FG Bioverfahrenstechnik

45 Items

Recent Submissions
Bioprocess Development for Lantibiotic Ruminococcin-A Production in Escherichia coli and Kinetic Insights Into LanM Enzymes Catalysis

Ongey, Elvis L. ; Santolin, Lara ; Waldburger, Saskia ; Adrian, Lorenz ; Riedel, Sebastian L. ; Neubauer, Peter (2019-09-13)

Ruminococcin-A (RumA) is a peptide antibiotic with post-translational modifications including thioether cross-links formed from non-canonical amino acids, called lanthionines, synthesized by a dedicated lanthionine-generating enzyme RumM. RumA is naturally produced by Ruminococcus gnavus, which is part of the normal bacterial flora in the human gut. High activity of RumA against pathogenic Clos...

Fungi as source for new bio-based materials: a patent review

Cerimi, Kustrim ; Akkaya, Kerem Can ; Pohl, Carsten ; Schmidt, Bertram ; Neubauer, Peter (2019-10-26)

Background The circular economy closes loops in industrial manufacturing processes and minimizes waste. A bio-based economy aims to replace fossil-based resources and processes by sustainable alternatives which exploits renewable biomass for the generation of products used in our daily live. A current trend in fungal biotechnology—the production of fungal-based biomaterials—will contribute to ...

A model‐based framework for parallel scale‐down fed‐batch cultivations in mini‐bioreactors for accelerated phenotyping

Anane, Emmanuel ; García, Ángel Córcoles ; Haby, Benjamin ; Hans, Sebastian ; Krausch, Niels ; Krewinkel, Manuel ; Hauptmann, Peter ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2019-07-30)

Concentration gradients that occur in large industrial‐scale bioreactors due to mass transfer limitations have significant effects on process efficiency. Hence, it is desirable to investigate the response of strains to such heterogeneities to reduce the risk of failure during process scale‐up. Although there are various scale‐down techniques to study these effects, scale‐down strategies are rar...

Human Deoxycytidine Kinase Is a Valuable Biocatalyst for the Synthesis of Nucleotide Analogues

Hellendahl, Katja F. ; Kamel, Sarah ; Wetterwald, Albane ; Neubauer, Peter ; Wagner, Anke (2019-11-27)

Natural ribonucleoside-5’-monophosphates are building blocks for nucleic acids which are used for a number of purposes, including food additives. Their analogues, additionally, are used in pharmaceutical applications. Fludarabine-5´-monophosphate, for example, is effective in treating hematological malignancies. To date, ribonucleoside-5’-monophosphates are mainly produced by chemical synthesis...

Enzymatic Synthesis of alpha-D-Pentofuranose -1- Phosphate as a universal glycosyl donor for modified nucleosides synthesis using thermostable nucleoside phosphorylase

Kamel Hassan, Sarah (2019)

⍺-D-pentofuranose-1-phosphate is a key molecule in the pentose phosphate pathway as well as in the nucleoside/nucleotide metabolic pathway. It is physiologically synthesized from D-pentoses by phosphopentomutase or from nucleosides by nucleoside phosphorylase. It is further used as a carbon and energy source via its utilization in glycolysis and/or re-entering the pentose phosphate pathway. Pen...

Characterization of the Metabolic Response of Streptomyces clavuligerus to Shear Stress in Stirred Tanks and Single-Use 2D Rocking Motion Bioreactors for Clavulanic Acid Production

Gómez-Ríos, David ; Junne, Stefan ; Neubauer, Peter ; Ochoa, Silvia ; Ríos-Estepa, Rigoberto ; Ramírez-Malule, Howard (2019-09-27)

Streptomyces clavuligerus is a gram-positive filamentous bacterium notable for producing clavulanic acid (CA), an inhibitor of β-lactamase enzymes, which confers resistance to bacteria against several antibiotics. Here we present a comparative analysis of the morphological and metabolic response of S. clavuligerus linked to the CA production under low and high shear stress conditions in a 2D ro...

In-Line Monitoring of Polyhydroxyalkanoate (PHA) Production during High-Cell-Density Plant Oil Cultivations Using Photon Density Wave Spectroscopy

Gutschmann, Björn ; Schiewe, Thomas ; Weiske, Manon T.H. ; Neubauer, Peter ; Hass, Roland ; Riedel, Sebastian L. (2019-09-19)

Polyhydroxyalkanoates (PHAs) are biodegradable plastic-like materials with versatile properties. Plant oils are excellent carbon sources for a cost-effective PHA production, due to their high carbon content, large availability, and comparatively low prices. Additionally, efficient process development and control is required for competitive PHA production, which can be facilitated by on-line or ...

Modelling concentration gradients in fed‐batch cultivations of E. coli – towards the flexible design of scale‐down experiments

Anane, Emmanuel ; Sawatzki, Annina ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2018-08-13)

BACKGROUND: The impact of concentration gradients in large industrial-scale bioreactors on microbial physiology can be studied in scale-down bioreactors. However, scale-down systems pose several challenges in construction, operation and footprint. Therefore, it is challenging to implement them in emerging technologies for bioprocess development, such as in high throughput cultivation platforms....

Spiral microfluidic devices for cell separation and sorting in bioprocesses

Herrmann, Niklas ; Neubauer, Peter ; Birkholz, Mario (2019-11-05)

Inertial microfluidic systems have been arousing interest in medical applications due to their simple and cost-efficient use. However, comparably small sample volumes in the microliter and milliliter ranges have so far prevented efficient applications in continuous bioprocesses. Nevertheless, recent studies suggest that these systems are well suited for cell separation in bioprocesses because o...

Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Peebo, Karl ; Neubauer, Peter (2018-06-21)

Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines the output of a recombinant protein producing system, having control over specific growth rate o...

