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Main Title: Direct electron irradiation of DNA in a fully aqueous environment. Damage determination in combination with Monte Carlo simulations
Author(s): Hahn, Marc Benjamin
Meyer, Susann
Schröter, Maria-Astrid
Seitz, Harald
Kunte, Hans-Jörg
Solomun, Tihomir
Sturm, Heinz
Type: Article
Language Code: en
Abstract: We report on a study in which plasmid DNA in water was irradiated with 30 keV electrons generated by a scanning electron microscope and passed through a 100 nm thick Si3N4 membrane. The corresponding Monte Carlo simulations suggest that the kinetic energy spectrum of the electrons throughout the water is dominated by low energy electrons (<100 eV). The DNA radiation damage, single-strand breaks (SSBs) and double-strand breaks (DSBs), was determined by gel electrophoresis. The median lethal dose of D1/2 = 1.7 ± 0.3 Gy was found to be much smaller as compared to partially or fully hydrated DNA irradiated under vacuum conditions. The ratio of the DSBs to SSBs was found to be 1 : 12 as compared to 1 : 88 found for hydrated DNA. Our method enables quantitative measurements of radiation damage to biomolecules (DNA, proteins) in solutions under varying conditions (pH, salinity, co-solutes) for an electron energy range which is difficult to probe by standard methods.
Issue Date: 2016
Date Available: 19-Apr-2018
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Journal Title: Physical Chemistry Chemical Physics
Publisher: Royal Society of Chemistry (RSC)
Publisher Place: Cambridge
Volume: 19
Issue: 3
Publisher DOI: 10.1039/c6cp07707b
Page Start: 1798
Page End: 1805
EISSN: 1463-9084
ISSN: 1463-9076
Appears in Collections:FG Tribologie » Publications

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