fulfilling their potential. Results: Different low-quality animal b...

Route efficiency assessment and review of the synthesis of β-nucleosides via N-glycosylation of nucleobases

Kaspar, Felix ; Stone, M. Rhia L. ; Neubauer, Peter ; Kurreck, Anke (2020-11-19)

Nucleosides and their analogs are biomolecules central to nearly all areas of life science. Consequently, a variety of approaches have been developed to prepare these compounds. These methods typically employ N-glycosylation as a key step which installs a sugar moiety on a heterocyclic nucleobase. However, these methods vary drastically regarding their synthetic strategy, number of steps, yield...

Anaerobic treatment of liquid by-products from hydrothermal carbonization of biomass

Wirth, Benjamin (2021)

Hydrothermal carbonization (HTC) is a promising technology to convert highly wet biogenic residues into a solid product similar to lignite. Most studies focus on maximizing the solid yield and on various solid product applications. However, as HTC takes place in water, intermediates dissolve in the liquid phase resulting in a carbon loss of the feedstock leaving behind a highly contaminated pro...

Kinetic Analysis of the Hydrolysis of Pentose‐1‐phosphates through Apparent Nucleoside Phosphorolysis Equilibrium Shifts

Kaspar, Felix ; Neubauer, Peter ; Kurreck, Anke (2020-12-04)

Herein, we report an addition to the toolbox for the monitoring and quantification of the hydrolytic decay of pentose‐1‐phosphates, which are known to be elusive and difficult to quantify. This communication describes how apparent equilibrium shifts of a nucleoside phosphorolysis reaction can be employed to calculate hydrolytic loss of pentose‐1‐phosphates based on the measurement of post‐hydro...

Comparative metalloproteomic study in anaerobically respiring microorganisms

Budhraja, Rohit (2021)

Metal ions are essential for many biological processes in the microbial system and have structural, catalytic or electron-transferring roles. Metal ions are well organized in membrane-associated respiration protein complexes where they couple electron transport and proton translocation across the membrane to generate energy. These metalloproteins generate an electrically conductive path in the ...

Heterologous hydrogenase overproduction systems for biotechnology—An overview

Fan, Qin ; Neubauer, Peter ; Lenz, Oliver ; Gimpel, Matthias (2020-08-16)

Hydrogenases are complex metalloenzymes, showing tremendous potential as H2-converting redox catalysts for application in light-driven H2 production, enzymatic fuel cells and H2-driven cofactor regeneration. They catalyze the reversible oxidation of hydrogen into protons and electrons. The apo-enzymes are not active unless they are modified by a complicated post-translational maturation process...

Automated Conditional Screening of Multiple Escherichia coli Strains in Parallel Adaptive Fed-Batch Cultivations

Hans, Sebastian ; Haby, Benjamin ; Krausch, Niels ; Barz, Tilman ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2020-11-11)

In bioprocess development, the host and the genetic construct for a new biomanufacturing process are selected in the early developmental stages. This decision, made at the screening scale with very limited information about the performance in larger reactors, has a major influence on the efficiency of the final process. To overcome this, scale-down approaches during screenings that show the rea...

Spiral microfluidic devices for cell separation and sorting in bioprocesses

Herrmann, Niklas ; Neubauer, Peter ; Birkholz, Mario (2019-11-05)

Inertial microfluidic systems have been arousing interest in medical applications due to their simple and cost-efficient use. However, comparably small sample volumes in the microliter and milliliter ranges have so far prevented efficient applications in continuous bioprocesses. Nevertheless, recent studies suggest that these systems are well suited for cell separation in bioprocesses because o...

A Genome-Scale Insight into the Effect of Shear Stress During the Fed-Batch Production of Clavulanic Acid by Streptomyces Clavuligerus

Gómez-Ríos, David ; López-Agudelo, Victor A. ; Ramírez-Malule, Howard ; Neubauer, Peter ; Junne, Stefan ; Ochoa, Silvia ; Ríos-Estepa, Rigoberto (2020-08-19)

Streptomyces clavuligerus is a filamentous Gram-positive bacterial producer of the β-lactamase inhibitor clavulanic acid. Antibiotics biosynthesis in the Streptomyces genus is usually triggered by nutritional and environmental perturbations. In this work, a new genome scale metabolic network of Streptomyces clavuligerus was reconstructed and used to study the experimentally observed effect of o...

