Fakultät 3 Prozesswissenschaften

1018 Items

Recent Submissions
Application of the enzyme controlled glucose feeding for the production of recombinant proteins

Abdou Ali, Basant (2018)

One of the prime objectives of any bioprocess development is to enhance the microbial growth and product yields. Correspondingly, for recombinant proteins it is the aim increase the yields of the soluble, active and correctly folded molecule. There are many factors that could influence the product yield like medium optimization and the performance of the bioprocess itself. The aim of this study...

Micro-electromechanical affinity sensor for the monitoring of glucose in bioprocess media

Theuer, Lorenz ; Lehmann, Micha ; Junne, Stefan ; Neubauer, Peter ; Birkholz, Mario (2017)

An affinity-viscometry-based micro-sensor probe for continuous glucose monitoring was investigated with respect to its suitability for bioprocesses. The sensor operates with glucose and dextran competing as binding partner for concanavalin A, while the viscosity of the assay scales with glucose concentration. Changes in viscosity are determined with a micro-electromechanical system (MEMS) in th...

Biologisch-chemische Behandlung von Prozeßwässern der Altlastensanierung sowie von kontaminierten Grundwässern

Behrendt, Joachim (1993)

An einer vierstufigen Laborversuchsanlage, stehend aus einem Airlift-Schlaufensuspensionsreaktor (ASSR), einem Festbettumlaufreaktor (FBR 1), einem Schlaufenreaktor, der mit ozonhaltigem Sauerstoff begast wurde, (O3R) und einem weiteren Festbettumlaufreaktor (FBR 2), wurden Versuche zum Abbau und zur chemischen Oxidation mit nachfolgendem biologischen Abbau von Inhaltsstoffen aus Prozeßwässern ...

Platform technologies for automated bioprocess development

Glauche, Florian (2018)

The development of production processes in biotechnology is a time and resource intensive task due to the vast design space to be screened. Therefore, processes are commonly performed at a local optimum. Within the past two decades, miniaturization, parallelization and automation of laboratory work have improved significantly and researchers are now able to carry out thousands of experiments pe...

Uptake, physiological responses and technical application of the aquatic fungus Mucor hiemalis EH5 for the removal of cyanobacterial toxins

Balsano, Evelyn (2017)

Many cyanobacteria produce various harmful secondary metabolites, a.k.a. cyanotoxins, which cause global concerns because of their hazardousness to ecosystems and human health. Due to their diversity in origin and structure, cyanotoxins express different biological activities commonly including the generation of oxidative stress. This work focuses on the three most frequently occurring cyanotox...

RNA-Interferenz-basierte Therapien bei Adenovirusinfektionen im immunsupprimierten Wirt

Schaar, Katrin (2017)

Infektionen mit humanen Adenoviren verlaufen meist mild und sind nach wenigen Wochen auskuriert. Vor allem die oberen Atemwege sind betroffen, abhängig vom Serotypen können sich auch andere Symptome ergeben. Liegt eine weitere Erkrankung vor, die mit einer natürlichen oder pharmakolo-gisch induzierten Schwächung des Immunsystems einhergeht, z.B. nach Transplantationsprozeduren, verlaufen die Er...

Role of autoantigen-specific T cell subsets in disease pathogenesis and as therapeutic tool in systemic lupus erythematosus

Abdirama, Dimas (2017)

Systemic lupus erythematosus (SLE) is a chronic and systemic autoimmune disorder characterized by loss of immune tolerance towards nuclear antigens such as SmD1, RNP70, Histone, Ro, and La. Besides the production of autoantibodies that directed to nuclear antigens, CD4+ T cells with T cell receptor specific to nuclear antigens may also trigger autoimmune responses leading to severe organ inflam...

Impacts of oscillating cultivation conditions on the quality of recombinant inclusion bodies in Escherichia coli

Reitz, Christian (2017)

The controlled use of microorganisms in the pharmaceutical, (bio-)chemical and life science industries to produce recombinant proteins or other organic compounds in large-scale bioreactors is a standard procedure today. However, predicting the process performance after scaling up from the development scale to the final production scale is still a critical problem in modern bioprocess developmen...

Tribologisch induzierte Oberflächenveränderungen im Reib-Verschleiß-Kontakt Kolbenring gegen Zylinderlaufbahn

Biberger, Julian (2017)

In Verbrennungsmotoren ist der Tribokontakt Topring gegen Zylinderlaufbahn einer der am höchsten beanspruchten Kontakte. Die Anforderungen an Reibungsoptimierung und geringen Verschleiß bei gleichzeitig hoher Laufzeit erfordern ein detailliertes Verständnis dieses tribologischen Kontaktes. Aus diesem Grund ist es erforderlich, unterschiedliche Kolbenringbeschichtungen auf ihr tribologisches Ver...

Systematic coalescence investigations in liquid/liquid systems

Kamp, Johannes (2017)

Liquid/liquid dispersions, which are mixtures of (nearly) immiscible liquids, are an integral part of many technical processes and everyday consumer products. For example, in technical processes the interfacial area is increased by dispersion to enhance mass and heat transfer; or consumer products like lotions and creams consist of stabilised oil/water dispersions. The process efficiency and re...

