FG Biologische Chemie

8 Items

Recent Submissions
The Natural Fungal Metabolite Beauvericin Exerts Anticancer Activity In Vivo: A Pre-Clinical Pilot Study

Heilos, Daniela ; Rodríguez-Carrasco, Yelko ; Englinger, Bernhard ; Timelthaler, Gerald ; Van Schoonhoven, Sushilla ; Sulyok, Michael ; Boecker, Simon ; Süßmuth, Roderich D. ; Heffeter, Petra ; Lemmens-Gruber, Rosa ; Dornetshuber-Fleiss, Rita ; Berger, Walter (2017-08-24)

Recently, in vitro anti-cancer properties of beauvericin, a fungal metabolite were shown in various cancer cell lines. In this study, we assessed the specificity of this effect by comparing beauvericin cytotoxicity in malignant versus non-malignant cells. Moreover, we tested in vivo anticancer effects of beauvericin by treating BALB/c and CB-17/SCID mice bearing murine CT-26 or human KB-3-1-gra...

Untersuchungen zur Biosynthese von Omphalotin

Ramm, Sascha (2019)

Originally discovered in 1987, Omphalotin is an undodecapeptide (which is) synthesized by the basidiomycete Omphalotus olearius. The fungal secondary metabolite shows a strong nematicide and selective activity against the plant parasite Meloidogyne incognita. Structurally, it bears various remarkable characteristics, as for example nine methylations of the α-nitrogen atoms in the peptide backbo...

Application of a Rapid and Integrated Analysis System (RIAS) as a High-Throughput Processing Tool for In Vitro ADME Samples by Liquid Chromatography/Tandem Mass Spectrometry

Luippold, Andreas H. ; Arnhold, Thomas ; Jörg, Wolfgang ; Krüger, Beate ; Süßmuth, Roderich D. (2011)

Over the past decade, drug discovery programs have started to address the optimization of key ADME properties already at an early stage of the process. Hence, analytical chemists have been confronted with tremendously rising sample numbers and have had to develop methodologies accelerating quantitative liquid chromatography/tandem mass spectrometry (LC/MS/MS). This article focuses on the applic...

Characteristic Features of the Regulatory Functions of the ᴅ-Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase from Spinach

Vater, Joachim ; Gaudszun, Thomas ; Lange, Benita ; Erdin, Nazmiye ; Salnikow, Johann (1983)

Catalysis and regulation of CO2 fixation differ in a characteristic manner in their response to anionic modifiers and the polarity of the reaction medium. Monovalent inorganic anions inhibit catalysis and CO2-activation of the ᴅ-ribulose 1,5-bisphosphate carboxylase/oxygenase from spinach, whereas the activity and binding of NADPH and effector sugar phosphates are affected only at appreciably h...

Comprehensive snake venomics of the Okinawa Habu pit viper, Protobothrops flavoviridis, by complementary mass spectrometry-guided approaches

Damm, Maik ; Hempel, Benjamin-Florian ; Nalbantsoy, Ayse ; Süßmuth, Roderich D. (2018-07-29)

The Asian world is home to a multitude of venomous and dangerous snakes, which are used to induce various medical effects in the preparation of traditional snake tinctures and alcoholics, like the Japanese snake wine, named Habushu. The aim of this work was to perform the first quantitative proteomic analysis of the Protobothrops flavoviridis pit viper venom. Accordingly, the venom was analyzed...

Comparative venomics of the Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni from Turkey provides insights into kinship

Hempel, Benjamin-Florian ; Damm, Maik ; Göçmen, Bayram ; Karis, Mert ; Oguz, Mehmet Anıl ; Nalbantsoy, Ayse ; Süßmuth, Roderich D. (2018)

The Nose-horned Viper (Vipera ammodytes) is one of the most widespread and venomous snakes in Europe, which causes high frequent snakebite accidents. The first comprehensive venom characterization of the regional endemic Transcaucasian Nose-horned Viper (Vipera ammodytes transcaucasiana) and the Transdanubian Sand Viper (Vipera ammodytes montandoni) is reported employing a combination of intact...

Synthese von fluoreszierenden 7-Azatryptophan-Derivaten und deren Einbau in Modellpeptide

Noichl, Benjamin Philipp (2017)

In nature, proteins evolve to ensure the survival of living organisms in their natural environments. To equip them with new features and functions, classical protein engineering, based on canonical twenty amino acids, could lead to the improvement of these proteins by mutagenesis. Proteins sequences containing exclusively naturally occurring amino acids (i.e. canonical 20) are often unsuitable ...

Biological effects of paenilamicin, a secondary metabolite antibiotic produced by the honey bee pathogenic bacterium Paenibacillus larvae

Garcia-Gonzalez, Eva ; Müller, Sebastian ; Hertlein, Gillian ; Heid, Nina ; Süßmuth, Roderich D. ; Genersch, Elke (2014)

Paenibacillus larvae is the etiological agent of American Foulbrood (AFB) a world-wide distributed devastating disease of the honey bee brood. Previous comparative genome analysis and more recently, the elucidation of the bacterial genome, provided evidence that this bacterium harbors putative functional nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) and therefore, mig...