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Main Title: Proteomics of a fuzzy organelle: interphase chromatin
Author(s): Kustatscher, Georg
Hégarat, Nadia
Wills, Karen L. H.
Furlan, Cristina
Bukowski-Wills, Jimi-Carlo
Hochegger, Helfrid
Rappsilber, Juri
Type: Article
Language Code: en
Abstract: Chromatin proteins mediate replication, regulate expression, and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle, it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead, we propose a new approach that provides a quantitative assessment of a protein's probability to function in chromatin. We integrate chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including 1840 previously uncharacterized proteins. We demonstrate the power of our large-scale data-driven annotation during the analysis of cyclin-dependent kinase (CDK) regulation in chromatin. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology.
Issue Date: 2014
Date Available: 14-Jul-2017
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): Cdk regulation
machine learning
Journal Title: The EMBO journal
Publisher: Wiley
Publisher Place: Hoboken, NJ [u.a.]
Volume: 33
Issue: 6
Publisher DOI: 10.1002/embj.201387614
Page Start: 648
Page End: 664
EISSN: 1460-2075
ISSN: 0261-4189
Appears in Collections:FG Bioanalytik » Publications

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