Chemoenzymatic synthesis of nucleoside analogs as potential medicinal agents

Mohamed, Heba (2019)

Modified nucleosides are important drugs used to treat cancer, viral or bacterial infections. They also serve as precursors for the synthesis of modified oligonucleotides (antisense oligonucleotides (ASOs) or short interfering RNAs (siRNAs)), a novel and effective class of therapeutics. While the chemical synthesis of nucleoside analogs is challenging due to multi-step procedures and low select...

Downstream processing of lactic acid fermentation broth from renewable feed stocks

Laube, Hendrik (2019)

Der Schwerpunkt dieser Arbeit liegt auf der Aufreinigung von Milchsäure aus Fermentationslösungen als Ausgangsstoff für die Polymerchemie. Dabei kommt der chemischen Prozessanalyse als Ausgangspunkt für die Evaluierung der jeweiligen Effektivität des Prozessschrittes eine wichtige Rolle zu. Es wurde eine Kapillarelektrophorese-Methode mittels UV/VIS-Detektor entwickelt, welche neben Milchsäure ...

Is Ghana Ready to Attain Sustainable Development Goal (SDG) Number 7?—A Comprehensive Assessment of Its Renewable Energy Potential and Pitfalls

Acheampong, Michael ; Yu, Qiuyan ; Cansu Ertem, Funda ; Deba Enomah Ebude, Lucy ; Tanim, Shakhawat ; Eduful, Michael ; Vaziri, Mehrdad ; Ananga, Erick (2019-01-28)

Ghana has declared support for the UN Sustainable Development Goal (SDG) number seven which most importantly target ensuring universal access to affordable, reliable and modern energy services. This target presents a formidable challenge to Ghana because the country still relies mainly on traditional biomass as its primary source of energy coupled with a chronically fragile hydropower sector. I...

Life cycle assessment and modeling approaches as a combined evaluation tool for sustainable control strategies at biogas plants

Ertem-Kappler, Funda Cansu (2019)

Anaerobic digestion and biogas production can help to meet several targets concerning energy and environmental policy. However, biogas generation is a complex process where many challenges need to be tackled to achieve real sustainable operation. In this regard, better control, monitoring and optimization strategies need to be implemented. This thesis focuses on the combination of Life Cycle As...

Quantitative and sensitive RNA based detection of Bacillus spores

Osmekhina, Ekaterina ; Shvetsova, Antonina ; Ruottinen, Maria ; Neubauer, Peter (2014-03-11)

The fast and reliable detection of bacterial spores is of great importance and still remains a challenge. Here we describe a direct RNA-based diagnostic method for the specific detection of viable bacterial spores which does not depends on an enzymatic amplification step and therefore is directly appropriate for quantification. The procedure includes the following steps: (i) heat activation of ...

A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

Kaspar, Felix ; Giessmann, Robert T. ; Krausch, Niels ; Neubauer, Peter ; Wagner, Anke ; Gimpel, Matthias (2019-07-15)

Efficient reaction monitoring is crucial for data acquisition in kinetic and mechanistic studies. However, for conversions of nucleosides to their corresponding nucleobases, as observed in enzymatically catalyzed nucleoside phosphorylation reactions, the current analytical arsenal does not meet modern requirements regarding cost, speed of analysis and high throughput. Herein, we present a UV/Vi...

Reproduction of Large-Scale Bioreactor Conditions on Microfluidic Chips

Ho, Phuong ; Westerwalbesloh, Christoph ; Kaganovitch, Eugen ; Grünberger, Alexander ; Neubauer, Peter ; Kohlheyer, Dietrich ; Lieres, Eric von (2019-04-19)

Microbial cells in industrial large-scale bioreactors are exposed to fluctuating conditions, e.g., nutrient concentration, dissolved oxygen, temperature, and pH. These inhomogeneities can influence the cell physiology and metabolism, e.g., decelerate cell growth and product formation. Microfluidic systems offer new opportunities to study such effects in great detail by examining responses to va...

Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates

Hans, Sebastian ; Gimpel, Matthias ; Glauche, Florian ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2018-06-25)

Metabolic engineering and genome editing strategies often lead to large strain libraries of a bacterial host. Nevertheless, the generation of competent cells is the basis for transformation and subsequent screening of these strains. While preparation of competent cells is a standard procedure in flask cultivations, parallelization becomes a challenging task when working with larger libraries an...

Monte Carlo Simulations for the Analysis of Non-linear Parameter Confidence Intervals in Optimal Experimental Design

Krausch, Niels ; Barz, Tilman ; Sawatzki, Annina ; Gruber, Mathis ; Kamel, Sarah ; Neubauer, Peter ; Cruz-Bournazou, Mariano Nicolas (2019-05-24)

Especially in biomanufacturing, methods to design optimal experiments are a valuable technique to fully exploit the potential of the emerging technical possibilities that are driving experimental miniaturization and parallelization. The general objective is to reduce the experimental effort while maximizing the information content of an experiment, speeding up knowledge gain in R&D. The approac...

Dynamic Modelling of Phosphorolytic Cleavage Catalyzed by Pyrimidine-Nucleoside Phosphorylase

Giessmann, Robert T. ; Krausch, Niels ; Kaspar, Felix ; Cruz-Bournazou, Mariano Nicolas ; Wagner, Anke ; Neubauer, Peter ; Gimpel, Matthias (2019-06-19)

Pyrimidine-nucleoside phosphorylases (Py-NPases) have a significant potential to contribute to the economic and ecological production of modified nucleosides. These can be produced via pentose-1-phosphates, an interesting but mostly labile and expensive precursor. Thus far, no dynamic model exists for the production process of pentose-1-phosphates, which involves the equilibrium state of the Py...