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Main Title: Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids
Author(s): Ma, Ying
Thota, Bala N. S.
Haag, Rainer
Budisa, Nediljko
Type: Article
Language Code: en
Abstract: Polyglycerol dendrimers as an important class of polymeric materials especially attractive for covalent attachment to therapeutic proteins as a useful alternative to traditional PEGylation procedures. Herein, we combine in vivo noncanonical amino acid (ncAA) incorporation and chemoselective conjugation in vitro to produce novel hybrid protein–dendrimer conjugates with the defined architectures. We incorporated Azidohomoalanine (Aha) as methionine substitute in vivo into various protein scaffolds to allow non-invasive dendrimer conjugations (dendronylation). Our approach makes recombinant proteins accessible for the design of multivalent dendrimer conjugates since it enables the preparation of many sequences with various positions for regioselective dendronylation.
Issue Date: 2015
Date Available: 7-May-2018
DDC Class: 540 Chemie und zugeordnete Wissenschaften
Subject(s): click-chemistry
noncanonical amino acids
oligoglycerol dendrons
protein–dendron conjugate
unnatural protein expression
Journal Title: Bioorganic and Medicinal Chemistry Letters
Publisher: Elsevier
Publisher Place: Amsterdam
Volume: 25
Issue: 22
Publisher DOI: 10.1016/j.bmcl.2015.09.055
Page Start: 5247
Page End: 5249
ISSN: 0960-894X
Appears in Collections:FG Biokatalyse » Publications

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