Molecular genetic approaches to decrease mis-incorporation of non-canonical branched chain amino acids into a recombinant protein in Escherichia coli

Córcoles García, Ángel (2020)

The incorporation of non-canonical branched chain amino acids (ncBCAA) such as norleucine, norvaline and ß-methylnorleucine into recombinant proteins during E.coli production processes has become a crucial matter of contention in the pharmaceutical industry, since such mis-incorporation can lead to production of altered proteins, having non optimal characteristics. Hence, a need exists for nove...

Identification of the Unwinding Region in the Clostridioides difficile Chromosomal Origin of Replication

Oliveira Paiva, Ana M. ; van Eijk, Erika ; Friggen, Annemieke H. ; Weigel, Christoph ; Smits, Wiep Klaas (2020-10-02)

Faithful DNA replication is crucial for viability of cells across all kingdoms. Targeting DNA replication is a viable strategy for inhibition of bacterial pathogens. Clostridioides difficile is an important enteropathogen that causes potentially fatal intestinal inflammation. Knowledge about DNA replication in this organism is limited and no data is available on the very first steps of DNA repl...

Recovery of the PHA Copolymer P(HB-co-HHx) With Non-halogenated Solvents: Influences on Molecular Weight and HHx-Content

Bartels, Moritz ; Gutschmann, Björn ; Widmer, Torsten ; Grimm, Thomas ; Neubauer, Peter ; Riedel, Sebastian L. (2020-08-07)

Biodegradable and biocompatible polyhydroxyalkanoates (PHAs) are promising alternatives to conventional plastics. Based on the chain length of their monomers they are classified as short chain length (scl-) or medium chain length (mcl-) PHA polymers. The type of monomers, the composition and the molecular weight (MW) define the polymer properties. To accelerate the use of PHA as a bulk material...

An Approach to Ring Resonator Biosensing Assisted by Dielectrophoresis: Design, Simulation and Fabrication

Henriksson, Anders ; Kasper, Laura ; Jäger, Matthias ; Neubauer, Peter ; Birkholz, Mario (2020-10-22)

The combination of extreme miniaturization with a high sensitivity and the potential to be integrated in an array form on a chip has made silicon-based photonic microring resonators a very attractive research topic. As biosensors are approaching the nanoscale, analyte mass transfer and bonding kinetics have been ascribed as crucial factors that limit their performance. One solution may be a sys...

Role of Microbial Hydrolysis in Anaerobic Digestion

Menzel, Theresa ; Neubauer, Peter ; Junne, Stefan (2020-10-23)

There is a growing need of substrate flexibility for biobased production of energy and value-added products that allows the application of variable biodegradable residues within a circular economy. It can be used to balance fluctuating energy provision of other renewable sources. Hydrolysis presents one of the biggest limitations during anaerobic digestion. Methods to improve it will result in ...

Adaptive Monitoring of Biotechnological Processes Kinetics

Lyubenova, Velislava ; Ignatova, Maya ; Roeva, Olympia ; Junne, Stefan ; Neubauer, Peter (2020-10-17)

In this paper, an approach for the monitoring of biotechnological process kinetics is proposed. The kinetics of each process state variable is presented as a function of two time-varying unknown parameters. For their estimation, a general software sensor is derived with on-line measurements as inputs that are accessible in practice. The stability analysis with a different number of inputs shows...

Model based optimization of transflection near infrared spectroscopy as a process analytical tool in a continuous flash pasteurizer

Weishaupt, Imke ; Zimmer, Manuel ; Neubauer, Peter ; Schneider, Jan (2020-06-29)

Near infrared spectroscopy in combination with a transflection probe was investigated as inline measurement in a continuous flash pasteurizer system with a sugar–water model solution. Robustness and reproducibility of fluctuations of recorded spectra as well as trueness of the chemometric analysis were compared under different process parameter settings. Variable parameters were the flow rate (...