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Main Title: Comparative venomics of the Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni from Turkey provides insights into kinship
Author(s): Hempel, Benjamin-Florian
Damm, Maik
Göçmen, Bayram
Karis, Mert
Oguz, Mehmet Anıl
Nalbantsoy, Ayse
Süßmuth, Roderich D.
Type: Article
Language Code: en
Abstract: The Nose-horned Viper (Vipera ammodytes) is one of the most widespread and venomous snakes in Europe, which causes high frequent snakebite accidents. The first comprehensive venom characterization of the regional endemic Transcaucasian Nose-horned Viper (Vipera ammodytes transcaucasiana) and the Transdanubian Sand Viper (Vipera ammodytes montandoni) is reported employing a combination of intact mass profiling and bottom-up proteomics. The bottom-up analysis of both subspecies identified the major snake protein families of viper venoms. Furthermore, intact mass profiling revealed the presence of two tripeptidic metalloprotease inhibitors and their precursors. While previous reports applied multivariate analysis techniques to clarify the taxonomic status of the subspecies, an accurate classification of Vipera ammodytes transcaucasiana is still part of the ongoing research. The comparative analysis of the viper venoms on the proteome level reveals a close relationship between the Vipera ammodytes subspecies, which could be considered to clarify the classification of the Transcaucasian Nose-horned Viper. However, the slightly different ratio of some venom components could be indicating interspecific variations of the two studied subspecies or intraspecies alternations based on small sample size. Additionally, we performed a bioactivity screening with the crude venoms against several human cancerous and non-cancerous cell lines, which showed interesting results against a human breast adenocarcinoma epithelial cell line. Several fractions of Vipera a. transcaucasiana demonstrated a strong cytotoxic effect on triple negative MDA MB 231 breast cancer cells.
Issue Date: 2018
Date Available: 19-Jan-2018
DDC Class: 610 Medizin und Gesundheit
597 Wechselwarme Wirbeltiere; Pisces (Fische)
Subject(s): Viperidae
Vipera ammodytes
Transcaucasian Nose-horned Viper
Transdanubian Sand Viper
snake venomics
intact mass profiling
tripeptide metalloprotease inhibitor
Sponsor/Funder: DFG, TH 662/19-1, Open Access Publizieren 2017 - 2018 / Technische Universität Berlin
Journal Title: Toxins
Publisher: MDPI
Publisher Place: Basel
Volume: 10
Issue: 1
Article Number: 23
Publisher DOI: 10.3390/toxins10010023
ISSN: 2072-6651
Appears in Collections:FG Biologische Chemie » Publications

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