Please use this identifier to cite or link to this item: http://dx.doi.org/10.14279/depositonce-6478
Main Title: Automated universal chip platform for fluorescence based cellular assays
Author(s): Schmieder, F.
Schmieder, S.
Eger, R.
Friedrich, S.
Werner, A.
Danz, N.
Marx, Uwe
Sonntag, Frank
Type: Article
Language Code: en
Abstract: The advantage of cell based assays used as biosensors is the direct access to hardly obtainable parameters like toxicity, mutagenicity and pharmacological effectiveness. Within the last few years we established a micro fluidic platform including a peristaltic micro pump as well as several valves, manifolds and micro channels [1]. For optical online monitoring the micro fluidic system is bonded to a glass slide. Furthermore the biochip is fixed on an electrically heated support. The pneumatically actuated peristaltic pump as well as the temperature control is performed by a control device. For the fluorescence based online monitoring a robotic guided fluorescence measurement module was developed, which supports the detection of fluorescence in microtiter plates and microfluidic systems. This measurement module allows the fluorescence detection of two different excitation / detection wavelengths (480 / 530 nm and 570 / 620 nm) and was successfully characterised using EGFP and Rhodamine 6G. Additionally three cell based assays with bacterial, yeast and human cells were characterized.
URI: https://depositonce.tu-berlin.de//handle/11303/7203
http://dx.doi.org/10.14279/depositonce-6478
Issue Date: 2012
Date Available: 28-Nov-2017
DDC Class: 610 Medizin, Gesundheit
Usage rights: Terms of German Copyright Law
Journal Title: Biomedical engineering = Biomedizinische Technik
Publisher: De Gruyter
Publisher Place: Berlin [u.a.]
Volume: 57
Issue: Suppl. 1
Publisher DOI: 10.1515/bmt-2012-4319
Page Start: 340
Page End: 343
EISSN: 0013-5585
ISSN: 1862-278X
Notes: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Appears in Collections:Fachgebiet Medizinische Biotechnologie » Publications

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