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Main Title: Micro-electromechanical affinity sensor for the monitoring of glucose in bioprocess media
Author(s): Theuer, Lorenz
Lehmann, Micha
Junne, Stefan
Neubauer, Peter
Birkholz, Mario
Type: Article
Language Code: en
Abstract: An affinity-viscometry-based micro-sensor probe for continuous glucose monitoring was investigated with respect to its suitability for bioprocesses. The sensor operates with glucose and dextran competing as binding partner for concanavalin A, while the viscosity of the assay scales with glucose concentration. Changes in viscosity are determined with a micro-electromechanical system (MEMS) in the measurement cavity of the sensor probe. The study aimed to elucidate the interactions between the assay and a typical phosphate buffered bacterial cultivation medium. It turned out that contact with the medium resulted in a significant long-lasting drift of the assay’s viscosity at zero glucose concentration. Adding glucose to the medium lowers the drift by a factor of eight. The cglc values measured off-line with the glucose sensor for monitoring of a bacterial cultivation were similar to the measurements with an enzymatic assay with a difference of less than ±0.15 g·L−1. We propose that lectin agglomeration, the electro-viscous effect, and constitutional changes of concanavalin A due to exchanges of the incorporated metal ions may account for the observed viscosity increase. The study has demonstrated the potential of the MEMS sensor to determine sensitive viscosity changes within very small sample volumes, which could be of interest for various biotechnological applications.
Issue Date: 2017
Date Available: 17-Jan-2018
DDC Class: 570 Biowissenschaften; Biologie
540 Chemie und zugeordnete Wissenschaften
Subject(s): bioprocess
affinity assay
glucose monitoring
concanavalin A
bacterial culture media
Sponsor/Funder: DFG, TH 662/19-1, Open Access Publizieren 2017 - 2018 / Technische Universität Berlin
Journal Title: International journal of molecular sciences
Publisher: Molecular Diversity Preservation International
Publisher Place: Basel
Volume: 18
Issue: 6
Article Number: 1235
Publisher DOI: 10.3390/ijms18061235
ISSN: 1422-0067
Appears in Collections:FG Bioverfahrenstechnik » Publications

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