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Main Title: Conditional expression of the small GTPase ArfA impacts secretion, morphology, growth, and actin ring position in Aspergillus niger
Author(s): Fiedler, Markus R. M.
Cairns, Timothy C.
Koch, Oliver
Kubisch, Christin
Meyer, Vera
Type: Article
Language Code: en
Abstract: In filamentous fungi, growth and protein secretion occurs predominantly at the tip of long, thread like cells termed hyphae. This requires coordinated regulation of multiple processes, including vesicle trafficking, exocytosis, and endocytosis, which are facilitated by a complex cytoskeletal apparatus. In this study, functional analyses of the small GTPase ArfA from Aspergillus niger demonstrate that this protein functionally complements the Saccharomyces cerevisiae ARF1/2, and that this protein is essential for A. niger. Loss-of-function and gain-of-function analyses demonstrate that titration of arfA expression impacts hyphal growth rate, hyphal tip morphology, and protein secretion. Moreover, localization of the endocytic machinery, visualized via fluorescent tagging of the actin ring, was found to be abnormal in ArfA under- and overexpressed conditions. Finally, we provide evidence that the major secreted protein GlaA localizes at septal junctions, indicating that secretion in A. niger may occur at these loci, and that this process is likely impacted by arfA expression levels. Taken together, our results demonstrate that ArfA fulfills multiple functions in the secretory pathway of A. niger.
Issue Date: 8-May-2018
Date Available: 17-May-2018
DDC Class: 570 Biowissenschaften; Biologie
Subject(s): Aspergillus niger
protein secretion
conditional gene expression
Sponsor/Funder: DFG, TH 662/19-1, Open Access Publizieren 2017 - 2018 / Technische Universität Berlin
Journal Title: Frontiers in Microbiology
Publisher: Frontiers Media
Publisher Place: Lausanne
Volume: 9
Article Number: 878
Publisher DOI: 10.3389/fmicb.2018.00878
Page Start: 1
Page End: 17
ISSN: 1664-302X
Appears in Collections:FG Angewandte und Molekulare Mikrobiologie » Publications

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