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Main Title: Xenomicrobiology: a roadmap for genetic code engineering
Author(s): Acevedo-Rocha, Carlos G.
Budisa, Nediljko
Type: Article
Language Code: en
Abstract: Biology is an analytical and informational science that is becoming increasingly dependent on chemical synthesis. One example is the high-throughput and low-cost synthesis of DNA, which is a foundation for the research field of synthetic biology (SB). The aim of SB is to provide biotechnological solutions to health, energy and environmental issues as well as unsustainable manufacturing processes in the frame of naturally existing chemical building blocks. Xenobiology (XB) goes a step further by implementing nonnatural building blocks in living cells. In this context, genetic code engineering respectively enables the redesign of genes/genomes and proteins/proteomes with non-canonical nucleic (XNAs) and amino (ncAAs) acids. Besides studying information flow and evolutionary innovation in living systems, XB allows the development of new-to-nature therapeutic proteins/ peptides, new biocatalysts for potential applications in synthetic organic chemistry and biocontainment strategies for enhanced biosafety. In this perspective, we provide a brief history and evolution of the genetic code in the context of XB. We then discuss the latest efforts and challenges ahead for engineering the genetic code with focus on substitutions and additions of ncAAs as well as standard amino acid reductions. Finally, we present a roadmap for the directed evolution of artificial microbes for emancipating rare sense codons that could be used to introduce novel building blocks. The development of such xenomicroorganisms endowed with a 'genetic firewall' will also allow to study and understand the relation between code evolution and horizontal gene transfer.
Issue Date: 2016
Date Available: 14-Jul-2017
DDC Class: 570 Biowissenschaften; Biologie
610 Medizin und Gesundheit
Subject(s): noncanonical amino-acids
escherichia coli cells
transfer RNA
modified organisms
synthetic biology
Journal Title: Microbial biotechnology
Publisher: Wiley-Blackwell
Publisher Place: Oxford
Volume: 9
Issue: 5
Publisher DOI: 10.1111/1751-7915.12398
Page Start: 666
Page End: 676
EISSN: 1751-7915
ISSN: 1751-7907
Appears in Collections:FG Biokatalyse » Publications

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