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Main Title: Toward microbioreactor arrays
Subtitle: a slow-responding xxygen sensor for monitoring of microbial cultures in standard 96-well plates
Author(s): Glauche, Florian
John, Gernot T.
Arain, Sarina
Knepper, Andreas
Neubauer, Antje
Goelling, Detlef
Lang, Christine
Violet, Norman
King, Rudibert
Neubauer, Peter
Type: Article
Language Code: en
Abstract: In this study, a slow-responding chemo-optical sensor for dissolved oxygen (DO) integrated into a 96-well plate was developed. The slow response time ensures that the measured oxygen value does not change much during plate transport to the microplate reader. The sensor therefore permits at-line DO measurement of microbial cultures. Moreover, it eliminates the necessity of individual optical measurement systems for each culture plate, as many plates can be measured successively. Combined with the 96-well format, this increases the experimental throughput enormously. The novel sensor plate (Slow OxoPlate) consists of fluorophores suspended in a polymer matrix that were placed into u-bottom 96-well plates. Response time was measured using sodium sulfite, and a t90 value of 9.7 min was recorded. For application, DO values were then measured in Escherichia coli and Saccharomyces cerevisiae cultures grown under fed-batch–like conditions. Depending on the DO sensor’s response time, different information on the oxygenation state of the culture plate was obtained: a fast sensor variant detects disturbance through sampling, whereas the slow sensor indicates oxygen limitation during incubation. A combination of the commercially available OxoPlate and the Slow OxoPlate enables operators of screening facilities to validate their cultivation procedures with regard to oxygen availability.
Issue Date: 2015
Date Available: 23-Oct-2017
DDC Class: 620 Ingenieurwissenschaften und zugeordnete Tätigkeiten
Subject(s): oxygen sensor
response time
high throughput
microbial cultures
Sponsor/Funder: BMBF, 02PJ1150, Plattformtechnologien für automatisierte Bioprozessentwicklung (AutoBio)
Journal Title: Journal of laboratory automation
Publisher: Sage Publications
Publisher Place: London [u.a.]
Volume: 20
Issue: 4
Publisher DOI: 10.1177/2211068215573924
Page Start: 438
Page End: 446
EISSN: 2211-0682
ISSN: 1540-2452
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:FG Mess- und Regelungstechnik » Publications
FG Bioverfahrenstechnik » Publications

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