Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-6007
Main Title: Evolution of fluorinated enzymes: an emerging trend for biocatalyst stabilization
Author(s): Biava, Hernan
Budisa, Nediljko
Type: Article
Language Code: en
Abstract: Nature uses remarkably limited sets of chemistries in its repertoire, especially when compared to synthetic organic chemistry. This limits both the chemical and structural diversity that can ultimately be achieved with biocatalysis, unless the powers of chemical synthesis are merged with biological systems by integrating nonnatural synthetic chemistries into the protoplasma of living cells. Of particular interest, here is the fluorous effect that has recently established the potential to generate enzymes with an increased resistance toward both high temperature and organic solvents. For these reasons, we are witnessing a rapid development of efficient methodologies for the incorporation of fluorinated amino acids in protein synthesis, using both in vivo and in vitro strategies. In this review, we highlight relevant and trendsetting results in the design and engineering of stable fluorinated proteins and peptides along with whole-cell biocatalysis as an economically attractive and convenient application with exclusive focus on industrial biocatalysis. Finally, we envision new strategies to improve current achievements and enable the field to progress far beyond the current state-of-the-art.
URI: http://depositonce.tu-berlin.de/handle/11303/6499
http://dx.doi.org/10.14279/depositonce-6007
Issue Date: 2014
Date Available: 14-Jul-2017
DDC Class: 660 Technische Chemie
Subject(s): biocatalysis
enzyme stabilization
fluorinated amino acids
fluorination
fluorous effect
Sponsor/Funder: DFG, EXC 314, Unifying Concepts in Catalysis
Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/
Journal Title: Engineering in life sciences
Publisher: Wiley-VCH
Publisher Place: Weinheim
Volume: 14
Issue: 4
Publisher DOI: 10.1002/elsc.201300049
Page Start: 340
Page End: 351
EISSN: 1618-2863
ISSN: 1618-0240
Appears in Collections:Technische Universität Berlin » Fakultäten & Zentralinstitute » Fakultät 2 Mathematik und Naturwissenschaften » Institut für Chemie » Fachgebiet Biokatalyse » Publications

Files in This Item:
File Description SizeFormat 
10.1002.elsc.201300049.pdf959.76 kBAdobe PDFThumbnail
View/Open


Items in DepositOnce are protected by copyright, with all rights reserved, unless otherwise indicated.