From axenic to mixed cultures: technological advances accelerating a paradigm shift in microbiology

Meyer, Vera ; Nai, Corrado (2017)

Since the onset of microbiology in the late 19th century, scientists have been growing microorganisms almost exclusively as pure cultures, resulting in a limited and biased view of the microbial world. Only a paradigm shift in cultivation techniques – from axenic to mixed cultures – can allow a full comprehension of the (chemical) communication of microorganisms, with profound consequences for ...

Die Schädigung des unreifen Kleinhirns durch Hyperoxie in Ratten als Modell der Hirnschädigung bei Frühgeborenen

Scheuer, Till (2017)

Former preterm infants often suffer from impaired postnatal brain development. In general, brain injury is reported to be caused by hypoxia/ischemia, infection/inflammation and hyperoxia. Due to recent insights, pathologies of the cerebellum can often be found, too. Preterm birth seems to be associated with a reduction of the cerebellar volume and decreased cell density in the cerebellar cortex...

Entwicklung von HPLC-MS/MS-Methoden zum Nachweis von proteinbasierten Bindungssystemen in Fleisch und Fleischerzeugnissen

Jira, Wolfgang (2017)

Im Rahmen der vorliegenden Arbeit wurden auf Basis der Kopplung von Hochleistungsflüssigchromatographie und Tandem-Massenspektrometrie (HPLC-MS/MS) basierende Methoden zum Nachweis des Einsatzes der marktrelevanten proteinbasierten Bindungssysteme mikrobielle Transglutaminase (TG) und Fibrinogen/Thrombin (FT) von Rind und Schwein bei Fleisch und rohen Fleischerzeugnissen entwickelt. Für die Er...

Production of extracellular DNA (eDNA) of the γ-Proteobacterium Rheinheimera sp. F8 in biofilms

Schuster, Anna-Kathrin (2017)

Abstract F8 is a gram-negative γ-Proteobacterium, which belongs to the genus Rheinheimera. The strain has been isolated from the South Saskatchewan River in Canada (Böckelmann, 2002). It has been discovered in previous studies that F8 releases DNA into the extracellular space (Böckelmann et al., 2006; Böckelmann et al., 2007). The aim of this study was a more detailed analysis of the F8 extrace...

Automated universal chip platform for fluorescence based cellular assays

Schmieder, F. ; Schmieder, S. ; Eger, R. ; Friedrich, S. ; Werner, A. ; Danz, N. ; Marx, Uwe ; Sonntag, Frank (2012)

The advantage of cell based assays used as biosensors is the direct access to hardly obtainable parameters like toxicity, mutagenicity and pharmacological effectiveness. Within the last few years we established a micro fluidic platform including a peristaltic micro pump as well as several valves, manifolds and micro channels [1]. For optical online monitoring the micro fluidic system is bonded ...

Hollow fibres integrated in a microfluidic cell culture system

Winkelmann, Claudia ; Luo, Y. ; Lode, A. ; Gelinsky, M. ; Marx, Uwe ; Sonntag, Frank (2012)

For in vitro drug screening a modified perfusion micro-bioreactor system with integrated hollow fibres will be demonstrated. This biocompatible system consists of an integrated closed flow circuit that includes reservoirs and pneumatic micro pumps. Additional optical online-monitoring devices allow the observation during the cell cultivation. The embedded hollow fibre system which acts as cell ...

Automated Micro-PIV measurement in Lab-on-a-Chip systems

Busek, Mathias ; Polk, Christoph ; Albrecht, Thomas ; Marx, Uwe ; König, J. ; Sonntag, Frank (2012)

Flow rate and wall shear stress are important parameters for perfused cell culture systems and should be monitored. An easy and non-invasive method is the particle image velocimetry (PIV). In this work PIV was used to characterize a cell culture system with included peristaltic pump. The time-dependent flow profile was measured on several points of the chip for different pumping speeds to figur...

Ultramicroporous silicon nitride ceramics for CO2 capture

Schitco, Cristina ; Seifollahi Bazarjani, Mahdi ; Riedel, Ralf ; Gurlo, Aleksander (2015)

Carbon dioxide (CO2) capture is regarded as one of the biggest challenges of the 21st century; therefore, intense research effort has been dedicated in the area of developing new materials for efficient CO2 capture. Here, we report high CO2 capture capacity in the low region of applied CO2 pressures observed with ultramicroporous silicon nitride-based material. The latter is synthesized by a fa...

Suitability of various complex hydrides for foaming aluminum alloys

Kamm, Paul H. ; García-Moreno, Francisco ; Jiménez, Catalina ; Banhart, John (2013)

Some hydrides that could replace TiH2 as the hitherto most suitable blowing agent for foaming aluminum alloys were investigated. Hydrides taken from the group MBH4 (M = Li, Na, K) and LiAlH4 were selected since these have not been studied in the past although their decomposition characteristics appear to be suitable. Foamable precursors of alloy AlSi8Mg4 were manufactured by pressing blends of ...

Etablierung und Charakterisierung eines dreidimensionalen Zellkulturmodells für Kuhpockeninfektionen

Koban, Robert (2017)

Zellkultivierungen stellen ein elementares Werkzeug für die Erforschung vielfältiger biologischer Phänomene dar. In der Virologie werden Infektionen in vitro fast ausschließlich in Monolayer-Kulturen charakterisiert und erforscht, in denen Zellen flach ausgebreitet auf starren Oberflächen kultiviert werden und im Vergleich zum in vivo Status der gleichen Zellen deutliche Unterschiede bezüglich ...