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Main Title: Energetic contribution to both acidity and conformational stability in peptide models
Author(s): Kubyshkin, Vladimir
Durkin, Patrick
Budisa, Nediljko
Type: Article
Language Code: en
Abstract: The acidity of N-acyl amino acids is dependent upon the rotameric state of the amide bond. In this work we systematically investigated the acidity difference of the rotamers (Delta pK(a)) in the frames of various acetylated amino acids. Our results indicated a mutual interaction of two carbonyl groups of an attractive type. We observed conservative Delta pK(a)s for acyclic amino acids (2.2-3.0 kJ mol(-1)), whereas in the case of alicyclic amino acids, the experimental values revealed a strong dependency on the structural context (1.5-4.4 kJ mol(-1)). In homologous amino acids (alpha-, beta-, gamma-, etc.), the strength of the attraction decays in an exponential fashion. Furthermore, the interaction can accumulate through a chain of amide bonds in a cascade fashion, as demonstrated by an Ac-Pro-Pro dipeptide. As a result, we demonstrate that Delta pK(a) is an experimental parameter to estimate increments in the carbonyl-carbonyl alignment, as determined by the amino acid or peptidyl context. This parameter is also important in understanding the roles of amino acids in both protein folding and translation in biological systems as well as their evolutionary appearance in the genetic code.
Issue Date: 2016
Date Available: 24-Oct-2017
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Sponsor/Funder: DFG, EXC 314, Unifying Concepts in Catalysis
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Journal Title: New journal of chemistry
Publisher: Royal Society of Chemistry
Publisher Place: Cambridge
Volume: 40
Issue: 6
Publisher DOI: 10.1039/c5nj03611a
Page Start: 5209
Page End: 5220
EISSN: 1369-9261
ISSN: 1144-0546
Appears in Collections:Fachgebiet Biokatalyse » Publications

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